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The Well- Connected Cell Text and image sources are included using the notes function of this file.

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Presentation on theme: "The Well- Connected Cell Text and image sources are included using the notes function of this file."— Presentation transcript:

1 The Well- Connected Cell Text and image sources are included using the notes function of this file

2 Classes of cell-cell junctions typefunction tight junctionssealing anchoring junctionsstructural interconnection communicating junctions communication

3 Tight (Occluding) Junctions ZO-1 ZO-3 DNA Claudins Occludins JAMs Organizers Informants Sealers ß-catenin PTEN

4 Tight Junctions seal dense tracer

5 Tight Junction interactome

6 Anchoring junctions strengthen

7 I. Belt (Adherens) Junctions in cardiac muscle Cadherin CAMs

8 Belt Junctions back up tight junctions

9 The Adherens Junction Interactome The Adherens Junction interactome

10 Forging links GFP::actin

11 II. Desmosomal Junctions Cytokeratin Desmosomes (Plakoglobins anchor MFs)

12 A day in the life of an epithelium Desmocollin -GFP Imaged over a 24 hour period

13 III. Hemidesmosomes anchor IMF to ECM via integrin Heterodimers (18 alpha x 8 beta) ECM RGD (Arg-Gly-Asp)

14 Loss of hemidesmosomal connections creates blistering diseases

15 IV. Focal Adhesion sites (FAds) link microfilaments to ECM

16 FAd interactome

17 Focal Adhesion Kinase (FAK) /VASp

18 FAds anchor stress fibers Actin-GFP and Vinculin

19 GFP::VASp localizes at FAds

20 Special Delivery? Microtubules target FAds GFP::tubulin and Ds-Red::zyxin FAds) GFP::CLIP-170 (+TIP) and Ds-Red::zyxin FAds)

21 Communicating Junctions GFP-Connexin-43 localizes at cell-cell contact points

22 Connexin hexamers form gap junctions fields 2nm

23 Gap junction fields Tightly- packed gap junctions

24 Gap junctions permit low MW molecules (<1kDa) to move between cells scrape

25 Gap junction interactome

26 AFM of gap junction field reveals the 6- fold symmetry of the connexion channel +Ca ++ -Ca ++ And a role for calcium in conductance regulation

27 Calcium Wave

28 Electrical coupling in engineered heart muscle tissue

29 Plasmodesmata The symplast refers to the linked protoplasmic network in plants

30 Plant cell lineages are linked in distinct symplastic routes


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