Presentation on theme: "More Non-linear Microscopy. E x = E xo cos(2 ft) = E xo cos ( t) P x = aE xo cos ( t) + dE 2 xo cos 2 ( t) P = aE + dE 2 + d'E 3 +... Nonlinear."— Presentation transcript:
Only forward direction for objects axially longer than wavelength Dipole Emission Patterns for scattering particles Xie J. Phys. Chem Many dipoles aligned: All forward directed
Mertz, BJ 2001 Calculations show that SHG emitted in two lobes
WT OI/OI Fw Bw Fw Bw Forward/Backward Fractions SHG in Tendon: Wild type vs OI WT larger fibers, also brighter, F/B Ratio?
image plane A 050100150200250 0 10 20 30 40 50 60 Percent Scattered Back Thickness (µm) B Thick tissues, initial photon is multiply scatttered Some goes backwards direction Like SHG Backscattered collection Red, Black: experimental and Monte Carlo simulations From intralipid phantom Blue=mouse Real Tissue: Only Scattering Matters
100 m 50 m 100 m 20 m 30 m deep 70 m deep 100 m deep 0 m deep ABC D E F 50 m CorneacytesSebaceous glands Adipocytes Evans and Xie PNAS CARS Image of Hairless Mouse Ear