Presentation on theme: "Enterobacteriaceae Slackers Facts by Mike Ori. Disclaimer The information represents my understanding only so errors and omissions are probably rampant."— Presentation transcript:
Disclaimer The information represents my understanding only so errors and omissions are probably rampant. It has not been vetted or reviewed by faculty. The source is our class notes. The document can mostly be used forward and backward. I tried to mark questionable stuff with (?). If you want it to look pretty, steal some crayons and go to town. Finally… If you’re a gunner, buck up and do your own work.
Interferes with protein synthesis: An A-B toxin secreted by E. coli whose active unit interferes with protein synthesis by blocking the ribosomal amino acyl tRNA binding site. Causes hemolysis by unknown mechanisms. Interferes with kidney function: Circulating toxin binds renal tissues causing glomerular swelling and the deposition of fibrin and platelets into the microvasculature.
Significant cause of mortality in children 2 and younger in less developed countries. Common cause of traveler’s diarrhea. Human reservoir. Point to ponder: What kind of diarrhea would the time traveler’s wife’s husband have?
Bundle forming pili (BFP) bearing intimin protein mediate attachment of the cells to host enterocytes. Subsequent injection of E. coli secretion proteins (Esp) causes the formation of a pedestal by modifying the cytoskeleton.
Lesion caused by EPEC and EHEC E. coli strains by injection of E. coli secretory proteins (Esp) that results in the effacement of enterocyte microvilli and the attachment of E. coli at the top of pedestals formed from commandeered cytoskeletal components.
Supportive care is mainstay Replace fluids with oral rehydration therapy or IV if necessary. Abx for ETEC, EIEC, EPEC but NOT for EHEC Antimotility agents if you have to for EPEC and ETEC but NOT for EIEC of EHEC
Acid resistant (not acid fast) gram negative rod transmitted by person to person contact under conditions of poor sanitation which causes disease that begins with watery diarrhea and evolves into dysentery type diarrhea.
Spread by direct person to person contact under conditions of poor personal or community sanitation. Small infecting dose of less than 200 organisms insures efficient transmission. The reservoir is human
Proteins injected into host cells by shigella using a contact initiated injection system. These proteins have a wide range of function including cytoskeletal rearrangement, actin polymerization, and apoptosis
Picnics, warm summer days, drunken co- workers, contaminated food, logarithmic growth, You didn’t eat the potato salad did you? You did? Damn! 2 day incubation, 3-4 days of suffering. See you next year.
Invasion of mucosa Ingestion by macrophages but escape of killing by interfering with oxidative burst/lysosome fusion (??) Travel to secondary lymphoid tissues Rupture of macrophages Dissemination of bacteria Dissemination of LPS endotoxin resulting in fever