Reductionism VS Holism The study of biology through systematic perturbation, global read-out of the multifaceted and integration of these data to formulate predictive models Ideker et al., Annu. Rev. Genomics Hum. Genet. 2:343-72, 2001.
Integrating “omic” approaches Ge H et al., 2003.
Take home message 1.System biology is a post-genomic field that focus in global changes within a system. 2.Depends heavily on computational approaches (measurement, statistical validation, integration of thousands of components) 3.Proteomics is still “under development” compared to Genomics/transcriptomics, but has almost comparable throughput in recent years
Advanced Proteomics October 2013 Gustavo de Souza IMM, OUS
Are mRNA levels comparable to their protein levels?
Relevance of the Proteome If a gene is transcripted and its mRNA levels goes up, does that necessarily means the protein level that the mRNA translates also goes up?
Take home message - As a descriptive data, proteomics is almost as throughput as transcriptomics (the catch 22 is: it does require very high level of expertise to perform proteomics at higher standards). - Proteomics is arguably more appropriate than transcriptomics to elucidate functional studies.