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The LD50 of Common Household Toxins on Brine Shrimp Kailin Edgar May 17th, 2012.

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Presentation on theme: "The LD50 of Common Household Toxins on Brine Shrimp Kailin Edgar May 17th, 2012."— Presentation transcript:

1 The LD50 of Common Household Toxins on Brine Shrimp Kailin Edgar May 17th, 2012

2 Brine Shrimp Tolerate a salt content of between 25 and 35% salt Tolerate a salt content of between 25 and 35% salt Can be found in salt flats Can be found in salt flats Important food source to many wildlife species Important food source to many wildlife species Indicators of pollution within water bodies Indicators of pollution within water bodies

3 LD50 50% of an organism is killed by a specific concentration of a toxin 50% of an organism is killed by a specific concentration of a toxin Higher threshold=withstand higher concentrations Higher threshold=withstand higher concentrations

4 Problem What is the LD50 of common household toxins on brine shrimp? What is the LD50 of common household toxins on brine shrimp?

5 Hypothesis If Brine Shrimp are placed in various concentrations of common household toxins such as Lysol, bleach, and laundry detergent then the bleach will be the most harmful product and the laundry detergent will be less harmful (shrimp will have the highest tolerance for the laundry detergent). If Brine Shrimp are placed in various concentrations of common household toxins such as Lysol, bleach, and laundry detergent then the bleach will be the most harmful product and the laundry detergent will be less harmful (shrimp will have the highest tolerance for the laundry detergent).

6 Materials 1000 ml beaker 1000 ml beaker Eye droppers Eye droppers 10 ml pipettes 10 ml pipettes Graduated cylinders Graduated cylinders Small petri dishes Small petri dishes Beakers Beakers Stop watch Stop watch Live brine shrimp Live brine shrimp Pipump Pipump Lysol Lysol Bleach Bleach Washing Detergent Washing Detergent

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8 Procedure 1. Obtain brine shrimp 2. Label petri dishes 0%, 10%, 20%, 30%, 40%, and 50% 3. Place 10mL of water in the 0% petri dish 4. Place 9mL of water in the petri dish labeled 10% and then add 1mL of bleach 5. Place 8mL of water in the petri dish labeled 20% and then add 2mL of bleach 6. Place 7mL of water in the petri dish labeled 30% and then add 3mL of bleach 7. Place 6mL of water in the petri dish labeled 40% and then add 4mL of bleach 8. Place 5mL of water in the petri dish labeled 50% and then add 5mL of bleach

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10 Procedure 9. Add 30 brine shrimp to each petri dish 10. Let shrimp sit in water for 5 minutes 11. Count how many shrimp are still alive in each concentration of the toxin 12. Record data and behavior 13. Compare results 14. Repeat steps 2-14 for each toxin

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12 Data

13 Data

14 Data Concentration of Toxin (%) Number of Shrimp Alive after 5 minutes

15 Discussion Hypothesis was supported Hypothesis was supported Bleach was the most harmful toxin  killed more than half of the shrimp population in a 10% concentration Bleach was the most harmful toxin  killed more than half of the shrimp population in a 10% concentration Lysol was very toxic but threshold was higher  more than 50% were killed in a 20% concentration Lysol was very toxic but threshold was higher  more than 50% were killed in a 20% concentration Washing detergent was least harmful  LD50 was between 40-50% Washing detergent was least harmful  LD50 was between 40-50%

16 Sources of error LD50’s are not exact because concentrations were spread apart LD50’s are not exact because concentrations were spread apart Determining whether the shrimp were still alive was difficult and counting the same shrimp twice was very possible Determining whether the shrimp were still alive was difficult and counting the same shrimp twice was very possible

17 Conclusion Household products can be very toxic Household products can be very toxic Small marine animals exposed to small concentrations of a product may be killed or their swimming severly affected Small marine animals exposed to small concentrations of a product may be killed or their swimming severly affected

18 Future Implications The effect of household toxins on larger marine animals The effect of household toxins on larger marine animals New, environmentally safe products can be invented to take the place of these toxins without causing the same effects New, environmentally safe products can be invented to take the place of these toxins without causing the same effects Ways to expose of these toxins  keep them out of the water Ways to expose of these toxins  keep them out of the water

19 Questions?


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