1. SEXUAL HYBRIDIZATION SANITARY ASPECTS GENETIC TRANSFORMATION EMBRYOGENESIS GENETIC CHARACTERISATION G&H WP2 BREEDING SYSTEMS

2. G&H WP2 BREEDING SYSTEMS TWO STEP ORGANOGENESIS ONE STEP ORGANOGENESIS AXILLARY BUDS BASAL PLATES

3. G&H WP2 BREEDING SYSTEMS EMBRYOGENIC CELL SUSPENSIONS P8

4. G&H WP2 BREEDING SYSTEMS Bombarded calli. Hygromycin 25 mg/l

5. G&H WP2 BREEDING SYSTEMS Control. Hygromycin 25 mg/l

6. G&H WP2 BREEDING SYSTEMS GUS activity. Selected calli

7. G&H WP2 BREEDING SYSTEMS Milestones & Deliverables M&D 2000 Milestones: Establishment of a micropropagated collection of garlic clonesdone Axenic material available for transformation experiments done Deliverables:none planned

8. G&H WP2 BREEDING SYSTEMS Milestones & Deliverables M&D 2001 Milestones: Callus lines presenting high levels of transient expression of uidA gene done Highly transformable callus lines available for stable transformation experiments done Regeneration of transgenic plants steadily expressing reporter and selectable genesin progress Garlic transgenic plants availablein progress Deliverables: DP12 paper on reliable transformation of garlicdelayed DP1 DP12c (added DP): paper on new system of garlic regeneration (I) done (2002) DP12d (added DP): paper on new system of garlic regeneration (II) done (2002)

9. G&H WP2 BREEDING SYSTEMS Milestones & Deliverables M&D 2002 Milestones: Regeneration of transgenic plants steadily expressing genes from the sulphur pathwaydelayed transgenic garlic plants with alterations in the sulphur metabolism availabledelayed Deliverables:none

10. G&H WP2 BREEDING SYSTEMS DELIVERABLES -Effect of light in the organogenic ability of garlic roots using a one step in vitro system Martin N., Garrido J., Martín J. & Barandiaran X. (next month in the web) -Effect of Flurprimidol in the organogenic ability of garlic roots using a one step in vitro system Martin N., Garrido J., Martín J. & Barandiaran X. (next month in the web) ADVANCES IN GARLIC TISSUE CULTURE AND TRANSFORMATION. DOCTORAL THESIS. NIEVES MARTIN. NOVEMBER 14 TH 2002.

11. PEOPLE INVOLVED XABIER BARANDIARAN NIEVES MARTÍN JOSÉ GARRIDO JESÚS MARTÍN G&H WP2 BREEDING SYSTEMS