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Light Pollution Project THIN LAYER CHROMATOGRAPHY S 3 2002, Visnjan.

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Presentation on theme: "Light Pollution Project THIN LAYER CHROMATOGRAPHY S 3 2002, Visnjan."— Presentation transcript:

1 Light Pollution Project THIN LAYER CHROMATOGRAPHY S , Visnjan

2 Light Pollution Project - TLC T HEORY Photosynthesis - process which plants use to produce carbohydrates 6H 2 O + 6CO > C 6 H 12 O 6 + 6O 2 - light is necessary for the process - light is absorbed by plant pigments

3 Light Pollution Project - TLC T HEORY Chlorophyll - the major plant pigment Accessory pigments- e.g. carotenoids

4 Light Pollution Project - TLC E XPERIMENTS - we analyzed the influence of light on concentration of the chlorophyll 1. LABORATORY CONDITIONS - cucumber - tungsten and Na lamps - 24 h under the lamps 16 h outside and 8 h under the lamps regular daily cycle

5 Light Pollution Project - TLC E XPERIMENTS NATURAL CONDITIONS - compare the concentration of chlorophyll between plants of the same species on “Istarski Y” and referential field - these two locations are similar because of the artificial lake for water treatment - different geological and hydrological elements

6 Light Pollution Project - TLC E XPERIMENTS TLC (thin layer chromatography) - after extraction of the chlorophyll from plants, the samples were put on TLC plates - development of plates - calculation of areas of the spots - the area of the spot is proportional to the concentration of the chlorophyll in the sample

7 Light Pollution Project - TLC R ESULTS - plants under both types of plants have lower concentration of chlorophyll then the control ones LABORATORY CONDITIONS

8 Light Pollution Project - TLC R ESULTS NATURAL CONDITIONS - for different species, light has different effect on the concentration of chlorophyll

9 Light Pollution Project - TLC R ESULTS

10 Light Pollution Project - TLC C ONCLUSION - when plants are illuminated during the night the concentration of chlorophyll reduces - there is only one measurement for each type of plant and there is a big chance that mistakes occur - sodium lamps increase the amount of chlorophyll of some plants (e.g. cichorium and daucus), to some don’t have any influence (e.g. plantago) and to some decrease the amount of chlorophyll(e.g. lotus and asteraceae) - the important thing is that software for calculating areas very precisely is developed


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