Presentation on theme: "VISIT IN TUBINGEN. The retinal preparation process 1. Removing the eye cup of rat 2. Cut the eye cap along the ora serrata 3. Dissect the retina for three."— Presentation transcript:
The retinal preparation process 1. Removing the eye cup of rat 2. Cut the eye cap along the ora serrata 3. Dissect the retina for three segments that will be recorded 4. Loosen the neural retina from the sclera and transfer it to the recording field of the MEA
The perforated MEA Improve retina - electrodes contact. Measure the ganglion cell activity.
The perforated MEA setup The improved contact achieved by applying a negative pressure fro underneath the MEA
MCS system Amplifier and data acquisition. MEAs. Light sources to stimulate the retina. Perfusion setup.
STIMULUS OF UNIFORM LIGHT Light intensity of 3.6klux Cycle time Total time of 30 sec TP=time of pulse f= frequency.
The f and TP values 5ms50ms100ms500ms 0.25Hz 0.5Hz 1Hz 5Hz 10Hz 20Hz
The signal processing block diagram FFT TRANSFORMATION ROW ELECTRODE FILTER 200Hz-3kHz reconstruction Frequency space Frequency space (peak detector) Level filter 50mv Time space Process the date (frequency, number of AP, width of the respond …)
Compare between 50,100,500 ms pulse at 0.5Hz from one electrode number 47