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The Relationship of Genus Bacillus, Sulfate Reducing Bacteria and Sulfur Oxidation Bacteria in the Activated Sludge Sui Kanazawa Advanced Environmental.

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Presentation on theme: "The Relationship of Genus Bacillus, Sulfate Reducing Bacteria and Sulfur Oxidation Bacteria in the Activated Sludge Sui Kanazawa Advanced Environmental."— Presentation transcript:

1 The Relationship of Genus Bacillus, Sulfate Reducing Bacteria and Sulfur Oxidation Bacteria in the Activated Sludge Sui Kanazawa Advanced Environmental Engineering Course Gunma National College of Technology Sui Kanazawa Advanced Environmental Engineering Course Gunma National College of Technology 2012 Japan-Taiwan Symposium on intelligent Green and Orange (iGO) Technology

2 2/12 1. Background the corrosion of sewer pipe, B.Subtilis, Sulfate Reducing and Sulfur Oxidation bacteria (SRB and SOB) 2. Purpose of this research 3. Materials and Method 4. Result and Discussion 5.Summary 1. Presentation Outline

3 3/12 Corrosion of sewer pipe due to sulfuric acid Corrosion of sewer pipe due to sulfuric acid Lifespan : 50 years only 10~20 years Protection : the sheet covering method △ unworkable 1. Back ground (1/3)

4 the corrosion of concrete Ca(OH)₂ + H₂SO₄ →CaSO₄+2H₂O the corrosion of concrete Ca(OH)₂ + H₂SO₄ →CaSO₄+2H₂O 4/12 The mechanism of the corrosion due to SRB The mechanism of the corrosion due to SRB 1. Back ground (2/3) H₂OH₂O SO ₄ ² ⁻ H₂SH₂S H₂SH₂S the occurrence of H₂S by SRB SO₄²⁻ + 2C + 2H₂O→2HCO₃+H₂S the occurrence of H₂S by SRB SO₄²⁻ + 2C + 2H₂O→2HCO₃+H₂S the diffusion of H₂S the formation of sulfuric acid H₂S + 2H₂O→H₂SO₄ +4H₂ by SOB H₂S + 2H₂O→H₂SO₄ +4H₂ the formation of sulfuric acid H₂S + 2H₂O→H₂SO₄ +4H₂ by SOB H₂S + 2H₂O→H₂SO₄ +4H₂ SRB : Sulfate Reducing Bacteria SOB : Sulfur Oxidation Bacteria

5 rod-shaped, gram-positive bacteria exists widely in nature 5/12 1. Back ground (3/3) the inhibition of sewer odor the limitation of activity of Escherichia coli limitation of SRB?

6 6/12 2. Purpose To prove the interaction between B. subtilis, SRB and SOB To prove the interaction between B. subtilis, SRB and SOB To develop the technique for corrosion protection of concrete

7 3. Materials and Method (1/4) 7/12 the activated sludge the activated sludge the night soil treatment plant in Gunma membrane separation method the activated sludge of a B. subtilis-dominated wet sample freeze-dried sample a biological floc composed of bacteria

8 3. Materials and Method (2/4) 8/12 Measurement of the Bacillus. subtilis Measurement of the Bacillus. subtilis dilution at 10³ to10 ⁷ times cultivation for 2 days

9 3. Materials and Method (3/4) 9/10 Measurement of the SRB Measurement of the SRB cultivation for 10 days MPN method

10 3. Materials and Method (4/4) 10/12 Measurement of the SOB Measurement of the SOB Starkey medium shaking culture for a week MPN method

11 6. Result and discussion 11/12 The number of the colonies The number of the colonies [1] data of the night soil treatment plant [2] data of municipal wastewater disposal plant the number of SRB is not small in the activated sludge of a B. subtilis- dominated the sludge was odorless The activity of SRB was inhibited by some factor ?

12 7. Summary 12/12 The number of SRB was not small especially in the activated sludge of B. subtilis-dominated. The number of SRB was not small especially in the activated sludge of B. subtilis-dominated. We need to research to examine others waste water treatment plant to examine others waste water treatment plant to examine the activated sludge of laboratory scale reactor to examine the activated sludge of laboratory scale reactor We need to research to examine others waste water treatment plant to examine others waste water treatment plant to examine the activated sludge of laboratory scale reactor to examine the activated sludge of laboratory scale reactor


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