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Jessica Huska Cory Weiss Jessie Bennett. What? Determine the Calcium Concentration in Different Types of Milk and Compare to One Another – 2% Milk – Almond.

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Presentation on theme: "Jessica Huska Cory Weiss Jessie Bennett. What? Determine the Calcium Concentration in Different Types of Milk and Compare to One Another – 2% Milk – Almond."— Presentation transcript:

1 Jessica Huska Cory Weiss Jessie Bennett

2 What? Determine the Calcium Concentration in Different Types of Milk and Compare to One Another – 2% Milk – Almond Milk – Soy Milk – Rice Milk

3 Importance Most Abundant Mineral in the Body – About 1-2% of the body weight – Stored in the Bones and Teeth – Used for: vascular contraction and vasodilation muscle function nerve transmission intracellular signaling hormonal secretion

4 Ways to Get Calcium Through a well rounded diet – Milk – Yogurt – Cheese – Broccoli – Kale – Soft Bone Fish Dietary Supplements – Multivitamin – Carbonate – Citrate

5 Recommended Daily Amount Recommended Daily Amount It differs for the individual based on their age

6 Too Little or Too Much Too Little – No short term effects seen – Long term: Osteopenia, Osteoporosis, Bone Fractures – Symptoms of deficiency: numbness and tingling in the fingers, convulsions, and abnormal heart rhythms (most extreme) Too Much – Causes Constipation – Lowers the absorption of iron and zinc – Risk of Kidney Stones (adults) – Current studies show that having too much calcium can also contribute to prostate cancer and heart disease **more research being done

7 Upper Limit Upper Limit Can’t get close with just diet alone

8 Groups at Risk of Calcium Inadequacy Postmenopausal Women Amenorrheic Women Female Athlete Triad Lactose Intolerance Vegetarians

9 Method Standardization of EDTA – Dissolve 3.6 g of EDTA in 16 mL of ammonium buffer in 1000 mL volumetric flask – Dissolve 0.5 g of Calcium carbonate in 100 mL of 0.1 M HCL – Titrate 3 good trials Calcium content in milk - Mix 2-7 mL of milk solution, buffer, and indicator - Titrate 3 good trials with EDTA

10 Method cont. Spike tests - added 0.1 g of calcium carbonate to samples and titrated with EDTA Frozen samples - froze 30 mL of each milk sample and titrated with EDTA

11 Proof of Method We standardized a solution of EDTA - Must be at pH of 10 - The calcium in milk reacts quantitatively with EDTA and forms a stable complex - Calmagite indicator changes color from magenta to blue at a pH of >7.0 - Indicator changes color when last of Ca2+ is complexed by EDTA - the ammonium buffer maintains the pH to stay at 10 We used EDTA because in experiment 6 it proved to be successful with finding concentrations of calcium carbonate in a solution

12 Spike Test

13 Calcium Contained

14 Comparisons

15 Percent Difference

16 Daily Value Comparison

17 Calcium in Frozen Milk

18 Comparisons

19 Percent Difference

20 Problems That Occurred The end point was expected to be less than 20 mL of EDTA based on the standardization – The first titration worked with Rice Milk – The indicator wasn’t changing even after mL of EDTA – We tried using different indicators, but still didn’t see a change and the end point – Used a spike to try and figure out the problem, but still we saw no change

21 Solution Changed the Parameters – Initially we were adding 55 mL of milk with enough ammonia buffer to have pH 10 – Realized this was too much  too much calcium in the solution to react – Changed the experiment to use 2 mL of milk plus 5 mL of buffer **except for Rice Milk (7 mL, 10 mL) – Titrations worked successfully

22 A Better Experiment? Instead of comparing refrigerated milks to a frozen sample, we would want to freeze a total of eight or so samples and keep each in for a longer amount of time This way we could have made a calibration curve of Concentration vs Time We could have done this, but didn’t think of it in time

23 In Conclusion Almond milk is the best source of calcium Rice and Soy are poor sources of calcium The only milk seriously effected by being frozen is almond milk

24 References The George Mateljan Foundation. Calcium. The George Mateljan Foundation Web Accessed. National Institute of Health. Dietary Supplement Fact Sheet: Calcium. USA.gov Web Accessed. National Institute of Health.Calcium. USA.gov Web Accessed


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