Presentation on theme: "Adding Value to Molasses Donal Day and C. Chung Audubon Sugar Institute Audubon Sugar Institute Factory Operations Seminar 2007."— Presentation transcript:
Adding Value to Molasses Donal Day and C. Chung Audubon Sugar Institute Audubon Sugar Institute Factory Operations Seminar 2007
Goal Upgrade the value of molasses by converting the sugar to a higher value product Target Market-Quality Liquid Feeds Others- Human and poultry feeds
Background We have patented a process that produces an oligosaccharide mixture from sugar. This mixture has shown beneficial effects in animal (poultry) nutrition
L. mesenteroides B-742 ( ATCC 13146) Synthesizes a highly branched dextran from sucrose High yields of oligosaccharide from sucrose in presence of maltose by acceptor reaction Fructose conversion to mannitol
Oligosaccharide Production-Process Flow Fermentation with L. mesenteroides ATCC 13146 Cell separation Product polishing by chromatography Oligosaccharide product Deionization and Decolorization Mannitol crystallization Input : maltose 50 g/L sucrose 100 g/L, salts Output : oligosaccharides 80.1 g/L (82.3 % theoretical yield) mannitol 37.6 g/L (70.1 % conversion ) 86.4 % mannitol recovered Ca 2+ ion exchange 98.8 % purity
Oligosaccharide Distribution by DP % Degree of Polymerization
Postulated structures of isomaltooligosaccharides DP, Degree of Polymerization DP3 (panose). -1,6 o o o o o oo o o o oo o o -1,4 -1,3 (?) o (glucose).
Isomalto(gluco)oligosac charide Process Rapid, simple and economical production method Readily Scalable High production of glucooligosaccharides and mannitol
Proposed Mechanism of Action IMO slows starch utilization (hence growth of undesirable microflora) Growth of desirable microflora favored Intestinal pH drop produces “wash-out” of non-favored species Acts as a -glucosidase inhibitor, blocking maltose translocation
In Vivo Experimentation Toxicity and weight gain in rats LSU Weight gain, Salmonella loading in poultry (broilers) - USDA, Athens, Ga.
Rat Toxicity Trials No toxicity seen No abnormal physiology on sacrifice Animals liked the material
What’s Next? Contacting QLF to set up trials of material in their process. Investigation of “health benefits” of new material in animal market Pure Material- pursuing possibility of GRAS status with FDA Research into human food applications
Acknowledgement This work was supported in part by the American Sugar Cane League Dr. Roy Martin and Kathleen McCutcheon for their help with the rat studies Dr. Greg Siragusa, USDA, Athens, Ga for his help with the poultry trials