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3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,

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Presentation on theme: "3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,"— Presentation transcript:

1 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203, FALL 2014

2 Exp Isolation of Chlorophyll and Carotenoids from Spinach

3 Chloroplasts contain colored compounds (pigments) which fall into two categories chlorophylls and carotenoids

4 Exp Isolation of Chlorophyll and Carotenoids from Spinach Chlorophyll b: -CHO group instead of shaded methyl group Pheophytin a: identical to chlorophyll a except that Mg 2+ is replaced by two H + Pheophytin b: identical to chlorophyll b except that Mg 2+ is replaced by two H +

5 Exp Isolation of Chlorophyll and Carotenoids from Spinach

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7  -Carotene  -Carotene  -Carotene Different position of the double bond Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.

8 Exp Isolation of Chlorophyll and Carotenoids from Spinach Carotene is responsible for the orange colour of the carrots and many other fruits and vegetables. Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.

9 Exp Isolation of Chlorophyll and Carotenoids from Spinach

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11 Exp Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

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24 Exp Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography

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