Presentation is loading. Please wait.

Presentation is loading. Please wait.

เทคนิคการใช้งานปิ เปตอย่างถูกต้อง “Guide to Pipetting” 694 ซ. รัชดานิเวศน์ 24 ถ. ประชาราษฎร์บำเพ็ญ เขตห้วย ขวาง กรุงเทพฯ 10321 Tel. 0-2274-2456 (automatic.

Similar presentations


Presentation on theme: "เทคนิคการใช้งานปิ เปตอย่างถูกต้อง “Guide to Pipetting” 694 ซ. รัชดานิเวศน์ 24 ถ. ประชาราษฎร์บำเพ็ญ เขตห้วย ขวาง กรุงเทพฯ 10321 Tel. 0-2274-2456 (automatic."— Presentation transcript:

1

2 เทคนิคการใช้งานปิ เปตอย่างถูกต้อง “Guide to Pipetting” 694 ซ. รัชดานิเวศน์ 24 ถ. ประชาราษฎร์บำเพ็ญ เขตห้วย ขวาง กรุงเทพฯ Tel (automatic 9 lines) Fax

3 Air – displacement Positive – displacement

4 Two families for Gilson pipettes Air-displacementPositive-displacement

5 Operating principles Air - displacementPositive - displacement

6 Select the best pipetting system/sample Air-displacement Positive-displacement

7 Air-displacement Forward Mode Reverse Mode

8 Rest position First stop Second stop or purge Air-displacement pipettes forward pipetting mode

9 Rest position First stop Second stop or purge Air-displacement pipettes reverse pipetting mode

10 Positive-displacement Capillary Pistons (CP)

11 Positive-displacement 1/ Preparation2/ Aspiration 3/ Distribution 4/ Ejection Rest position First stop Ejection

12

13

14 Pipetting cycle 1.Fit a tip 2.Set the volume 3.Pre-rinse 4.Aspirate 5.Dispense 6.Eject 7.Hold the pipette

15 Fit a tip multichannels Single channels

16 Set the volume - using the thumbwheel or the push-button Electronic display avoids parallax error For Pipetman Classic use the horizontal position and place your eye in front of the volumeter

17 Pre-rinse Aspirate with the tip and dispense back Why? –Wettability of the tip  Greater uniformity  Greater accuracy and precision When? –After changing the tip –After increasing the volume

18 Aspirate

19 Dispense

20 Eject

21 Hold the pipette

22 Improve your working day Get organized Get organized  Necessary objects within easy arm's reach  Pipettes within easy reach, on a stand  Most frequently used objects in front of you  Waste receptacles no higher than the end of your pipette Posture Posture  Chair or stool so that elbow on the bench  Hands below shoulder height  Reduce the height of applications such as gel loading  Arms close to the body

23 Posture Good Seated posture: Lower back supported by chair Upper back and neck upright Upper arm vertical Elbow bent at 90° Wrist in the same plane as the forearm Standing posture: Lower back and trunk upright Upper back and neck upright Upper arm vertical Elbow bent at 90° Forearm parallel to the floor Wrist in the same plane as the forearm Wrist posture: Forearm parallel to the floor Wrist and forearm in the same plane  Bad Seated posture: Shoulders elevated Upper arm elevated Elbow extended Wrist in deviation Standing posture: Upper back and neck stopped Lower back and trunk stopped Elbow flexed Wrist posture: Upper arm flexed Elbow extended Wrist deviated downward

24

25 Leak test From 1000 µL to 10 mL From 0.2 µL up to 200 µL Step 1Step 2Step 1

26 Cleaning External External Remove the tip ejector Wipe with soap solution Wipe with distilled water Tip ejector Connecting nut Seal & O-ring Piston assembly Internal Internal Disassemble the lower part Clean then rinse the tip holder, piston assembly and seals Leave to air dry and reassemble

27 Disassemble & Reassemble

28 Autoclaving lower part is autoclavable connecting nut; tip ejector; shaft/tip holder Moist heat/121°C/20 minutes/1 bar

29 Maintenance frequency

30

31 Specifications

32 Performances Accurate and precise Systematic error Accurate but not precise Precise but not accurate Random error

33 Errors Random error Random error Tips Technique Piston Systematic error Systematic error Z factor not adequate Needs adjustment

34 E NVIRONMENT E QUIPMENT T ECHNIQUE T IPS Parameters AIR TEMPERATURE WATER TEMPERATURE AIR CONDITIONNING AIR PRESSURE DENSITY DRAUGHTS STABILITY DIRECT SUN HUMIDITY WATER Air buoyancy Repeatability MARBLE TABLE Weighing vessel BALANCE SPECIFICATIONS MAINTENANCE READIBILITY CLASS LINEARITY CONTROLS RECORDER SIZE Shape LID PERIODICAL CHECKS STANDARD MASS Drift Vibrations SPEED OPERATING MODE QUALIFICATION CONSISTENCY EVAPORATION IMMERSION DEPTH PRE RINSING Stabilization Regularity Smooth and regular WETTABILITY Perfectly formed AIR-TIGHT FIT TRACEABILITY Fine point finish Clean

35 Sources of error

36 Excess plastic Imperfect point aperture Rough surface Pipette Tips Identification: mold and cavity Level marks Sealing ring for airtight fit Translucent, no dyes Fully autoclavable, even the filter Fine point and flexibility  Perfect point Non Gilson Tips Gilson Diamond Tips

37 calibration method

38 Test Volume: minimum and Maximum Test Volume: minimum Medium and Maximum Harikul Calibration Laboratory Gravimetry method

39 Equipment : Balances requirement

40 Environmental factors to be respected 101kPa101kPa 22.5 ±7.5°C °C Above 50% 40% to 60% ISO 8655 Harikul Calibration Laboratory Do not forget: Do not forget: Conversion factor Evaporation loss

41

42 Questions / Comments Costumer Service


Download ppt "เทคนิคการใช้งานปิ เปตอย่างถูกต้อง “Guide to Pipetting” 694 ซ. รัชดานิเวศน์ 24 ถ. ประชาราษฎร์บำเพ็ญ เขตห้วย ขวาง กรุงเทพฯ 10321 Tel. 0-2274-2456 (automatic."

Similar presentations


Ads by Google