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Published byDarin Burdell Modified about 1 year ago

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Lab session 2

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Use and care of the microscope Ocular lens (10X) Objective lens 4X, 10X, 40X and 100X ( oil immersion objective) Total magnification = magnification by the objective lens X magnification by the ocular lens (4) (10) = 40

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As the magnification increases microscopic field decreases 10X to locate object – see most of the slide Center the object in the field 40X Fine focusing to sharpen the image Drop of oil immersion on the slide

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Bring 100X place Fine focusing to sharpen image Make diagrams and write descriptions

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Bacteria exists in 3 basic shapes Cocci/coccus – spherical Bacilli/bacillus – rod shaped Spirillum – loose spiral Spirochete – tight spiral

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Bacteria exists in 3 major shapes chains Groups of two clusters

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Groups of two chain

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Budding yeast

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Borrelia burgdorferi

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