Presentation on theme: "THCA in Head Hair: A Comparison with Fingernail. Conflict of Interest Employees of USDTL Privately held company Commercial laboratory Sells hair testing."— Presentation transcript:
THCA in Head Hair: A Comparison with Fingernail
Conflict of Interest Employees of USDTL Privately held company Commercial laboratory Sells hair testing services Internally funded No role in: Study design Data collection Analysis publication
Marijuana usage trends THCA in hair and nail Validation w 10 fg/mg LOD Comparison hair and nail Conclusions OBJECTIVES
Most common illicit drug M past month users 2009 factor 376k ED visits Trends of Marijuana Use
Trends of Marijuana Use 23 States and DC Legalized Medical use Decriminalized Both
Trends of Marijuana Use Daily use 12 th graders 6.5% from 5.1% in th graders negative perception of regular use 44.1%, lowest since 1979
What Do We Look For? THCTHCA
#1 ENVIRONMENTAL EXPOSURE SMOKE TRANSFERANCE CONTACT FUMES HOW DO DRUGS GET INTO THE HAIR?
#2 SWEAT AND OIL THESE DRUG AND METABOLITE CONTAINING FLUIDS BATHE THE HAIR SHAFT
HOW DO DRUGS GET INTO THE HAIR? #3 THE BLOOD
Specimen Type: Why Fingernail?
Anatomy of Fingernail NAIL MATRIX NAIL PLATE NAIL BED
Fingernails Are Made of Keratin o Keratin: fibrous structural protein of hair, nails, horn, hoofs, wool, feathers, and of the epithelial cells in the outermost layers of the skin. The polypeptide chains of keratin are arranged in parallel sheets held together by hydrogen bonding. ~ Encyclopedia Britannica
Fingernails Are Made of Keratin o These keratin sheets stack on top of each other, giving the nail thickness and length, making adulteration of the specimen difficult.
Incorporation of Drug Biomarker: a measurable substance whose presence is indicative of ingestion or exposure to substances of abuse (SOA).
NAIL MATRIX NAIL BED o Biomarkers can be found in nails as early as 1-2 weeks after substances of abuse are ingested. o The time period during which drug ingestion can be detected depends on the substance used, the amount used, and personal metabolism. Biomarker Absorption
Single point Calibrator – 50 fg/mg LOQ – 20 fg/mg LOD – 10 fg/mg ULOL – 1000 fg/mg Controls 20, 62.5, and 400 fg/mg Robust – minimum 22 consecutive days THCA Validation Specs