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A Comparative Phytochemical and Physicochemical Evaluation: Tikta-anguna, Kiri-anguna and Jeewanti Sujatha Hewageegana Menuka Arawwawala Anurakumara Tissera.

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Presentation on theme: "A Comparative Phytochemical and Physicochemical Evaluation: Tikta-anguna, Kiri-anguna and Jeewanti Sujatha Hewageegana Menuka Arawwawala Anurakumara Tissera."— Presentation transcript:

1 A Comparative Phytochemical and Physicochemical Evaluation: Tikta-anguna, Kiri-anguna and Jeewanti Sujatha Hewageegana Menuka Arawwawala Anurakumara Tissera Sami Ariyawansa Induragare Dammaratana 1

2 Introduction Objectives Materials and Methods Results Conclusion 2

3 Tikta-anguna and Kiri-anguna (Behet anguna) Both come under one botanical name - Wattakaka volubilis (Linn.f) Family: Asclepiadaceae Both creepers are large twining shrubs, with long glabrous branches, Leaves thin, ovate 1. Bitter variety 2. Sweet variety Introduction 3

4 Wattakaka volubilis Kiri anguna (Behet anguna)Tikta anguna 4

5 Parts Used: Leaves, roots and tender stalks are used Actions and common uses: Leaves are used for boils and abscesses as an application Roots and tender stalks are used for expectorant properties in Ayurveda and traditional medicine Lactogenic Aphrodisiac Sweet variety –cooling and Tonic 5

6 Jeewanti Leptadenia reticulate (Retz) Wight and Arn. 6 Family : Asclepiadaceae A much - branched twining shrub, branches numerous and younger ones glabrous, Leaves thin, ovate

7 Parts Used: Both roots and leaves Actions and common uses: Both roots and leaves - Skin infections, wounds Leaves - cough, as a respiratory stimulant, Lactogenic Aphrodisiac Cooling and Tonic L. reticulate 7

8 Research background : Ayurvedic texts contain lot of simple and effective as well as both externally and internally used medicinal preparations, containing Jeewanti (L. reticulata) Jeewanti is found in most parts of India, but it is not available in Sri Lanka We had to find a most suitable substitute for Jeewanti 8

9 According to literature survey and the conversation with Senior Scientist in charge, Haldummulla Research Gardens, we were able to find morphologically, nearly similar two plants (Tikta-anguna and Kiri-anguna) for Jeewanti In addition, they have similar practices for disease conditions 9

10 Comparison of the Two plants L. reticulate and W. volubilis L. reticulateW. volubilis FamilyAsclepiadaceae Classical namesJivanti, Svarnajivanti, Tittajivanti Hemajivanti, Sujivanti, Svarnajivantika MorphologyMany similarities Common uses Skin ailments Respiratory tract infections ENT disorders Lactogenic Burning sensation Antipyretic Use for poisoning Aphrodisiac Increase urination Skin ailments Respiratory tract infections ENT disorders Lactogenic Burning sensation Antipyretic Use for poisoning Aphrodisiac Increase urination 10

11 Purpose of this study was to investigate the possibility of using Tikta-anguna and Kiri- anguna as substitutes for Jeewanti 11

12 Objectives  To compare the phytochemical parameters of W. volubilis (Tikta-anguna and Kiri-anguna) and L. reticulata (Jeewanti)  To compare the physicochemical parameters of W. volubilis (Tikta-anguna and Kiri-anguna) and L. reticulata (Jeewanti)  To compare the fingerprint profiles of W. volubilis (Tikta-anguna and Kiri-anguna) and L. reticulata (Jeewanti) 12

13 Materials and Methods W. volubilis (both varieties) were collected from Colombo, Sri Lanka Authentication was done by Scientist, Botany Division, BMARI, Navinna, Maharagama Voucher specimen No : W 2561 L. reticulata was collected from Jamnagar, India Authentication was done by Botanist, Vidyaratnam Foundation (Research and Development Section), Thrissur, Kerala, India, according to the standards of Ayurveda Pharmacopeia in India 13

14 Plant materials Plant materials (leaves with tender stalk) were shade dried and cut into small pieces Then prepared the powder using the grinder Materials were kept in air tight containers until used 14

15 Screening of Phytochemical compounds  Presence /Absence of Phenolic compounds Flavanoids Tannins Coumarins Alkaloids Saponins Steroid glycosides 15

16 Determination of physicochemical parameters P hysicochemical parameters were evaluated according to WHO standards 16

17 Hot Water Extract (HWE) Hot Ethanol Extract (HEE) Cold Water Extract (CWE) Cold Ethanol Extract (CEE) % Extractable mater = Weight of extract x 100 Weight of sample 17 Extractable matter in

18 Ash Content : 1. % Total ash = Ash Weight x 100 Weight of sample 2. % Acid-insoluble ash = Acid insoluble Ash Weight x 100 Weight of sample 3. % Water-soluble ash = Total Ash Weight - water insoluble residue in total ash x 100 Weight of sample 18

19 TLC fingerprint profiles of W. volubilis and L. reticulata Extract - methanol extract Plant material - 4.0 g Extracts were spotted on a silica gel plate and TLC fingerprints were developed using Dichloromethane: Ethyl acetate: Cyclohexane (40:1:10 v/v/v) Vanillin-sulphuric acid reagent was used for colour development 19

20 Statistical analysis Data were analyzed by using Mann Whitney test and findings of p <0.05 was considered to indicate statistical significance All data were presented as mean ± SEM All the values were express as dry weight of the sample and they were performed in triplicate for accuracy 20

21 Table 1 : Phytochemical classes of W. volubilis varities and L. reticulata Phytochemical Classes Presence or Absence of Phytochemicals Classes Tikta-angunaKiri-anguna (Behet-anguna) Jeewanti (HWE) (HEE)(CWE) (CEE) (HWE)(HEE)(CWE) (CEE) (HWE)(HEE)(CWE) (CEE) Phenolic compounds  Saponins  -  -  -  -  -  - Flavanoids  ---  ------- Steroid glycoside s  Tannins  Coumarin  --  --  Alkaloids-  ---  ---  -- 21 Results

22 22 Screening of phytochemical compounds of (a) cold and hot ethanol and water extracts of W. volubilis and L. reticulata revealed the presence of phenolic compounds, tannins, and s teroids (b) cold and hot water extracts of W. volubilis and L. reticulata revealed the presence of saponins (c) cold and hot ethanol and water extracts of L. reticulata and hot extracts of W. volubilis revealed the presence of coumarin (d) In addition, hot water extract of W. volubilis contained flavanoids

23 Physico-chemical parameters W. Volubilis (%) - (Dry wt basis) L. Reticulata (%) (Dry wt basis) (Jeewanti) Tikta angunaKiri anguna ( Behet anguna ) Hot water extractable matter 37.37± 0.5631.67 ± 0.3331.54 ± 0.46 Hot ethanol extractable matter 19.62± 0.103.36 ± 0.0513.19 ±0.42* Cold water extractable matter 7.99± 0.047.01± 0.285.99 ± 0.49 Cold ethanol extractable matter 1.55± 0.021.37 ± 0.056.34± 0.13* Total ash content 11.80± 0.0716.46± 0.1116.63 ± 0.30* Acid-insoluble ash Content 0.91± 0.020.81± 0.012.30 ± 0.02* Water-soluble ash Content 5.76± 0.216.11 ± 0.066.13 ± 0.03 Table 2: Physicochemical parameters of Wattakaka volubilis and Leptadenia reticulata 23

24 Following three physicochemical parameters of Jeewanti were significantly different from that of Tikta-anguna and Kiri-anguna 1. Hot and Cold Ethanol Extractable matter 2. Total ash Content 3. Acid-insoluble ash Content 24

25 1.L. reticulata (Jeewanti ) 2. W. volubilis (Kiri-anguna) 3. W. volubilis (Tikta-anguna ) 1 2 3 2µL 2µL 2µL 1 2 3 2µL 2µL 2µL Wave length – at 254 nm After spraying Vanillin -sulphuric acid 3. TLC fingerprint profiles of Tikta-anguna, Kiri-anguna and Jeewanti 25

26 Red – Tikta anguna (W. volubilis) Blue – Kiri anguna (W. volubilis) Green – Jeewanti (L. reticulata) TLC- densitogram fingerprint of Tikta-anguna, Kiri-anguna and Jeewanti, at 254 nm 26

27 According to TLC fingerprints of Tikta-anguna, Kiri-anguna and Jeewanti, similar compounds were identified in three samples 27

28 According to the results of this study, phytochemical parameters, physicochemical parameters and fingerprint profiles of Tikta- anguna and Kiri-anguna have very similar properties and they are similar to Jeewanti also 28

29 conclusions As this study reveals, Tikta-anguna, Kiri-anguna and Jeewanti have more similarities in terms of phytochemical parameters, physicochemical parameters and fingerprint profiles Therefore, both Tikta-anguna and Kiri-anguna can be used as substitutes for Jeewanti in ayurvedic preparations 29

30 According to traditional medicine, Kiri-anguna (Behet-anguna) is commonly used for medicinal preparations Further, Kiri-anguna (behet-anguna) can be considered as the “sweet variety” of W. volubilis and it has similar properties such as GUNA (Quality), RASA (Taste), VIPAK (Metabolism), VIRYA (Potency), PRABHAV (Impact) to Jeewanti After considering all, we can conclude that kiri-anguna (behet- anguna) is much better to use as a substitute for Jeewanti 30

31 References Parangepe, P., Indian medicinal Plants, 2005, Chaukhamba Sanskrit Pratishthan, Delhi.pp 116-117 Jayaweera, D.M.A., Medicinal Plants (Indigenous and Exotic) Used in Ceylon, 2006, The National Science Foundation, Sri Lanka, pp 225 Kirtikar, K. R., Basu, B. D., Indian Medicinal Plants- volume III, 1996,Valley offset printers and publishers, Dehra Dun, pp 2267-2268 Senaratna, L. K., A Check List of Flowering Plants of Sri Lanka, 2001, The National Science Foundation, Sri Lanka, pp 46 Sharma, P.C., Yelne, M.B., Dennis, T.J., Database on Medicinal plants used in Ayurveda, 2002, Central Council for Research in Ayurveda and Siddha, New Delhi. Pp 270-272 31

32 Acknowledgement Mr Piyal Marasingha – Senior scientist, Dept of Ayurveda Dr. Sudeesh Kumar and the Vidyaratnam Foundation (Research and Development Section), Thrissur, Kerala, India Industrial Technology Institute National Centre for Advanced Studies for Humanities and Social Sciences (NCAS) 32

33 33

34 » JeewantiTikta-angunaKiri-anguna (Bitter verity)(Sweet verity) Rasa Madhura Katu, TiktaMadhura GunaLaghu, SnigdhaRuksha, Laghu Laghu, …… VeeryasheetaUshnaSheeta Vipaka Madhura KatuMadhura Prabhawa Jivniya


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