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Control Mechanism of the Circadian Clock for Timing of Cell Division in Vivo Science, Vol. 302, Issue 5643, 255-259, October 10, 2003 Takuya Matsuo, 1,2*

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Presentation on theme: "Control Mechanism of the Circadian Clock for Timing of Cell Division in Vivo Science, Vol. 302, Issue 5643, 255-259, October 10, 2003 Takuya Matsuo, 1,2*"— Presentation transcript:

1 Control Mechanism of the Circadian Clock for Timing of Cell Division in Vivo Science, Vol. 302, Issue 5643, 255-259, October 10, 2003 Takuya Matsuo, 1,2* Shun Yamaguchi, 1* Shigeru Mitsui, 1 Aki Emi, 1 Fukuko Shimoda, 1 Hitoshi Okamura 1 *

2 O ritmo circadiano afeta o timing da divisão celular? Como? Modelo: retirada de 2/3 do fígado do camundongo – efeito do ciclo circadiano na regeneração. ZT0: hepatectomia no 1 o instante de luz ZT8: hepatectomia no após 8 horas de luz

3 Fase S: 36 horas: pico de síntese de DNA Fase M: ZT8: pico 40 horas ZTO: pico 48 horas

4

5 wee1

6 Camundongos Cry-deficientes Efeitos na regeneração.

7 Qual seria o mecanismo molecular que impede a progressão da fase S para a M?

8

9 CLOCK: regulador positivo de wee 1 CRY: regulador negativo de wee1

10 P Bcdc2 B B MPF inativo MPF ativo

11 Regulação direta de wee1 pelo relógio circadiano. luciferase E-box CLOCKBMAL1 CRY 1,2 Promotor wee1 WEE1 Bcdc2

12


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