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STARBIND I In vitro. Starbind | In vitroSlide 2 Evaluating binders in vitro: example Solution of toxins 10 µg/l Afl + 2 g/l binder  equilibrium Measure.

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Presentation on theme: "STARBIND I In vitro. Starbind | In vitroSlide 2 Evaluating binders in vitro: example Solution of toxins 10 µg/l Afl + 2 g/l binder  equilibrium Measure."— Presentation transcript:

1 STARBIND I In vitro

2 Starbind | In vitroSlide 2 Evaluating binders in vitro: example Solution of toxins 10 µg/l Afl + 2 g/l binder  equilibrium Measure conc. free toxin( ) at equilibrium in solution  2 µg/l Afl free Afl total : 10 µg/l Afl free : 2 µg/l  Afl bound : 8 µg/l Afl  adsorption: 80% Toxin + binder  complex (pH-traject) 

3 Starbind | In vitroSlide 3 Clay minerals - 2003 Zearalenone 2000 mg/metric ton feed Clay minerals5 kg/metric ton feed Adsorption of 1040 mg ZEA/MT feed =52% pH 2.5 pH 8.0 Starbind Organic acids and salts Yeast product Clay minerals Clinoptilolite

4 Starbind | In vitroSlide 4 Clay minerals & yeast - 2004 Aflatoxin 340 mg/metric ton feed Ochratoxin A 1000 mg/metric ton feed Clay min.& yeast5 kg/metric ton feed pH 3.0 pH 6.5 Starbind Organic acids and salts Yeast product Clay minerals Clinoptilolite Adsorption of 230 mg aflatoxin/MT feed = 67% 302 mg ochratoxin A/MT feed = 30%

5 Starbind | In vitroSlide 5 Clinoptilolite - 2006 Aflatoxin 400 mg/metric ton feed Zearalenone2000 mg/metric ton feed Clinoptilolite10 kg/metric ton feed Adsorption of 398 mg aflatoxin/MT feed = 99% 250 mg zearalenone/MT feed = 12.5% pH 3.0 pH 6.5 Starbind Organic acids and salts Yeast product Clay minerals Clinoptilolite

6 Starbind | In vitroSlide 6 Starbind in commercial feed Kasetsat University Thailand, 2008Starbind Organic acids and salts Yeast product Clay minerals Clinoptilolite Starbind 1kg/MT feed Aflatoxine diff. contamin Fumonisine. diff. contamin. pH 6.5 (3h) Adsorption of Aflatoxine ca 95% Fumonisine. ca 63%.

7 Starbind | In vitroSlide 7 Screening project 2009 ProductMycotoxinpHDosage Starbind Binder F Binder M ZEA, OTA, AFL, at 5, 15, 50, 150 and 500 ppb 3 & 6,52 kg / Ton

8 Starbind | In vitroSlide 8 Screening project 2009 - ZEA Percentage bound contamination (µg/L)

9 Starbind | In vitroSlide 9 Screening project 2009 - OTA Percentage bound contamination (µg/L)

10 Starbind | In vitroSlide 10 Screening project 2009 - AFL Percentage bound contamination (µg/L)

11 Starbind I In vitro – adsorption isotherm

12 Starbind | In vitroSlide 12 Adsorption isotherm: example Solution of toxins 10 µg/l AF + 2 g/l binder  equilibrium Measurement of free toxin( ) at equilibrium In solution  2 µg/l AF free AF total : 10 µg/l AF free : 2 µg/l  AF bound : 8 µg/l AF  Per gram binder: 4 µg AF adsorption  Repeatment with the same dosage of toxin binder at higher concentration of toxin Toxin + binder  complex (pH-traject) 

13 Starbind | In vitroSlide 13 0 4 8 12 16 20 24 28 0 102030405060708090 100110120130140150160170180190200 Contamination [µg/kg] Bound toxin [µg/g binder ] Interpretation of adsorption-isotherm 80% 44% 33% 16%12% 80% 70% 32% 24% 12%9% 90% The steeper the slope  the bigger the affinity to the toxin The higher the saturation line  the higher the max. binding capacity

14 Starbind | In vitroSlide 14 Isotherm voor Starbind: Zearalenone 0-5-10-50-150-500 ppb Starbind2 kg/MT feed Binder G2 kg/MT feed Isotherm pH 3.0 (1h) pH 6.5 (3h) Clay minerals ClinoptiloliteStarbind Organic acids and salts Yeast product pH 3.0 (1h)

15 Starbind | In vitroSlide 15 ZEA binding Adsorption isotherm: Conclusion:  The Starbind isotherm shows a steeper slope  higher affinity to ZEA  Binder G shows a saturating slope 0 50 100 150 200 250 300 050100150200250300350400450500 free zearalenone [ppb] bound zearalenone [ppb] Binder StarbindBinder G

16 Starbind | In vitroSlide 16 ZEA binding Conclusion: Starbind can bind more ZEA Starbind can reduce a higher contamination (120 ppb) to a safe level than binder G 0 50 100 150 200 250 300 350 400 450 500 050100150200250300350400450500550 contamination [ppb] Free zearalenone [ppb] Binder StarbindBinder G


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