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1.Genes não Corinebacterineae Específicos de C. pseudotuberculosis e espécies próximas evolutivamente (Cd) Transferidos de outros clados.

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Presentation on theme: "1.Genes não Corinebacterineae Específicos de C. pseudotuberculosis e espécies próximas evolutivamente (Cd) Transferidos de outros clados."— Presentation transcript:

1 1.Genes não Corinebacterineae Específicos de C. pseudotuberculosis e espécies próximas evolutivamente (Cd) Transferidos de outros clados

2 txid85007[Orgn] AND chromosome[Replicon Type] AND complete[PROP] AND reference[PROP] 110,052 seqs 26 genomes: Corynebacterium (7) Mycobacterium (17) Rhodococcus (1) Nocardia (1) tBLASTn CpCDS 317 NH-CpCDS tBLASTn Other txid NH-CpCDS RGMG tBLASTn UniprotKB + nr 30 CpCDS 26 distinct C. pseudotuberculosis: SMase D Serine protease precursor 280 “no hit”

3 YP_ putative CAAX amino terminal protease family protein COG1807 YP_ hypothetical protein ABC0212 NP_ regulatory protein (padR family) YP_ similar to superfamily I DNA and RNA helicases and helicase subunits YP_ conserved phage-associated protein YP_ conserved phage-associated protein YP_ hypothetical phage-associated protein YP_ PTS system, fructose-specific IIBC component YP_ protein of unknown function DUF181 YP_ hypothetical protein Strop_2434 YP_ Lantibiotic dehydratase domain protein YP_ hypothetical protein Strop_2432 ZP_ Secreted subtilisin-like peptidase YP_ CAAX amino terminal protease family protein NP_ nitrite reductase periplasmic cytochrome c552 YP_ putative cytochrome c nitrite reductase, small subunit NrfH XP_ viral A-type inclusion protein, putative COG gb|AAA serine proteinase precursor** YP_ hypothetical protein MUL_3536 COG ZP_ transcriptional antiterminator, BglG ZP_ nucleoside transporter YP_ similar to superfamily I DNA and RNA helicases and helicase subunits ZP_ transcriptional antiterminator, BglG ZP_ hypothetical protein ACTODO_01808 ZP_ hypothetical protein ACTODO_01815 ZP_ hypothetical protein ACTODO_01815 ZP_ hypothetical protein ACTODO_01815 ZP_ Secreted subtilisin-like peptidase ZP_ hypothetical protein XfasaDRAFT_1247 ZP_ hypothetical protein SpneT_ De onde eles vieram?

4 HitOccurence in other clades

5 Arthrobacter sp. Bacillus clausii KSM-K16 Clostridium tetani E88 Saccharopolyspora erythraea NRRL 2338 Propionibacterium acnes Exiguobacterium sp. Salinispora tropica CNB-440 Salinispora tropica CNB-441 Salinispora tropica CNB-442 Salinispora tropica CNB-443 Idiomarina baltica OS145 Bacillus cereus E33L Bdellovibrio bacteriovorus HD100 Myxococcus xanthus DK 1622 Trichomonas vaginalis G3 Enterococcus faecalis V583 Mycobacterium ulcerans Agy99 Thermosinus carboxydivorans Bacillus sp. NRRL B Saccharopolyspora erythraea NRRL 2338 Thermosinus carboxydivorans Actinomyces odontolyticus ATCC Idiomarina baltica OS145 Xylella fastidiosa Dixon Streptococcus pneumoniae Pular esse slide

6 2.Homologia com baixa similaridade Caso da SMase Matrizes PSSM para COG Matrizes para novos clusters Seed Linkage

7 (psi)tblastn PSSM 114, hits PSSM not BLOSUM tBLASTn nr 48 hits PSSM not “nr” COG No hit COG Ex: Cp-V COG0419 Cp-V COG1178 common 4 hits: Not Corinebacterineae COG annotated Tx85007 Clusters

8 Cp-Draft- V COG0697-Permeases of the drug/metabolite transporter (DMT) superfamilyNostoc sp. COG0697Cyanobacteria Cp-Draft- V COG0488- ATPase components of ABC transporters with duplicated ATPase domainsListeria COG0488Firmicutes Proteobacteria Cp-Draft- V COG1191- DNA-directed RNA polymerase specialized sigma subunitTreponema pallidum COG1191Spirochaetes Cp-Draft- V COG1994-Zn-dependent proteasesNostoc sp. COG1994Cyanobacteria From 280 putative exclusive Cp-CDS, four are found by search using the PSSM matrices, but are no-hits to nr, uniprot and cog:


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