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Boronic acid-modified magnetic materials for antibody purification by Vijaykumar L. Dhadge, Abid Hussain, Ana M. Azevedo, Raquel Aires-Barros, and Ana.

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Presentation on theme: "Boronic acid-modified magnetic materials for antibody purification by Vijaykumar L. Dhadge, Abid Hussain, Ana M. Azevedo, Raquel Aires-Barros, and Ana."— Presentation transcript:

1 Boronic acid-modified magnetic materials for antibody purification by Vijaykumar L. Dhadge, Abid Hussain, Ana M. Azevedo, Raquel Aires-Barros, and Ana C. A. Roque Interface Volume 11(91): February 6, 2014 ©2014 by The Royal Society

2 (a) Modification of dextran-coated MP via the GLYMO route. Vijaykumar L. Dhadge et al. J. R. Soc. Interface 2014;11: ©2014 by The Royal Society

3 (a) Quantity of BSA and IgG bound to the support under different modification stages. Vijaykumar L. Dhadge et al. J. R. Soc. Interface 2014;11: ©2014 by The Royal Society

4 Binding of human IgG at the surface of MP-Dex modified with APBA by GLYMO method MP-BA-3 (a,b) and MP-DX-2 control (c,d). Vijaykumar L. Dhadge et al. J. R. Soc. Interface 2014;11: ©2014 by The Royal Society

5 Magnetic particle characterization by DLS for particle size distribution of MP-Bare, MP-TEOS, dextran (MP-DX-2) and APBA-coated MP (MP-BA-3) (a); zeta potential change of dextran and APBA-coated MP (MP-BA-3) with change of pH (b); TEM image of MP-BA-3 magne... Vijaykumar L. Dhadge et al. J. R. Soc. Interface 2014;11: ©2014 by The Royal Society

6 Electrophoreses gel in denaturation conditions to verify the binding capacity as well as the best elution conditions for IgG from the GLYMO modified APBA-coated MP (MP-BA-3) (a). Vijaykumar L. Dhadge et al. J. R. Soc. Interface 2014;11: ©2014 by The Royal Society


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