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CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOON.

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Presentation on theme: "CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOON."— Presentation transcript:

1 CORRELATION BETWEEN HUMAN VIRAL CONTAMINATION AND PHYSICOCHEMICAL PROFILES IN A SURFACE WATER LAGOON

2 Célia Regina Monte Barardi Laboratório de Virologia Aplicada Universidade Federal de Santa Catarina, Brasil

3 Peri Lagoon: Florianópolis, Santa Catarina, Brazil Surface area of 5.7 km² Water is distributed to the inhabitants (110,000 people) Recreational activities are allowed in the lagoon More than 2,000 visitors/spring and summer seasons

4 Gastroenteritis, diarrhea, and other diseases can be caused by viruses transmitted by fecal/urino-oral route HAdV – RVA – HAV - JCPyV Introduced into aquatic environments by human – industrial and agricultural activities Studies indicate these viruses as bioindicators of environmental contamination Introduction

5 Evaluate the presence of HAdV, JCPyV, HAV and RVA in the largest source of drinking water of the city of Florianópolis, Santa Catarina, Brazil (Peri Lagoon); Investigate their correlation to the waters physicochemical parameters and seasonal distribution of these viruses; Evaluate the integrity and infectivity of detected HAdV. Objectives Site 1: Center of the lagoon Site 2: Preserved environment Site 3: Degraded environment Site 4: Where water is collected for treatment and human consumption and also recreational activities

6 Enzymatic Assay (Integrity) Plaque Assay and ICC-et-RT-qPCR (Infectivity) Enzymatic Assay (Integrity) Plaque Assay and ICC-et-RT-qPCR (Infectivity) Methods HAdV Font: googleimagens

7 95.83% - HAdV (46/48) 64.5% - RV-A (31/48) 20.83% - JCPyV (10/48) 12.5% - HAV (6/48) 95.83% - HAdV (46/48) 64.5% - RV-A (31/48) 20.83% - JCPyV (10/48) 12.5% - HAV (6/48) Viral Distribution The seasonal distribution Results Site 1: Center of the lagoon Site 2: Preserved environment Site 3: Degraded environment Site 4: Where water is collected for treatment and human consumption and also recreational activities Winter: HAdV – HAV - RVA Summer and Spring: JCPyV

8 Correlation: Virus and physicochemical parameters Results

9 Integrity and viability of detected HAdV HAdV: Undamaged: 83.3% (10/12) Viability by PA: 66.6% (8/12) Viability by ICC-et-RT-qPCR 83.3% (10/12) Results

10 Surface water sources used for human consumption and recreational activities are being contaminated by human enteric viruses; The divergence among total genomic copy number, genomic copies of intact virus and the number of infectious viruses highlights the importance of including molecular and cell culture techniques during the environmental monitoring of viruses; ICC-et-RT-qPCR assay was sensible and, fast to detect viral particles infectious (viral mRNA) in water samples; Emphasizes the urgency of monitoring and implementation efficient methods for removing viral. Conclusions

11 Financial support: CNPq Project / CTAgro/CNPq/- 25/2010 CAPES: Process : AEX 11419/12-1 Team: Gislaine Fongaro, MSc student Mariana de Almeida do Nascimento, MSc student Aline Viancelli, PhD Denise Tonetta, MSc Maurício Mello Petrucio, PhD Célia Regina Monte Barardi, PhD

12 Laboratório de Virologia Aplicada Universidade Federal de Santa Catarina Gislaine Mariana N. Lucas ElMahdy Natália Dóris Camila Vanessa Mariana R.

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