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NATURAL FIBROUS MATERIALS AS A SOURCE OF INSPIRATION FOR THE DESIGN OF NOVEL NANOBIOMATERIALS Anna Mitraki Laboratory of Natural Biomaterials Department.

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Presentation on theme: "NATURAL FIBROUS MATERIALS AS A SOURCE OF INSPIRATION FOR THE DESIGN OF NOVEL NANOBIOMATERIALS Anna Mitraki Laboratory of Natural Biomaterials Department."— Presentation transcript:

1 NATURAL FIBROUS MATERIALS AS A SOURCE OF INSPIRATION FOR THE DESIGN OF NOVEL NANOBIOMATERIALS Anna Mitraki Laboratory of Natural Biomaterials Department of Materials Science and Technology, University of Crete, and Institute for Electronic Structure and Lasers, FORTH

2 Our research focuses on: Rational design of protein fibrous materials from the nano- to the macroscopic scale from the nano- to the macroscopic scale using building blocks inspired from using building blocks inspired from Natural fibrous folds and hard-won biochemical knowledge Natural fibrous folds and hard-won biochemical knowledge 50 nm Crystal structure of a natural fibrous protein made from sequence repeats (blue squares) [van Raaij et al. Nature 1999, 401:935]

3 B 100 nm []n]n 50 nm Peptide sequences as a self-assembly system Identification of minimal peptides corresponding to natural building blocks (blue squares) that can self-assemble into fibrous objects Papanikolopoulou et al., J. Biol. Chem. 2005, 280 :

4 Modification of fibrous building blocks towards specific functions: -targeting sequences for metallization, cell attachment etc, or -targeting sequences for metallization, cell attachment etc, or -Conjugation to other organic moieties to form hybrid materials

5 Metal nanowires fabricated on an octapeptide fibrillar scaffold displaying cysteines Kasotakis et al., 2009, Biopolymers-Peptide Science, 92: Example:

6 ….and Development of inter-disciplinary approaches towards controlled positioning on surfaces and integration in devices V. Dinca et al., Nanoletters, (2008) 8 (2): A.Mitraki and M. Farsari, in : "Nanomaterials for Life Sciences" series, vol. 15, pp , Wiley- VCH Example: peptide fibrils positioned on a hybrid organic- inorganic material using femtosecond Laser Technologies

7 Relevant recent publications: 1) Dinca V., et al., (2008) Directed three-dimensional patterning of self-assembled peptide fibrils. Nanoletters, 8: )Dinca V., et al., (2008) Fabrication of amyloid peptide micro-arrays using Laser-induced forward transfer and avidin-biotin mediated assembly Physica Status Solidi 5: )Kasotakis, E., et al., (2009) Design of metal-binding sites onto self-assembled peptide fibrils Biopolymers –Peptide Science 92: )Tamamis, P., et al., (2009) Amyloid-like self-assembly of peptide sequences from the adenovirus fiber shaft: insights from molecular dynamics simulations. J. Phys. Chem. B. 113: ) Mitraki, A. (2010) Protein aggregation: from inclusion bodies to amyloid and biomaterials. Advances in Protein Chemistry and Structural Biology, 79: )Sedman VL., et al., (2011) Surface-Templated Fibril Growth of Peptide Fragments from the Shaft Domain of the Adenovirus Fibre Protein. Protein Pept Lett., 18: ) Tiggelaar, SM., et al., (2011). Neutron fibre diffraction studies of amyloid using H(2)O/D(2)O isotopic replacement. Acta Crystallogr Sect F Struct Biol Cryst Commun. 67: )Hyttel-Clausen C., et al., (2011) Electrostatic force microscopy of self-assembled peptide structures. Scanning, in press 9)Viguier, B., et al., (2011) Development of an electrochemical metal-ion biosensor using self-assembled peptide nanofibrils. ACS Appl. Mater. Interfaces, in press. 10) Charalambidis, G., et al., 2011) Self-assembly into spheres of a hybrid diphenylalanine-porphyrin: increased fluorescence lifetime, conserved electronic properties. Chemistry Eur. J., in press

8 Acknowledgements UoC: Emmanouil Kasotakis Erifyli Kaloudi Dina Terzaki Alexandra Siakouli (EM Facility) Eva Papadogiorgaki (EM Facility) Aleka Manousaki (EM Facility) Collaborations - IESL, FORTH CRETE Dr. Maria Farsari, Prof. Costas Fotakis, - Technical University of Denmark (DTU) Prof. Winnie Svendsen, Dr. Jaime Castillo - Institut Laue Langevin in Grenoble, France Prof. Trevor Forsyth, Estelle Mossou - University of Nottingham, England Prof. Saul Tendler, Prof. Clive Roberts, Dr. Stephanie Allen, Dr. Sedman Victoria - University of Santiago de Compostela,Spain Prof. Mark J. van Raaij - University of Tel-Aviv, Israel Prof. Ehud Gazit, Lihi Adler-Abramovich - University of Cyprus Prof. G. Archontis, Dr. Ph. Tamamis - CEA Saclay, France Dr. P. Guenoun, Cr. C. Chevallard Financial support: EU “BeNatural” project “Bioengineered Nanomaterials For Research and Applications” NMP4-CT Coordinated by UoC


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