Presentation on theme: "THCA in Head Hair: A Comparison with Fingernail. Conflict of Interest Employees of USDTL Privately held company Commercial laboratory Sells hair testing."— Presentation transcript:
Anatomy of Fingernail NAIL MATRIX NAIL PLATE NAIL BED
Fingernails Are Made of Keratin o Keratin: fibrous structural protein of hair, nails, horn, hoofs, wool, feathers, and of the epithelial cells in the outermost layers of the skin. The polypeptide chains of keratin are arranged in parallel sheets held together by hydrogen bonding. ~ Encyclopedia Britannica
Fingernails Are Made of Keratin o These keratin sheets stack on top of each other, giving the nail thickness and length, making adulteration of the specimen difficult.
Incorporation of Drug Biomarker: a measurable substance whose presence is indicative of ingestion or exposure to substances of abuse (SOA).
NAIL MATRIX NAIL BED o Biomarkers can be found in nails as early as 1-2 weeks after substances of abuse are ingested. o The time period during which drug ingestion can be detected depends on the substance used, the amount used, and personal metabolism. Biomarker Absorption
Single point Calibrator – 50 fg/mg LOQ – 20 fg/mg LOD – 10 fg/mg ULOL – 1000 fg/mg Controls 20, 62.5, and 400 fg/mg Robust – minimum 22 consecutive days THCA Validation Specs