What’s the difference between DNA and RNA? 1.RNA is single stranded. 2.RNA can create secondary structures other than a double helix. 3.RNA = Uracil. DNA = thymine. 4.RNA = ribonucleic acid. DNA = deoxyribonucleic acid. 5.RNA is temporary.
Division Limitation: Telomeres 1. why is this good for cells in the body? 2. what challenges does this make for tissue engineering? 21
How do cells come together to create functional organisms? Cell: the basic unit of all living things. Tissue: group of similar cells and the materials surrounding them. Organ: composed of two or more tissue types that perform one or more common functions. – E.g. bladder, heart, skin Organ system: group of organs that have a common function or set of functions and are therefore viewed as a unit. Humans have 11 major organ systems Organism: any living thing considered as a whole.
Tissue Engineering Challenge: Very difficult to get a drug through the skin! Integumentary System skin, hair, nails, and sweat glands Chemical Engineering Analog: Heat Exchanger Humidifier Solar Panels (Vit D)
Skeletal System first to be “engineered” Traditional route for skeletal system engineering: Bio-inert materials Purposes: 1.Replace tissue structure and function, not bio-activity 2.do not entice an immune response once implanted into the body. 3.Have incredible mechanical toughness withstand physiological loading 4.Long lasting in the body (won’t degrade over time) Applications: Skeletal tissue prosthesis (hip, knee replacement) 24 What types of materials would be suitable? Non-corroding metals (titanium, stainless steel) Certain Ceramics Polymer Composites
Muscular System Produces body movements, maintains posture, and produces body heat. Consists of muscles attached to the skeleton by tendons Not truly engineered yet. Many working on stem cell-to-muscle cell differentiation techniques.
Nervous System A major regulatory system that detects sensations and controls movements, physiologic processes, and intellectual functions. Consists of the brain, spinal cord, nerves, and sensory receptors. BrainGate Neural Interface
Endocrine System A major regulatory system that influences metabolism, growth, reproduction, and many other functions. Consists of glands, such as the pituitary, that secrete hormones Not truly engineered yet. Typically treated with repeated injections of hormones or daily pills. Can be adapted to a patch or implanted, permanent device? Stem cell differentiation to islet cells Chemical Engineering Analog (Pancreas): Control System (Positive Feedback Loop)
Heart Valves, from cadavers or animals Rat heart lungs Cardiovascular and Respiratory Systems
Lymphatic System Removes foreign substances from the blood and lymph, combats disease, maintains tissue fluid balance, and absorbs fats from the digestive tract. Consists of the lymphatic vessels, lymph nodes, and other lymphatic organs. Only bioengineer (Chemical Engineer) working on this is Melody Swartz, EPFL. She studies flow patterns to the lymph using principles of fluid dynamics. Chemical Engineering Analog: Filtration Unit (ion exchanger/separator) Tank (collects fluid) Control unit: Positive feedback loop (recruitment of immune cells)
Digestive System mouth, esophagus, stomach, intestines, and accessory organs (2) Filtrate (3) Clean blood out (1) Toxin + blood in Synthetic Filter: retains very large compounds (>100,000 g/mol) + Hepatocytes: process bilirubin (reaction) Filter + hepatocytes (4) Back to patient Chemical Engineering Analog: Filtration Unit (liquid phase extraction)
Urinary System kidneys, urinary bladder, and ducts that carry urine. Chemical Engineering Analog: Filtration Unit (ion exchanger/separator) Continuous flow (or semi-batch) reactor
Cell Types – Quick Breakdown
Muscle Cells: 3 Types Contraction, Movement
Nerve Cells: Information Relay
____blasts and ____cytes and ____clasts: Sense, Turnover, replace tissue 35 Skin = FIBROblast Cartilage = CHONDROcyte Bone = OSTEOcyte, OSTEOblast, OSTEOclast
Epithelial Cell: Barriers, separation
Now you’re ready! Find a tissue in need Establish design criteria – what does it do and what are the key functions? Cells (?) + Material
Wiki SignUp from Tuesday 1.Get account! 2.First one Thursday! 3.Page due same day, 4p 4.Open slots: extra credit 1/3 letter grade
Paper SignUp from Tuesday Sign up sheet is online, too big to show here First one Tuesday! Everyone read the paper! Open slots: extra credit – 1/3 letter grade