1. 1.Genes não Corinebacterineae Específicos de C. pseudotuberculosis e espécies próximas evolutivamente (Cd) Transferidos de outros clados

2. txid85007[Orgn] AND chromosome[Replicon Type] AND complete[PROP] AND reference[PROP] 110,052 seqs 26 genomes: Corynebacterium (7) Mycobacterium (17) Rhodococcus (1) Nocardia (1) tBLASTn CpCDS 317 NH-CpCDS tBLASTn Other txid85007 310 NH-CpCDS RGMG tBLASTn UniprotKB + nr 30 CpCDS 26 distinct C. pseudotuberculosis: SMase D Serine protease precursor 280 “no hit”

3. YP_946759.1putative CAAX amino terminal protease family protein COG1807 YP_173716.1hypothetical protein ABC0212 NP_781294.1regulatory protein (padR family) YP_001107303.1similar to superfamily I DNA and RNA helicases and helicase subunits YP_056299.1conserved phage-associated protein YP_056299.1conserved phage-associated protein YP_056300.1hypothetical phage-associated protein YP_001813295.1PTS system, fructose-specific IIBC component YP_001159253.1protein of unknown function DUF181 YP_001159259.1hypothetical protein Strop_2434 YP_001537420.1Lantibiotic dehydratase domain protein YP_001159257.1hypothetical protein Strop_2432 ZP_01042386.1Secreted subtilisin-like peptidase YP_084726.1CAAX amino terminal protease family protein NP_969614.1nitrite reductase periplasmic cytochrome c552 YP_630432.1putative cytochrome c nitrite reductase, small subunit NrfH XP_001319569.1viral A-type inclusion protein, putative COG gb|AAA67924.1serine proteinase precursor** YP_907156.1hypothetical protein MUL_3536 COG ZP_01665177.1transcriptional antiterminator, BglG ZP_01170419.1nucleoside transporter YP_001107303.1similar to superfamily I DNA and RNA helicases and helicase subunits ZP_01665177.1transcriptional antiterminator, BglG ZP_02044925.1hypothetical protein ACTODO_01808 ZP_02044932.1hypothetical protein ACTODO_01815 ZP_02044932.1hypothetical protein ACTODO_01815 ZP_02044932.1hypothetical protein ACTODO_01815 ZP_01042386.1Secreted subtilisin-like peptidase ZP_00651843.1hypothetical protein XfasaDRAFT_1247 ZP_01407840.1hypothetical protein SpneT_02001734 De onde eles vieram?

4. HitOccurence in other clades

5. Arthrobacter sp. Bacillus clausii KSM-K16 Clostridium tetani E88 Saccharopolyspora erythraea NRRL 2338 Propionibacterium acnes Exiguobacterium sp. Salinispora tropica CNB-440 Salinispora tropica CNB-441 Salinispora tropica CNB-442 Salinispora tropica CNB-443 Idiomarina baltica OS145 Bacillus cereus E33L Bdellovibrio bacteriovorus HD100 Myxococcus xanthus DK 1622 Trichomonas vaginalis G3 Enterococcus faecalis V583 Mycobacterium ulcerans Agy99 Thermosinus carboxydivorans Bacillus sp. NRRL B-14911 Saccharopolyspora erythraea NRRL 2338 Thermosinus carboxydivorans Actinomyces odontolyticus ATCC Idiomarina baltica OS145 Xylella fastidiosa Dixon Streptococcus pneumoniae Pular esse slide

6. 2.Homologia com baixa similaridade Caso da SMase Matrizes PSSM para COG Matrizes para novos clusters Seed Linkage

7. (psi)tblastn PSSM 114,320 86 hits PSSM not BLOSUM tBLASTn nr 48 hits PSSM not “nr” COG No hit COG Ex: Cp-V1.6830 COG0419 Cp-V1.2590 COG1178 common 4 hits: Not Corinebacterineae COG annotated Tx85007 Clusters

8. Cp-Draft- V1.0010 COG0697-Permeases of the drug/metabolite transporter (DMT) superfamilyNostoc sp. COG0697Cyanobacteria Cp-Draft- V1.8610 COG0488- ATPase components of ABC transporters with duplicated ATPase domainsListeria COG0488Firmicutes Proteobacteria Cp-Draft- V1.15550 COG1191- DNA-directed RNA polymerase specialized sigma subunitTreponema pallidum COG1191Spirochaetes Cp-Draft- V1.17210 COG1994-Zn-dependent proteasesNostoc sp. COG1994Cyanobacteria From 280 putative exclusive Cp-CDS, four are found by search using the PSSM matrices, but are no-hits to nr, uniprot and cog: