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Published byKyle Ward Modified over 4 years ago

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8 seqs/day 96 seqs/2 hrs Bioinformatics for Genomics

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TAGAGCATCGATCGATGCTGCAGATGATGCTAGCATCGGCTAGGCGACG ATCTCGTAGCTA ATCTCGTAGCTAGCTACGACGTCTA ATCTCGTAGCTAGCTA ATCTCGTAGCTAG ATCTCGTAGCTAGC ATCTCGTAGCTAGCT ATCTCGTAGCTAGCTAC ATCTCGTAGCTAGCTACG ATCTCGTAGCTAGCTACGA ATCTCGTAGCTAGCTACGAC ATCTCGTAGCTAGCTACGACG ATCTCGTAGCTAGCTACGACGT ATCTCGTAGCTAGCTACGACGTC ATCTCGTAGCTAGCTACGACGTCT ATCTCGTAGCT A G C T A C G A C G T C T A

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20 30 10 Random base calling at the beggining or the end of read (Phred < 10) Trimming (trim or trim_alt algorithms) Phred does the base calling chromatogram acgatctcgctagctgctactgtagccgcgattattcgcgatctacgtatatcgcgatcgatc Each base has assigned a chance of failure 1% = 0,01 = 10 -2 = Phred 20 Base calling & trimming

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Start End

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Goal: To document the presence of transcripts in a transcriptome [otorrin... in portuguese] EST = Expressed Sequence Tag Partial sequencing of transcripts in EST genome projects actgatcatctcgctgatgcgatc work

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UNIT 2: SOLVING EQUATIONS AND INEQUALITIES SOLVE EACH OF THE FOLLOWING EQUATIONS FOR y. # 1. - 2 x + 5 y = 15 + 2 x 5 y = 2 x + 15 55 y = 2 x + 15 5 y.

UNIT 2: SOLVING EQUATIONS AND INEQUALITIES SOLVE EACH OF THE FOLLOWING EQUATIONS FOR y. # 1. - 2 x + 5 y = 15 + 2 x 5 y = 2 x + 15 55 y = 2 x + 15 5 y.

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