Presentation on theme: "CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOON."— Presentation transcript:
CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOON
Célia Regina Monte Barardi firstname.lastname@example.org Laboratório de Virologia Aplicada www.lvapli.ufsc.br Universidade Federal de Santa Catarina, Brasil
Peri Lagoon: Florianópolis, Santa Catarina, Brazil Surface area of 5.7 km² Water is distributed to the inhabitants (110,000 people) Recreational activities are allowed in the lagoon More than 2,000 visitors/spring and summer seasons
Gastroenteritis, diarrhea, and other diseases can be caused by viruses transmitted by fecal/urino-oral route HAdV – RVA – HAV - JCPyV Introduced into aquatic environments by human – industrial and agricultural activities Studies indicate these viruses as bioindicators of environmental contamination Introduction
Evaluate the presence of HAdV, JCPyV, HAV and RVA in the largest source of drinking water of the city of Florianópolis, Santa Catarina, Brazil (Peri Lagoon); Investigate their correlation to the waters physicochemical parameters and seasonal distribution of these viruses; Evaluate the integrity and infectivity of detected HAdV. Objectives Site 1: Center of the lagoon Site 2: Preserved environment Site 3: Degraded environment Site 4: Where water is collected for treatment and human consumption and also recreational activities
95.83% - HAdV (46/48) 64.5% - RV-A (31/48) 20.83% - JCPyV (10/48) 12.5% - HAV (6/48) 95.83% - HAdV (46/48) 64.5% - RV-A (31/48) 20.83% - JCPyV (10/48) 12.5% - HAV (6/48) Viral Distribution The seasonal distribution Results Site 1: Center of the lagoon Site 2: Preserved environment Site 3: Degraded environment Site 4: Where water is collected for treatment and human consumption and also recreational activities Winter: HAdV – HAV - RVA Summer and Spring: JCPyV
Correlation: Virus and physicochemical parameters Results
Integrity and viability of detected HAdV HAdV: Undamaged: 83.3% (10/12) Viability by PA: 66.6% (8/12) Viability by ICC-et-RT-qPCR 83.3% (10/12) Results
Surface water sources used for human consumption and recreational activities are being contaminated by human enteric viruses; The divergence among total genomic copy number, genomic copies of intact virus and the number of infectious viruses highlights the importance of including molecular and cell culture techniques during the environmental monitoring of viruses; ICC-et-RT-qPCR assay was sensible and, fast to detect viral particles infectious (viral mRNA) in water samples; Emphasizes the urgency of monitoring and implementation efficient methods for removing viral. Conclusions
Financial support: CNPq Project 470808/2009-8 CTAgro/CNPq/- 25/2010 CAPES: Process : AEX 11419/12-1 Team: Gislaine Fongaro, MSc student Mariana de Almeida do Nascimento, MSc student Aline Viancelli, PhD Denise Tonetta, MSc Maurício Mello Petrucio, PhD Célia Regina Monte Barardi, PhD
email@example.com Laboratório de Virologia Aplicada www.lvapli.ufsc.br Universidade Federal de Santa Catarina Gislaine Mariana N. Lucas ElMahdy Natália Dóris Camila Vanessa Mariana R.
Your consent to our cookies if you continue to use this website.