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Sustainable Pathways for Algal Bioenergy. Does my algae freeze? Michele Stanley and John Day Scottish Association for Marine Science.

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Presentation on theme: "Sustainable Pathways for Algal Bioenergy. Does my algae freeze? Michele Stanley and John Day Scottish Association for Marine Science."— Presentation transcript:

1 Sustainable Pathways for Algal Bioenergy

2 Does my algae freeze? Michele Stanley and John Day Scottish Association for Marine Science

3 Sustainable Pathways for Algal Bioenergy

4 Biodiversity Biological Resources

5 Sustainable Pathways for Algal Bioenergy (modified from Subitec Value Pyramid for Algae Product Markets in Bruton et al., 2009) Value Pyramid for Algal Derived Products

6 Sustainable Pathways for Algal Bioenergy Case Study Microalgae- derived nutraceuticals Schlarb-Ridley & Parker (2013). A UK Roadmap for Algal Technologies for NERC/TSB Algal Bioenergy Special Interest Group.

7 Sustainable Pathways for Algal Bioenergy Traditional maintenance of algal cultures

8 Sustainable Pathways for Algal Bioenergy

9 Genotypic stability of algae Options available to assess genotypic stability Phenotypic Whole genome Targeted gene Culture stability Morphological changes Changes in productivity Options available to conserve stability Cryopreservation Serial transfer 5 µm

10 Sustainable Pathways for Algal Bioenergy Observed phenotypic changes Loss of spine production Apparently irreversible cell shrinkage in diatoms Loss of ketocarotenoid production Changes in apical cell structure in filamentous cyanobacteria Loss of gas vacuole production Change in phyco-biliprotein composition Loss of alkaloid neurotoxin production

11 Sustainable Pathways for Algal Bioenergy Strain stability Phenotype Phaeocystis antarctica Gäbler-Schwarz et al. Cryoletters (in press)

12 Sustainable Pathways for Algal Bioenergy Why Long-term Preservation Prevent phenotypic change/ loss of important attributes Prevent genotypic change in conserved material Prevent loss of strain Reduce maintenance costs (staff & consumables

13 Sustainable Pathways for Algal Bioenergy Cryopreservation Protocol / methodological development Protocol validation Cryoinjury studies

14 Sustainable Pathways for Algal Bioenergy Evidence of genotypic & functional stability of a transgenic diatom Hipkin et al. (in press) J appl Phycol

15 Sustainable Pathways for Algal Bioenergy AFLP analysis of reference strain and cryopreserved E. gracilis - encapsulation, osmotic dehydration, 4 h desiccation, methanol treatment, control rate cooling and plunging into liquid nitrogen. Genotypic stability of cryopreserved Euglena gracilis CCAP 1224/5Z Harding et al. (2010) CryoLetters 31,

16 Sustainable Pathways for Algal Bioenergy Post-cryopreservation functional/ phenotypic stability Hédoin et al. (2006) J. appl. Phycol. 18, 1-7.

17 Sustainable Pathways for Algal Bioenergy Post-cryo functional/ phenotypic stability SampleTotal carotenoids (µg/g) Zeaxanthin (%)  - carotene (%) Others (%) A-408 Contol A-408 Post-thaw A-408 Control A-408 Post-thaw

18 Sustainable Pathways for Algal Bioenergy Conclusions Rapid expansion algal cultures Production GMOs on increase Large sums of money being invested Move from biofuels to biotechnology Still needs to be underpinned Recent EnAlgae survey – Demonstrated within some groups how little though is being given to this

19 Sustainable Pathways for Algal Bioenergy KBBE- SeaBioTech Financial support & infrastructure Scientific collaborators Glyn Stacey (NIBSC, UK) Thomas Mock & Rachel Hipkin (UEA, UK) Peter Kroth & Matthias Buhmann (Konstanz, D) Thomas Friedl & Maike Lorenz (SAG, D) Steffi Gäbler-Schwarz (AWI, D) Keith Harding & Erica Benson (DAMAR, UK) Josef Elster & Jaromir Lukavský (IB, CZ) Alena Lukešová (ISB, CZ) Katia Comte & Rosi Rippka (previously IP, F)


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