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GeneLocation (bp ) PMA1 UAS 1 2 3 4 5 6 7 8 9 -926 ~ -677 -304 ~ -47 168 ~ 376 584 ~ 807 1010 ~ 1250 2018 ~ 2290 2866 ~ 3098 3287 ~ 3500 3448 ~ 3662 3619 ~ 3905 ADH1 UAS 1 2 3 -670 ~ -455 -235 ~ -13 844 ~ 1013 1231 ~ 1400 PYK1 UAS 1 2 3 4 5 -815 ~ -566 -288 ~ 19 508 ~ 799 1104 ~ 1372 1470 ~ 1720 1695 ~ 1995 5 6 7 9 Poly(A) 4 8 32 1 PMA1 (2.7 kb) TATA UAS +1 2 3 Poly(A) ADH1 (1.0 kb) 1 UAS TATA +1 1 4 5 2 3 Poly(A) PYK1 (1.5 kb) TATA UAS +1
Rap1 TBP Rpb1 INPUT * * * * PMA1ADH1 PYK1 123457 1 2 312345 UUU
Spt16 Spt5 Rtf1 INPUT Sub2 Rna14 * PMA1ADH1 PYK1 123457 1 2 312345 UUU PMA1ADH1PYK1 123457 1 2 312345 U U U INPUT * * * *
Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)
Promoter structure and gene regulation. Bacterial Promoters Source:
Chapter 21 (part 1) Transcription. Central Dogma.
Eukaryotic Transcription. Eukaryotic Transcriptional Transcription control is the most important mode of control in eukaryotes. Three RNA polymerases:
4 and 6 October, 2006 Chapter 12 Mechanism of Transcription.
A B Figure S1 pRS425pRS425-FCP1 Rpb3p ChIP efficiency (relative to wt promoter) C Ser2P / Rpb3p ChIP ratio (relative to wt promoter)
HOW DO CELLS KNOW WHEN TO EXPRESS A GENE? DO NOW:.
1 Transcription in eukaryotes RNA Polymerases Promoters General Transcription Factors.
Introduction to Molecular Cell Biology Transcription Regulation Dr. Fridoon Jawad Ahmad HEC Foreign Professor King Edward Medical University Visiting Professor.
M4 RNA Pol II genes: promoters and enhancers -----RNA Polymerase II (RNA Pol II) is located in the nucleoplasm. It is responsible for the transcription.
Snurps? describe how cells modify RNA after transcription.
Cell and Molecular Biology Behrouz Mahmoudi DNA Transcription 1.
Gene expression DNA RNA Protein DNA RNA Protein Replication Transcription Translation Degradation Initiation Elongation Processing Export Initiation.
Assembly of RNA polymerase II preinitiation complex Heather Drilling.
NYKRCP004 RT- PCR report PCR Condition Temp.TimeCyclesFinal Reaction vol. 45 ℃ 30min1 50ul 95 ℃ 15min 1 95 ℃ 30sec ℃ 30sec 72 ℃ 40sec.
Chapter 17 Transcription and Translation From Gene to Protein.
Part 3 Gene expression ( Ⅰ ) transcription Http ： //web.umr.edu/.../ 331figures.htmlweb.umr.edu/.../ 331figures.html.
Ch 10. Multiple forms of Eukaryotic RNA Polymerase.
TATA box Promoter-proximal elements Effects of mutations in promoter element sequences on transcription.
Design review Sampling cell – Buffer - Comparator Ps_TDC v2.0 Herve Grabas - University of Chicago.
Section M: Transcription in EukaryotesYang Xu, College of Life Sciences Section M Transcription in Eukaryotes M1 The three RNA polmerases: Characterization.
M1 The three RNA polymerases: characterization and function M2 RNA Pol Ⅰ genes: the ribisomal repeat M3 RNA Pol Ⅲ genes: 5S and tRNA transcription M4 RNA.
Shine-Dalgarno Motif Ribosome binding site located about 13 bases upstream of AUG start codon SD sequence is: 5’-AGGAGGU-3’ Middle GGAG is more highly.
ZHANG ZHIZHUO MAY 2010 Nucleosome Positioning & Transcription Factor Identification.
Initiation of rDNA transcription Two transcription factors, UBF and SL1, bind cooperatively to the rDNA promoter and recruit RNA polymerase I to form an.
Transcription(II) 王之仰. The DNA control elements in eukaryotic genomes that bind transcription factor often are located much farther from the promoter.
Transcription vs Translation. Central Dogma Transcription Translation.
DNA to Protein – 12 Part one AP Biology. What is a Gene? A gene is a sequence of DNA that contains the information or the code for a protein or an RNA.
RNA Metabolism Transcription - process by which DNA (genetic info) gets made into RNA (mRNA, rRNA, or tRNA) by an RNA polymerase mRNA - messenger RNA -
©2001 Timothy G. Standish Psalm 102:25 25In the beginning you laid the foundations of the earth, and the heavens are the work of your hands.
©2001 Timothy G. Standish Psalm 102:24 25In the beginning you laid the foundations of the earth, and the heavens are the work of your hands.
Transcription AHMP Objectives: 1. Describe the general process of DNA transcription 2. Discuss the function of RNA polymerases 3. Differentiate.
The SV40 Promoter Enhancer GC boxes (6) TATA box.
DNA Rep & Protein Syn – Griffith Transformation Experiment.
Protein Synthesis. Transcription DNA mRNA Occurs in the nucleus Translation mRNA tRNA AA Occurs at the ribosome.
Compiler-Based Register Name Adjustment for Low-Power Embedded Processors Discussion by Garo Bournoutian.
Program.-(4)* Write a program for input two integer number with message and display their sum. Algorithm steps Algorithm steps 1.Display message for input.
Chapt. 10 Eukaryotic RNA Polymerases and their Promoters Student learning outcomes : Explain composition of 3 different nuclear RNAPs; emphasis on pol.
TRANSCRIPTION (DNA → mRNA). Fig. 17-7a-2 Promoter Transcription unit DNA Start point RNA polymerase Initiation RNA transcript 5 5 Unwound.
Chap. 7 Transcriptional Control of Gene Expression (Part B) Topics RNA Polymerase II Promoters and General Transcription Factors Regulatory Sequences in.
Blank Template. Office of Research Services Template Heading Sub text in line form.
M1 The three RNA polymerases: characterization M1 The three RNA polymerases: characterization and function and function M2 RNA Pol Ⅰ genes: the ribisomal.
Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.
Activities of RNA Polymerase sequence specific DNA binding -promoters melts DNA to reveal the template strand selects ribonucleotide (not deoxynucleotides)
Protein Synthesis. One Gene – One Enzyme Protein Synthesis.
945 Kb Kb 1,900 Kb 1,640 Kb 1,120 / 1,100 Kb 815 Kb 450 Kb 915 Kb 680 Kb 555 Kb 610 Kb
Math – What is a Function? 1. 2 input output function.
©2000 Timothy G. Standish Hebrews 1:1-2 1God, who at sundry times and in divers manners spake in time past unto the fathers by the prophets, 2Hath in these.
Decoding the Gene. The Genetic Code is contained in a three- letter sequence called a codon. A codon consists of three consecutive nucleotides, which.
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