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F1 PRESS F1 FOR GUIDEANCE. Haemophilus, Mycobactria, Corynbacteriaua & Bordetella Haemophilus3 Station 1: Gram stain of Haemophilus4 Station 2: Gram stain.

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Presentation on theme: "F1 PRESS F1 FOR GUIDEANCE. Haemophilus, Mycobactria, Corynbacteriaua & Bordetella Haemophilus3 Station 1: Gram stain of Haemophilus4 Station 2: Gram stain."— Presentation transcript:

1 F1 PRESS F1 FOR GUIDEANCE

2 Haemophilus, Mycobactria, Corynbacteriaua & Bordetella Haemophilus3 Station 1: Gram stain of Haemophilus4 Station 2: Gram stain of Haemophilus (pus)5 Station 3: X,V factors & growth6 Station 4: Growth of H.Influenzae on agar7 Station 5: Satellitism 8 Station 6: Treatment & diseases9 Station 7: Summary 10 Mycobacteria11 Station 8: Ziehl-Neelsen stain12 Station 9: Lowenstien Jensen media13 Station 10: Treatment & diseases14 Station 11: Summary15 Cornybacteria16 Station 12: Gram stain of Cornybacteria17 Station 13: Albert stain of Cornybacteria 18 Station 14: Media used19 Station 15: ELEK’s plate20 Station 16: Treatment & diseases21 Station 17: Summary22 Bordetella23 Station 18: Laboratory diagnosis24 Station 19: Tratment & diseases25

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4 Coccobacilli It can look like Bacillus or may look like coccus Station 1

5 Station 2

6 Different species of “Haemophilus” have different requirements to grow and make colonies. Some of them require X & V factors ( Haemophilus influenzae) Some of them need X factor only (Haemophilus ducreyi) Some of them need V factor only (Haemophilus parainfluenzae) X factor = heamatin V factor = Nicotinamide, Adenine & Dineiceotide This Bacteria grow when there is V factor. E.g. (H. Parainfluenzae) This Bacteria grow when there are V & X factors. E.g. (H. Influenzae) Station 3

7 No growth No growth Growth Only X factor X+V factors Station 4

8 X Factor present Staphylococcus strip Producing V Factor H.Influenzae growing here because of presence of both X & V factors This is called Satellitism Station 5

9 Station 6 Haemophilus influenzae Infection:- meningitis acute epiglotitis in children (sore throught) osteomylitis & arthritis Cause bacteremia Treatment:- “Ampicilin” if beta lactamase producers  “Chloramphenicol” Vaccination:- Present by Polysaccharide capsule “serotype b” Haemophilus influenzae Infection:- respiratory tract infections No bacteremia

10 Station 7 Gram stain Type Needs (X,V factors) Grows on Chocolate agar Grows on Blood agar -ve-ve CoccobacilliCoccobacilli X + V V only YesYes NoNo

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12 Station 8

13 Station 9

14 Station 10 Mycobacterium tuberculosis Infection:- 1-TB “lung infection with bloody cough” 2- Meningitis Treatment:- “Pyrazinamide + Isoniazid + Rifampicin”  2 months then, “Isoniazid + Rifampicin”  4 months Vaccination:- B.C.G “Bacille-Calmatte Guerin “10 years protection”

15 Type “of Mycobacterium”: Stain used: Medium used: Acid-Fast Bacilli Ziehl-Neelsen Stain Lowenstein-Jensen medium Station 11

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17 Station 12

18 Station 13

19 There is growth but this is a wrong plate Black color Station 14 Something like this

20 Station 15

21 Station 16 Corynbacterium diphtheriae Infection:- Diphtheria “Bull neck appearance because of pharyngitis” affect: 1- nervous tissue damage (exotoxin) 2- heart muscle damage (exotoxin) 3- laryngeal obstruction 4- affect skin No bacteremia Treatment:- “Penecilin” Vaccination:- Present by toxoid “DPT vaccine” *D= diptheria toxoid *P= pertussis *T= tetanus

22 Type & “gram” stain: Stains used: Media used: What is used for toxogenity test? Gram +ve bacilli Gram & Albert Potassium tellurite & Loeffler’s ELEK’s plate Station 17

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24 Station 18 (coocobacili)

25 Bordetella pertussis Infection:- Whooping cough “due to an endotoxin” usually 1 year of age complications: bronchopneumonia, bronchiatasis & subconjuctival hemorrhage Treatment:- “Erythromycin” but only affective at early stages if beta lactamase producers  “Chloramphenicol” No bacteremia Vaccination:- Present by whole killed bacteria “DPT” Station 19

26 دعواتكم يا شباب

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