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Kevin A. Negrete - President DNA Workshop (Bologna, Italy)

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Presentation on theme: "Kevin A. Negrete - President DNA Workshop (Bologna, Italy)"— Presentation transcript:

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2 Kevin A. Negrete - President

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4 DNA Workshop (Bologna, Italy)

5 To increase the quality of life after 40 To live better… longer

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7 Public Health Nutrition Two main factors contributing longer (historical) lifespan

8 Environmental (controllable) Cellular Oxidation Oxidative Stress (Free Radicals) Genetic (non-controllable) Preprogrammed Methyl Group Loss Preprogrammed shortening of Telomere length Two main factors contributing to limited lifespan

9 the formation of nascent oxygen (O 2 ) and the elimination of free radicals (O+) reduce cellular oxidation

10 Environmental (controllable) Cellular Oxidation Oxidative Stress (Free Radicals) Genetic (non-controllable) Preprogrammed Methyl Group Loss Preprogrammed shortening of Telomere length Two main factors contributing to limited lifespan X X ? or is it? ?

11 Research has allowed scientists to identify central aging system in cells – and specifically the aging clock in DNA The two basic DNA markers that regulate aging are Telomere length and Methyl Group Loss

12 ConceptionBirthDeath 15,000 Base Pairs 10,000 Base pairs1,500 Base Pairs Inside the center or nucleus of a cell, our genes are located on twisted, double-stranded molecules of DNA called chromosomes. At the ends of the chromosomes are stretches of DNA called telomeres, which protect our genetic data, make it possible for cells to divide. Telomeres have been compared with the plastic tips on shoelaces because they prevent chromosome ends from fraying and sticking to each other, which would scramble an organism's genetic information to cause cancer, other degenerative diseases, or death. Each time a cell divides the telomeres get shorter (by BP). Healthy cells only divide between times. When telomeres get too short, the cells no longer can divide and becomes inactive, sustain genetic damage and ultimately die. Telomere

13 Similar to telomere shortening – the loss of Methyl Groups (CH3) shows a specific preprogrammed degradation throughout mammalian lifespan. The degradation is accelerated by oxidative stress, poor nutrition, lack of exercise, and environmental toxins. H H H H H H H H H

14 Birth 10% loss 25 yrs 20% loss 50 yrs 30% loss 75 yrs 40% loss Death Final Common Pathway of Aging – DNA Demethylation

15 Understand true anti-aging (DNA) mammalian chemistry consists of 2 goals: elongate telomers and increase DNA methylation. ENHANCED NUCLEOTIDE SUPPLEMENATION

16 Longevity of Control vs. Treated Animals ControlTreated 2500

17 Record mammalian longevity achieved through weekly injection of DNA and RNA Study began with 750 day old rats with usual longevity of 800 to 900 days Identical conditions except treated animals received nucleic acid injections Post 8 weeks control rats lost fur and vitality, whereas treated animals increased muscle mass, libido, and activity levels Control animals all died in less than 150 days from start of study Treated animals lived a minimum of 850 up to 1500 additional days from start of study, doubling and even tripling the usual life span

18 Understand true anti-aging (DNA) mammalian chemistry consists of 2 goals: elongate telomers and increase DNA methylation. HOMOCYSTEINE

19 What is Homocysteine? Homocysteine is a toxic amino acid by-product produced by our own organism and accumulates in the bloodstream. Elevated homocysteine is not only the indicator of, but is the CAUSE of many major diseases includin Alzheimer’, Arthritis, Cancer, Chronic Fatigue, Depression, Diabetes, Heart Attacks, Infertility, Obesity, Strokes, Thyroid Problems, and Ulcers Homocysteine also accelerates the oxidation of LDL

20 Homocysteine Level VS Cardiac Risk Ratio

21 Research has shown that higher HOMOCYSTEINE levels are directly related to demethylation and telomere shortening 3 ways to detoxify HOMOCYSTEINE

22 Methionine B12 Folic Acid Homocysteine (Hcy) Zn Vitamin B6 Cysteine Glutathione ATP S-adenosyl-methionine (SAMe) Donates CH 3 to: DNA Proteins Lipids Carbohydrates Myelin Detox Pathways Neurotransmitter Production And others S-adenosyl- homocystei ne (SAH) Betaine (TMG) Choline DMG Methyl Group Transfer Metabolic Pathways

23 We have developed THE ENGINE OF REMETHYLATION making cells younger through Enhanced Nucleotide Supplementation PurinesPyrimidines DNAAdenineCytosine GuanineThymine RNAAdenineCytosine GuanineUracil

24 Methionine B12 Folic Acid Homocysteine (Hcy) Zn Vitamin B6 Cysteine Glutathione ATP S-adenosyl-methionine (SAMe) Donates CH 3 to: DNA Proteins Lipids Carbohydrates Myelin Detox Pathways Neurotransmitter Production And others S-adenosyl- homocystei ne (SAH) Betaine (TMG) Choline DMG Methyl Group Transfer Metabolic Pathways

25 Could life-extension and anti-aging be as simple as supplying the body with DNA and RNA? PurinesPyrimidines DNAAdenineCytosine GuanineThymine RNAAdenineCytosine GuanineUracil

26 Primary source of nucleotides is endogenous synthesis from amino acids and simple precursors Therefore nucleic acids have generally been considered nonessential nutrients Under stress, however, some tissues unable to produce enough nucleotides to support tissue needs In these conditions, nucleic acids bases may become essential nutrients for optimum tissue repair and function Glycine, Serine, Aspartic Acid, Glutamine

27 Nucleic Acids : Food & Supplementation

28 So why doesn’t everyone just take a giant nucleic acid pill?

29 Orally ingested nucleic acids undergo intensive metabolic degradation in upper GI tract Over 99% of purine bases oxidized to uric acid before entering portal circulation About 95% of pyrimidines are metabolized before leaving intestinal lining Only 3% of ingested pyrimidines reach liver

30 Photo Acoustic Resonance Laser

31 Laser Enhancement Technology Nutrient molecules get distorted during food or supplementation processing. Result: reduced nutrient assimilation by the cells. Laser Enhancement Technology reshapes nutrient molecules. Result: increased nutrient assimilation by the cells.

32 Control Betaine HCl Laser Homogenized Betaine HCl Crystal

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34 Extensive experimental and clinical evidence for benefit from nucleotide supplementation Increased survival in mice with Staph aureus bacteremia from 21% to 71% Significantly increased survival in mice with Candida sepsis – greater effect with parenteral nucleotides than oral administration Survival in animals with high dose radiation exposure increased from 5% to 50%

35 Single injection of RNA in mice post tumor immunization improved outcome from 0% to 40% long term survival Rate of liver regeneration in rats post 70% hepatectomy significantly greater if IV nucleic acid bases given In young rats with chronic diarrhea, appearance and enzyme function of intestine greatly improved Improved growth and maturation in young animals

36 Infants given formula supplemented with nucleotides to mimic content of breast milk showed more benevolent intestinal flora an less diarrhea (50 times more nucleic acids in breast milk vs. formula which is significant in developing countries) Supplemented infants had better lipid profiles with higher HDL cholesterol Significant improvement in human cellular immune function Significant improvement in memory in animals and humans Accelerated wound healing

37 ATP delivery specifically associated with numerous benefits Improved pulmonary function, even in cystic fibrosis Enhanced cardiac function – strengthening the heart Relief of nerve pain and improved nerve conduction Direct antitumor effects (RNA nucleotide) specifically incubation with ATP breast and prostate cancer

38 Synergistic antitumor effects in conjunction with chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell types IV ATP has halted progression of tumor cachexia (advanced cancer with rapid weight loss) 50 mcg/kg/minute x 24 hours x 3 weeks Protection of tissues from radiation injury Increased natural killer cell function Survival of patients in ICU for shock (multi-organ failure) increased from 70% to 100%

39 Current research Enhanced Nucleotide Supplementation

40 Reducing Homocysteine reduces the risk of death from the top 5 killer diseases: Heart Attackby 80% Strokesby 82% Cancerby 33% Diabetesby ??% Alzheimer'sby 50%

41 Homocysteine Levels (units = micromoles/litre) Below 6.3 = Very Low Risk = Low Risk = High Risk = Very High Risk Every 5 point decrease in homocysteine level = 50% reduced risk of cardiovascular death 26% reduced risk of cancer death

42 Methylation Formula Study Dose Response Curve Homocysteine Level- Treatment Group (n=22) Active Supplementation Group Months P=.00001

43 Methylation Formula Study Dose Response Curve Homocysteine Level- Placebo Group (n=11 ) P= NS

44 Methylation Formula Study Dose Response Curve Homocysteine Level- Treatment Group for subjects with Baseline Homocysteine Level > 10 (n=7) Active Supplementation Group Months P=.009 P=.001 P=.00001

45 Population study completed in Western Norway with over 18,000 subjects – Hordaland Study Directly established a relation between homocysteine levels and chronological aging Homocysteine level of >13 was associated with someone 65 years old Homocysteine level of <7 was associated with someone 25 years old

46 CD4 Count 600/ul200/ul10/ul Viral Load 0/ml50,000/ml201,000/ml CD4 vs. T-Cells 30%-60%10%1.17% Normal AIDS Brian Bryan Maphalala - AIDS Victim

47 Before taking CELLFOOD and CELLFOOD DNA-RNA CD4 Count: 10 units; Viral Load: 201,000 After taking CELLFOOD & CELLFOOD DNA-RNA for 6 months Mrs. Winnie Mandela congratulated him for his work with HIV and AIDS people

48 Does it really work?

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