Presentation on theme: "The Effect of Beta Carotene on Plants Infected with Agrobacterium Tumefaciens Christina Adams Grade 9."— Presentation transcript:
1The Effect of Beta Carotene on Plants Infected with Agrobacterium Tumefaciens Christina AdamsGrade 9
2ProblemWill Beta Carotene have any role in preventing the effect of Agrobacterium tumefaciens on violets?I picked this experiment because of my interest in Botany and how different bacteria and solutions can affect the plant’s growth.
3ResearchBeta Carotene is a red-orange pigment found in various types of plants. Beta Carotene is converted in the body to Vitamin A.Agrobacterium tumefaciens is the bacteria that causes tumor formation in over 140 different dicot plants (plants with 2 or more embyrotic leaves.)A. tumefaciens can live freely in soil or inside plants as a parasite; Causes disease by transferring its own DNA into plants cells.Most A. tumefaciens infections caused by wounds in the plants (result from grafting together different plant stocks.)
4HypothesisIf Beta Carotene is used, then it should have no effect on preventing damage from the Agrobacterium tumefaciens. Beta Carotene
6Procedure 8 plants were placed in each pot Pot A is inoculated with A. tumefaciens and watered with 100ml of beta carotene twice weekly.Pot B is inoculated with A. tumefaciens and watered with 100ml of distilled water twice weekly.Pot C is not inoculated, but watered with 100ml of distilled water twice weekly.The height of the plants will be documented over a 30-day period.
7Independent and Dependent Variables Independent variable: what the seeds are germinated inDependent variable: the height and growth rate of the plantsControl: Group C (water control group)
11ConclusionIf Beta Carotene is used then it will have no effect on A. tumefaciens infections.This was not supported.The control group had the highest growth rate followed by the beta carotene group.The bacterial group had the lowest growth rate-Growth stopped after two weeks and plants began to brown
12Conclusion (cont) How to improve experiment: - use different types of plants, such as monocot plants, and different types of solution to test the effectiveness of prevention.- Test the effectiveness of the Beta Carotene on infected plants in higher doses.- Test Beta Carotene on humans with cancer.
13Works CitedBochinski, Julianne Blair. The Complete Handbook of Science Fair Projects. N.p.: n.p., n.d. Print.Helling, Christopher H. “How Effective is Beta Carotene in Fighting Cancer in Plants?” California State Science Fair 2008 Project Summary. N.p., n.d. Web. 5 OctTzfira, Tzvi, and Vitaly Citovsky. “Taking Biology Lessons from a Bug.” The Agrobacterium- Plant Cell Interaction. N.p., n.d. Web. 6 OctUniversity of Minnesota- Department of Pediatrics. “Agrobacterium Infections in Humans.” University of Minnesota. N.p., n.d. Web. 6 OctVarious school students. “Do Plants Get Cancer?” Student Sheet. N.p., n.d. Web. 6 OctThank you for listening!