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INTEGRATED MEDICINE MICROBIOLOGY. CASE 1 A 44-year-old woman presents to the physician’s office for evaluation of skin growths on her right arm. She reports.

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Presentation on theme: "INTEGRATED MEDICINE MICROBIOLOGY. CASE 1 A 44-year-old woman presents to the physician’s office for evaluation of skin growths on her right arm. She reports."— Presentation transcript:

1 INTEGRATED MEDICINE MICROBIOLOGY

2 CASE 1 A 44-year-old woman presents to the physician’s office for evaluation of skin growths on her right arm. She reports that a few weeks ago she developed some small, red bumps on her right palm, which seemed to come together into a larger nodule. This then ulcerated, but it never was painful. She has been putting topical antibiotic on this area, and it seemed to be improving. However, in the past week she has noticed new growths extending up her forearm that appear just like the original lesion. She denies having skin lesions anywhere else, denies systemic symptoms such as fever, and has no history of anything like this before. She has no significant medical history and takes no medications. She is employed as a florist and floral arranger. On examination, she is comfortable appearing and has normal vital signs. On her right palm you see a circular, 1-cm-diameter, ulcerated area with a surrounding red, raised border. There are two identical appearing, but smaller, lesions on the forearm. Microscopic examination of a biopsy taken from one of the lesions reveals numerous white blood cells and cigar-shaped yeast forms.

3 CASE QUESTION What is the most likely infectious cause of these lesions? ◆ What is the most likely route by which this infection was transmitted?

4 COMPREHENSION QUESTIONS 1. Which of the following fungi is most likely to cause cutaneous disease? A. Aspergillus fumigatus B. Candida albicans C. Cryptococcus neoformans D. Histoplasma capsulatum E. Sporothrix schenckii 2. A woman who pricked her finger while pruning some rose bushes develops a local pustule that progressed to an ulcer. Several nodules then developed along the local lymphatic drainage. The most reliable method to identify the etiologic agent is which of the following? A. Culture of the organism in the laboratory B. Gram stain of smear prepared from the lesion C. India ink preparation D. Skin test for delayed hypersensitivity E. Stain the culture with potassium iodide

5 CASE 2 An 8-year-old boy, a child of immigrants from El Salvador who moved to the southwestern United States 3 months ago, presents to the emergency room with abdominal pain and vomiting. He was ill for a day, but his symptoms have worsened in the past few hours, and his parents panicked when they saw a worm in his vomitus. He has no significant medical history and has taken no medications. On examination, he appears very ill and in obvious pain. His temperature is 37.7°C (99.9°F), his pulse is 110 beats per minute, and his blood pressure is normal. His mucous membranes are dry, but his head and neck exam is otherwise normal. He is tachycardic, and his lungs are clear. His abdomen has high-pitched, tinkling bowel sounds on auscultation and is diffusely tender to palpation. There is, however, no rebound tenderness. An abdominal x-ray shows air-fluid levels consistent with a small bowel obstruction. His parents saved and brought in the worm that he vomited. It is 5 inches long and reddish-yellow in color.

6 CASE QUESTION ◆ What organism is most likely responsible for the patient’s illness? ◆ How did the patient become infected?

7 COMPREHENSION QUESTIONS 1. A definitive diagnosis of ascariasis can be made by observing which of the following? A. An eosinophilia in a differential white blood cell count B. Motile larvae in a stool sample C. Larvae in x-ray of lungs D. An adult worm passed during a bowel movement 2. Ascariasis is most effectively treated with which of the following drugs? A. Mebendazole B. Metronidazole C. Niclosamide D. Praziquantel 3.A person presents to his physician complaining of chronic GI symptoms. A diagnosis of A. lumbricoides is made. Human nematodes infect individuals via different routes. This patient is most likely infected by which of the following? A. Larvae penetrating unprotected skin B. Ingesting larvated eggs C. Eating uncooked pork D. Internal autoinfection

8 CASE 3 A 32-year-old man with known HIV is brought to the hospital with diarrhea. He has had between 15 and 25 watery stools a day for the past 2 weeks. He has had a low grade fever and felt very fatigued, but denies vomiting. He has not passed any blood in his stool. He says that he has lost 8 lb in this time frame. He is on a “triple therapy cocktail” of AZT, 3TC, and a protease inhibitor for his HIV. His last CD4 cell count was 150 cells/mm3. On examination, his temperature is 37.2°C (98.9°F), pulse is 110 beats per minute, blood pressure is 95/75 mm Hg, and respiratory rate is 24 breaths per minute. In general, he appears cachectic. His eyes are dry and sunken. His mucous membranes are moist. His cardiovascular exam is notable for tachycardia, and he has orthoscopic changes on sitting up from lying down. His abdomen has hyperactive bowel sounds, but is soft and only mildly tender. His stool is heme negative. A modified acid-fast stained stool sample reveals multiple red and pink, round oocysts.

9 CASE QUESTION ◆ What is the most likely cause of diarrhea? ◆ How is this infection most commonly acquired?

10 COMPREHENSION QUESTIONS 1] A 33-year-old woman has chronic diarrhea. A fecal sample is obtained. Microscopic identification of which of the following stages of the organism would provide the strongest evidence for cryptosporidiosis? A. Cyst B. Oocyst C. Egg D. Sporocysts E. Merozoites 2] A 24-year-old male scientist is diagnosed with chronic cryptosporidiosis. He asks about the epidemiology of this disorder. Which of the following accurately describes the disease or the etiologic agent? A. Is self-limiting in immunocompromised patients B. Reproduces sexually and asexually in different hosts C. Can be acquired through sporozoites transmitted by an insect vector D. Is transmitted through drinking water contaminated with animal feces E. Is the only human parasite that produces oocysts

11 COMPREHENSION QUESTIONS 3] Chronic, debilitating cryptosporidiosis is most likely to affect which of the following individuals? A. Dairy farmers B. Individuals with AIDS C. Infants placed in day care centers D. Zoo animal handlers E. Hikers who drink from streams and lakes

12 CASE 4 A 53-year-old male farmer presents for evaluation of a growth on his arm. About a week ago, he noticed some mildly itchy red bumps on his arm. They started to blister a day or two later and then ruptured. During this time he had a low-grade fever, but otherwise felt well. Further questioning reveals that he has had no ill contacts and never had anything like this before. He has cows, horses, goats, sheep, and chickens on his farm. On examination of his right upper arm, you find a 4.5-cm circular black eschar surrounded by several vesicles (blisters) and edema. He has tender axillary lymph node enlargement (adenopathy). A Gram stain of fluid drained from a vesicle and a biopsy from the eschar both show chains of gram-positive bacilli on microscopy. ◆ What organism is the likely cause of this disease? ◆ What are the primary virulence factors of this organism?

13 COMPREHENSION QUESTIONS [ 1] A wound specimen obtained from a person working with wool from a Caribbean island demonstrated a large gram-positive rod from a nonhemolytic colony with swirling projections on blood agar. The most likely method to demonstrate spores would be which of the following? A. Acid-fast stain B. Gram stain C. India ink stain D. Malachite green stain 2] Which of the following is the current preferred antimicrobial treatment of cutaneous anthrax? A. Aminoglycosides B. Ciprofloxacin C. Penicillin D. Tetracyclines 3] Bacillus anthracis is unique to other bacteria. It is the only bacteria to possess which of the following? A. An endotoxin B. An exotoxin C. A polypeptide capsule D. A polysaccharide capsule E. Lipopolysaccharide in its outer cell wall F. Teichoic acid in its outer cell wall

14 CASE 5 A 60-year-old man presents to the emergency room with severe abdominal pain. He has had mild, left lower abdominal cramping pain for about 3 days, which has worsened in the past 8 hours. He has also had nausea, fever, and chills. On examination, he is in obvious pain, has a fever of 38.6°C (101.5°F) and has an elevated heart rate (tachycardia). His abdominal examination is notable for absent bowel sounds, diffuse tenderness, and rigidity when palpated. An x-ray reveals the presence of free air in the abdominal cavity. He is taken for emergency surgery and found to have severe diverticulitis with a perforated colon. Cloudy peritoneal fluid is collected. An anaerobic culture grows Bacteroides fragilis. ◆ What characteristics are noted on Gram staining of B. fragilis? ◆ What are its primary mechanisms for resisting phagocytosis?

15 COMPREHENSION QUESTIONS [1] During an emergency surgery, a 60-year-old male is found to have severe peritonitis and a perforated colon. Foul-smelling cloudy peritoneal fluid is collected. Subsequent analysis reveals the growth of black pigmented colonies on Bacteroides bile esculin agar. No growth is detected in the presence of kanamycin, colistin, or vancomycin. Which of the following microorganisms is most likely involved in this case? A. Actinomyces israelii B. Bacteroides fragilis C. Clostridium difficile D. Enterococcus faecalis E. Porphyromonas gingivalis F. Prevotella melaninogenica 2] Which of the following is the treatment of choice to control this infection in this patient (described in Question 1])? A. Cephalothin B. Erythromycin C. Metronidazole D. Penicillin

16 COMPREHENSION QUESTIONS 3. Among the many virulence factors produced, B. fragilis produces an enzyme that allows the organism to survive in the presence of small amounts of oxygen. Which of the enzymes listed below catalyzes the following reaction? 2O−2 + 2H+ → H2O2 + O2 A. β-Lactamase B. Myeloperoxidase C. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase D. NO synthase E. Oxidase F. Superoxide dismutase 4] A foul-smelling specimen was obtained from a 26-year-old woman with a pelvic abscess. Culture grew both aerobic and anaerobic gram-negative bacteria. The most likely organisms are which of the following? A. Actinomyces israelii and Escherichia coli B. Bacteroides fragilis and Listeria monocytogenes C. Bacteroides fragilis and Neisseria gonorrhoeae D. Clostridium perfringens and Bacteroides fragilis E. Escherichia coli and Peptostreptococcus

17 CASE 6 A 28-year-old woman presents to the office for the evaluation of a rash. She had just returned from a weeklong camping trip in the New England area, when she noted the presence of a circular, red rash on her lower abdomen. Also, she has had a low-grade fever, and some achiness and fatigue. Examination of her abdomen reveals a 10-cm flat, red, circular patch with some central clearing. No other skin rashes are noted, and the remainder of the examination is normal. The blood cultures are negative. You make the presumptive diagnosis of erythema migrans and send blood for confirmatory serologic studies. ◆ What organism is the etiologic agent of erythema migrans? ◆ What are the primary reservoir and vector of transmission of this agent?

18 COMPREHENSION QUESTIONS 1] A 9-year-old boy presents with a migratory rash with central clearing on the back of his neck. The child had recently been on vacation with his family in Oregon and had gone hiking. The child’s pediatrician observes the rash and suspects an infection with B. burgdorferi. Which of the following is thought to be a virulence factor of this organism? A. Intracellular growth in leukocytes B. Endotoxin release C. Localization in reticuloendothelial cells D. Antiphagocytic capsular antigen E. Expression of outer surface proteins

19 2] If the child’s infection is left untreated, which of the following symptoms would most likely appear? A. Urethritis B. Centripetal spread of rash C. Biphasic illness with fever and chills D. Stiffness in the knees E. Swelling of lymph nodes 3] A small tick, of the genus Ixodes, most commonly transmits B. burgdorferi. Which of the following diseases is also transmitted by a tick? A. Q fever B. Leptospirosis C. Ehrlichiosis D. Yellow fever E. Eastern equine encephalitis COMPREHENSION QUESTIONS

20 CASE 7 A 19-year-old woman presents for the evaluation of pelvic pain. The pain has progressively worsened over the past week. She has also been having some burning with urination and a vaginal discharge. She is sexually active, has had four lifetime partners, takes oral contraceptive pills, and occasionally uses condoms. On examination, she appears in no acute distress and does not have a fever. Her abdomen is soft with moderate lower abdominal tenderness. On pelvic examination, she is noted to have a yellow cervical discharge and significant cervical motion tenderness. No uterine or adnexal masses are palpated, but mild tenderness is also noted. A Gram stain of the cervical discharge reveals only multiple polymorphonuclear leukocytes. A direct DNA probe test subsequently comes back positive for Chlamydia trachomatis. ◆ How does C. trachomatis enter a target cell? ◆ What are the two stages of the C. trachomatis life cycle?

21 COMPREHENSION QUESTIONS 1] A 32-year-old immigrant woman from Tanzania delivers a healthy baby boy. Because this woman had no regular doctor, no preliminary tests were performed prior to delivery. Thirteen days after delivery, the child develops swelling of both eyes with the presence of a yellow discharge. The presence of intracytoplasmic inclusion bodies is detected. Which antibiotic would be most appropriate in this situation? A. Tetracycline B. Ceftriaxone C. Penicillin G D. Doxycycline E. Erythromycin 2] Which diagnostic test is best to identify an infection with C. trachomatis? A. Aerobic and anaerobic blood cultures B. Stool culture C. DNA probe D. Urine culture E. Culture and darkfield microscopy

22 3] A 29-year-old bird collector presents to the local clinic with what he describes as flu-like symptoms. He doesn’t look ill, and has a slight fever, headache, and a dry hacking cough. He denies the production of sputum or hemoptysis. There are no crackles heard on auscultation, and a radiograph shows small streaks of infiltrate. It is determined that he has pneumonia. What is the most likely organism causing his disease? A. Streptococcus pneumoniae B. Chlamydophila psittaci C. Haemophilus influenzae D. Staphylococcus aureus E. Chlamydophila pneumoniae COMPREHENSION QUESTIONS

23 CASE 8 A 5-year-old girl is brought to the physician’s office because of “pink eye.” She was sent home from kindergarten yesterday by the school nurse because her left eye was red. When she awakened this morning, the right eye was red as well. She has had watery drainage but no purulent discharge. She’s had a mild head cold with a runny nose and a mild sore throat but no fever. When her mother called the school this morning, she was told that five of her daughter’s classmates were out with pink eye today. On examination, the child has injected conjunctiva bilaterally with clear drainage. No crusting of the lashes is noted, and the corneas are clear. She has mildly tender preauricular adenopathy. The remainder of her examination is unremarkable. ◆ What organism is the most likely cause of this infection? ◆ How does this organism gain entry into host cells?

24 COMPREHENSION QUESTIONS 1] An 11-year-old boy attending summer Boy Scout camp develops symptoms of sore throat, headache, fatigue, and conjunctivitis. He is seen by the camp medical staff and on examination is found to have a slight fever of 39.8°C (103.6°F), but no rash. Within the next 1–2 days, several of the other campers develop similar symptoms, which last for 5–7 days. The larger number of campers with similar symptoms indicates that a common source of infection is causing the outbreak. Which of the following activities is the most likely source of the campers’ infection? A. Hiking in wooded areas with tall grass B. Sharing water canteens with other campers C. Sleeping outdoors without protective netting D. Swimming in the camp pond E. Walking barefoot in the bath house

25 COMPREHENSION QUESTIONS 2] The causative agent in the question above was determined to be an adenoviral infection. Which of the following best describes this viral agent? A. Nonenveloped, double-stranded DNA virus with fibers at its vertices B. Nonenveloped, double-stranded, circular DNA virus C. Enveloped, single-stranded, negative-sense RNA virus D. Enveloped, double-stranded, linear DNA virus with glycoprotein spikes E. Enveloped, double-stranded, circular DNA virus 3] A 2-year-old child attending day care develops diarrhea and gastroenteritis as a result of an adenoviral infection. Which of the following adenoviral serotypes would most likely be responsible for this girl’s illness? A. Type 4 B. Type 7 C. Type 19 D. Type 37 E. Type 41

26 CASE 9 You are called to examine a 1-day-old male because the nurse is concerned that he is jaundiced. He was born by spontaneous vaginal delivery to a 19-year-old gravida1 para1 after a full-term, uncomplicated pregnancy. The mother had no illnesses during her pregnancy; she did not use tobacco, alcohol, or drugs; and the only medication that she took was prenatal vitamins. She denied any significant medical history, and there is no family history of genetic syndromes or illnesses among children. The infant is mildly jaundiced but has a notable abnormally small head circumference (microcephaly). His cardiovascular examination is normal. His liver and spleen are enlarged on palpation of the abdomen. Neurologic exam is notable for the lack of a startle response to a loud noise. CT scan of his head reveals intracerebral calcifications. The pediatrician explains to the child’s mother that the virus involved is the most commonly transmitted transplacental viral infection in the United States.

27 CASE QUESTION ◆ What is the most likely cause of this infant’s condition? ◆ How did he likely acquire this? ◆ What is the test of choice to confirm the diagnosis?

28 COMPREHENSION QUESTIONS 1] An 18-year-old female presents to her physician with a 1-week history of fever, sore throat, fatigue, and myalgia. Physical examination reveals enlarged tonsils and exudative pharyngitis. Based on her clinical presentation, her physician diagnoses her with infectious mononucleosis. Because there are multiple causes of infectious mononucleosis-like illnesses, which of the following diagnostic assays would rule out CMV as the causative agent of this patient’s infection? A. A negative Gram stain of a throat swab B. A lack of atypical lymphocytes in the patient’s blood C. A positive histological finding of cytomegaly D. A positive Monospot test

29 COMPREHENSION QUESTIONS 2] A previously healthy 8-year-old boy develops a classic childhood illness as a result of a primary viral infection. Which of the following agents would most likely produce symptomatic disease in a boy of this age? A. Cytomegalovirus B. Epstein-Barr virus (EBV) C. Herpes simplex virus 2 (HSV-2) D. Poliovirus E. Varicella-zoster virus (VZV) 3] A 32-year-old HIV infected male is noted to have acute CMV infection causing acute gastrointestinal symptoms. The treating physician has ordered that antiviral therapy be administered. Which of the following is most likely to be targeted by the antiviral agent? A. Protease cleavage B. Nuclear transport of virus C. Synthesis of viral DNA D. Transcription of viral proteins E. Viral-cell fusion

30 CASE 10 A 17-year-old female is brought to the physician’s office for evaluation of a sore throat and fever. Her symptoms started approximately 1 week ago and have been worsening. She has been extremely fatigued and has spent most of the last 3 days in bed. She denies any ill contacts. She has no significant medical history, takes no medications, and has no allergies. On examination, she is tired and ill appearing. Her temperature is 38.5°C (101.3°F). Examination of her pharynx shows her tonsils to be markedly enlarged, almost touching in the midline. They are erythematous and covered with white exudates. She has prominent cervical adenopathy, which is mildly tender. A cardiovascular examination is normal, and her abdomen is soft, nontender, and without palpable organomegaly. A rapid streptococcal antigen test in the office is negative. You send a throat culture and decide to start amoxicillin for strep pharyngitis, assuming that the office test was a false-negative. Two days later, you get a call from her mother stating that she has had an allergic reaction to the amoxicillin, and she now has a red rash from head to toe.

31 CASE QUESTION ◆ What is the most likely diagnosis of this patient? ◆ What is the most likely cause of her infection? ◆ In what human cells can this virus replicate? In what cells can it cause latent infection?

32 COMPREHENSION QUESTIONS 1] Which of the following statement regarding the serologic diagnosis of infectious mononucleosis is correct? A. A heterophile antibody is formed that reacts with the membrane protein of EBV. B. A heterophile antibody is formed that agglutinates sheep or horse red blood cells. C. A heterophile antigen occurs that cross-reacts with atypical lymphocytes. D. A heterophile antigen occurs following infection with both EBV and CMV. 2] A transplant patient taking high levels of immunosuppressive drugs becomes infected with EBV and develops a lymphoma. The dosage of immunosuppressive drugs given to the patient is subsequently decreased, and the tumor regresses. Which of the following properties of EBV infection is related to the patient’s tumor development? A. Immortalization of B cells B. Increased white blood cell count C. Presence of atypical lymphocytes D. Production of heterophile antibodies

33 COMPREHENSION QUESTIONS 3] A 21-year-old man visits the student health center suffering from a sore throat, swollen glands, fatigue, and a temperature of 39.4°C (103°F). Examination of the patient’s peripheral blood smear shows 10 percent atypical lymphocytes, an elevated white blood cell count, and a positive heterophile antibody test. The patient asks for antimicrobial therapy. Which of the following statements would best dictate the clinician’s response? A. α-Interferon is helpful in EBV infections but has multiple side effects. B. Ribavirin is effective in patients over 60 years. C. Attenuated-viral vaccine has been developed but not effective in this case because the infection has already occurred. D. There is no effective treatment.


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