Presentation on theme: "Douwina BOSSCHER, PhD Manager Nutrition Research ORAFTI Group"— Presentation transcript:
1 Douwina BOSSCHER, PhD Manager Nutrition Research ORAFTI Group Health Benefits of Inulin & Oligofructose with emphasis on Bone Health SYNBIOTIC VIABILITY Nutraceuticals Seminar Food and Drink Innovation Network March , UKDouwina BOSSCHER, PhD Manager Nutrition Research ORAFTI Group
2 Contents Introduction to Pre-, Pro- and Synbiotics Inulin and Oligofructose:2. To enhance Probiotic Viability3. To improve Bone Health
3 Introduction Prebiotics PREbiotics are non-digestible food ingredients that beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon which improve host health.(Gibson et al Nutr. Res. Rev. 17: )Properties that has to be fulfilled before classification:Resistance to gastric acidity, no hydrolysis and/or absorption in gastro-intestinal tract.Fermentation by the intestinal microflora.Selective stimulation of growth and/or activity of a limited number of beneficial intestinal bacteria (associated with health and well-being) (confirmation in randomised, double-blind and placebo-controlled human studies)
4 Inulin and oligofructose (Cichorium Intybus) SALADINULIN SOURCE(by water extraction)75% water17% inulin8% others«WITLOOF»«BELGIAN ENDIVES»(2nd year growth in the dark)COFFEE SUBSTITUTE(by roasting)
5 Natural Extraction Process Partial hydrolysisExtractionInulinOligofructose(hot water)(enzymatic)GFnGFn and FnChicory roots> T/year15-17% inulinß(2-1)DP =ß(2-1)DP = 2 - 8Glucose Fructose Reducing fructoseDP = Degree of Polymerisation
7 Inulin and Oligofructose Prebiotic (bifidogenic) Effect Human volunteer trialsBifidobacteria (Log10/g faeces)15 g 8 g 5 g 20 g 15 g 8 gGibson et al., 1995; Menne et al., 2000; Rao et al., 2001; Tuohy et al., 2001; Kleessen., 1997
8 Probiotics and Synbiotics PRObiotics are live microorganisms, which when administered in adequate amounts confer a health benefit on the host (FAO, 2002).SYNbiotics are a combination of prebiotics and probiotics.
9 Prebiotics, Probiotics and Synbiotics Each gram of fructan ingestedLiving bacteria in the dietOligofructose and/or Inulin in the dietSTOMACHSMALL INTESTINESurvivalCOLONStimulation of beneficial endogenous bacteriaFixation ActivityTotal transfer to the colonStimulates bifidobacterialactobacilliExogenous bacteriaExcretion
10 Health Benefits of Prebiotics and Probiotics (dependent on current research results) * Strain dependent (incl. viability and ability to colonize)ConditionPREBIOTICSPROBIOTICS*Gut Health (bifidogenic) Gut Function (diarrhoea, constipation and pathogen resistance)Immune stimulation (inflammation, allergy)Colonic cancerLactose intoleranceHypocholesterolaemiaHypolipidaemiaCalcium absorption and Bone Health
11 Synbiotic ApproachPrebiotics to enhance viability of probiotic bacteria in foods (e.g. during storage).Prebiotics to enhance survival and viability of probiotic bacteria during gastro-intestinal passage (stomach and small intestine).Prebiotics to enhance colonisation and adherence of probiotic bacteria in the large intestine.Synergistic approach of the health benefits of prebiotics and probiotics (e.g. butyric acid production).Combinations of the health benefits of prebiotics and probiotics.
12 Inulin and oligofructose to Enhance Probiotic Viability Effect of oligofructose on the microflora of an ABT-type fermented milk during refrigerated storageVarga et al Milchwissenschaft 58: 55-58Fermented Acidophilus-Bifidus-Thermophilus (ABT milks)ABT-5 starter cultureWith oligofructose (BeneoTMP95) (1, 3 and 5% w/v) or without (control)Storage at 4°C for 6 wksSurvival (%) by plate counts
13 Effect of oligofructose on the microflora of an ABT-type fermented milk during refrigerated storage Varga, L. et al Milchwissenschaft 58: 55-58bAddition of oligofructose showed a SIGNIFICANTLY BENEFICIAL EFFECT on the SURVIVAL (viability) of BIFIDOBACTERIA (probiotic) when compared to the control (P<0.05). The more oligofructose present, the more pronounced the effect was.
14 Survival and Activity of probiotic Lactobacilli in Skim Milk Containing Prebiotics Desai et al Food Microbiology and Safety 69: 57-60Reconstituted skim milk (12%) with lactobacilli (1x107cfu/ml)With oligofructose (BeneoTMP95) (5% w/v) or without (control)Storage at 4°C for 4 wksViability (%) (by plate counts)VIABILITY of LACTOBACILLI (probiotic) in skimmed milk was SIGNIFICANTLY ENHANCED by the presence of OLIGOFRUCTOSE when compared to the control (P<0.05).
15 ConclusionsThere exist clear beneficial prebiotic-probiotic interactions in finished food productsThe use of prebiotics improves the viability of probiotic bacteria in finished food products
16 Examples of Synbiotic Food Products “… probiotic and prebiotic together form a powerful combination that can help to maintain a healthy digestive system. Probiotic + prebiotic = synbiotic …” “ …one little bottle of probiotic and prebiotic, leave you smiling on the inside.”“.. Synbiotic yogurt contains prebiotic soluble fibre which supports the growth of probiotic Bifidobacteria BB12 and Lactobacillus to strengthen your body’s natural defence system…”“… contains prebiotic active fibre whichsupports the growth of probiotic bifido cultures …”
17 Inulin and Oligofructose to Improve Bone Health Optimization of Calcium Absorption & Bone Formation During Early Puberty
18 Bone Mineral Density During Life Cycle Maximising ‘Peak Bone Mass’ can postpone osteoporosis in later lifeBone Mineral DensityWomen after menopause loose ± 10% of bone mass per decadeDANGER ZONEVertebra at 30 years oldVertebra at 60 years old1020304050607080Years
19 Inulin and oligofructose to Enhance Bone Health A combination of prebiotic short- and long-chain inulin-type fructans enhances calcium absorption and bone mineralization in young adolescents Abrams et al Am. J. Clin. Nutr. 82:Children’s Nutrition Research Center, HoustonRandomized, double-blind, case-control intervention trialHabitual intake mg Ca/dayLong-term intervention study (1y)8 g/d prebiotic (BeneoTMSynergy1) vs. control (maltodextrin) (Ca-fortified orange juice)Dual stable isotopes (46Ca/42Ca) (48-h urine collection)DXA
20 Fructans Chain Length Distribution Enriched Inulin (BeneoTMSynergy1)Degree of Polymerisation1210203060OligofructoseInulin
21 Inulin and Oligofructose Increase Calcium absorption Calcium absorption after 2 mo and 1 y was significantly higher in the Synergy1 group than in the controls (resp. P<0.001 and P < 0.05)
22 Inulin and Oligofructose Improve Bone Mineralization *Bone mineral density (BMD) and bone mineral content (BMC) were significantly higher in the Synergy1 group than in the controls (P < 0.05)
23 ConclusionsAddition of Synergy1 (8 g/d) improved calcium absorption by 20% and the effect was observed during the whole supplementation period (1 y)Long-term supplementation with Synergy1 (8 g/d) improved bone mineral density (BMD) (+ 5%) and bone mineral content (BMC)Long-term results are in accordance with earlier (short-term) observations in adolescent girls (Griffin et al., 2002; 2003) and post-menopausal women (Holloway et al., in press) showing an increased calcium (and magnesium) absorption with Synergy1.