Handling of Surgical Specimen 1.Fresh tissue - avoid crushing - keep in refrigerator at 4 C - avoid wrapping with dry gauze - wrap in gauze soaked with normal saline - put in plastic bag, glass or metal containers and seal
Handling of Surgical Specimen 2. Fixed specimen - fixation in fixative (10% buffered formalin, alcohol, Zenker solution, B-5 solution etc.) - put the specimen in glass, plastic or metal containers with fixative and seal - put the gauze on top in case of floating specimen - volume of fixative: 10 times of the specimen - rate of fixation 1mm/hour
Clinical Pathology Specimen 1.Blood 2.Serum or plasma 3.Urine 4.Fluid from cavities (pleural, pericardial, peritoneal, cerebrospinal-CSF, vitreous or aqueous humor, joint, any cavity)
Laboratory Investigation in Dermatopathology 1. Special stains for skin smear - Acid fast bacilli stain (AFB) - Gram’s stain - etc.
Laboratory Investigation in Dermatopathology 2. KOH preparation (20%) 3. Skin smear for dark field examination for syphilis 4. Skin scraping for scabies
Laboratory Investigation in Dermatopathology 5. Tzanck smear for diagnosis of herpetic infection or bullous disease - for diagnosis of herpetic infection or bullous disease - new vesicle, open the roof with sharp blade - scrape at vesicle base - air dried - stain with Wright or Giemsa stains