Presentation on theme: "The LD50 of Common Household Toxins on Brine Shrimp"— Presentation transcript:
1The LD50 of Common Household Toxins on Brine Shrimp Kailin EdgarMay 17th, 2012
2Brine Shrimp Tolerate a salt content of between 25 and 35% salt Can be found in salt flatsImportant food source to many wildlife speciesIndicators of pollution within water bodies
3LD5050% of an organism is killed by a specific concentration of a toxinHigher threshold=withstand higher concentrations
4ProblemWhat is the LD50 of common household toxins on brine shrimp?
5HypothesisIf Brine Shrimp are placed in various concentrations of common household toxins such as Lysol, bleach, and laundry detergent then the bleach will be the most harmful product and the laundry detergent will be less harmful (shrimp will have the highest tolerance for the laundry detergent).
6Materials 1000 ml beaker Stop watch Eye droppers Live brine shrimp 10 ml pipettesPipumpGraduated cylindersLysolSmall petri dishesBleachBeakersWashing Detergent
8Procedure Obtain brine shrimp Label petri dishes 0%, 10%, 20%, 30%, 40%, and 50%Place 10mL of water in the 0% petri dishPlace 9mL of water in the petri dish labeled 10% and then add 1mL of bleachPlace 8mL of water in the petri dish labeled 20% and then add 2mL of bleachPlace 7mL of water in the petri dish labeled 30% and then add 3mL of bleachPlace 6mL of water in the petri dish labeled 40% and then add 4mL of bleachPlace 5mL of water in the petri dish labeled 50% and then add 5mL of bleach
10Procedure Add 30 brine shrimp to each petri dish Let shrimp sit in water for 5 minutesCount how many shrimp are still alive in each concentration of the toxinRecord data and behaviorCompare resultsRepeat steps 2-14 for each toxin
14DataNumber of Shrimp Alive after 5 minutesConcentration of Toxin (%)
15Discussion Hypothesis was supported Bleach was the most harmful toxin killed more than half of the shrimp population in a 10% concentrationLysol was very toxic but threshold was higher more than 50% were killed in a 20% concentrationWashing detergent was least harmful LD50 was between 40-50%
16Sources of errorLD50’s are not exact because concentrations were spread apartDetermining whether the shrimp were still alive was difficult and counting the same shrimp twice was very possible
17Conclusion Household products can be very toxic Small marine animals exposed to small concentrations of a product may be killed or their swimming severly affected
18Future ImplicationsThe effect of household toxins on larger marine animalsNew, environmentally safe products can be invented to take the place of these toxins without causing the same effectsWays to expose of these toxins keep them out of the water