Presentation is loading. Please wait.

Presentation is loading. Please wait.

Spatial information: several germ layer specific Pou5f1 targets are transcriptional repressors: Pou5f1no Pou5f1 foxD3 Sox-independent her3 Sox-dependent.

Similar presentations


Presentation on theme: "Spatial information: several germ layer specific Pou5f1 targets are transcriptional repressors: Pou5f1no Pou5f1 foxD3 Sox-independent her3 Sox-dependent."— Presentation transcript:

1 Spatial information: several germ layer specific Pou5f1 targets are transcriptional repressors: Pou5f1no Pou5f1 foxD3 Sox-independent her3 Sox-dependent klf2b Sox-dependent Pou5f1no Pou5f1 Pou5f1 no pou5f1 Pou5f1 no pou5f1 Pou5f1no Pou5f1

2 promote differentiation (POD) Decision making in time time state reversible decision making spatial information input Pou5f1 promote decision repress decison open state - pluri- potent sharpen- ing decision fixed final states Sox act with Pou5f1 repress decison repress differentiation (ROD)

3 3 Temporal dynamics of Pou5f1 target network

4 Temporal behaviour of Sox, RODs and POD Simulation versus measurements with Pou5f1 without Pou5f1 “half” amount of Pou5f1 prediction “half” amount of Pou5f1 measurements differentiation genes prematurely activated in absence of Pou5f1 Pou5f1 targets: early “monophasic” activation (ROD) Pou5f1 + Sox targets: delayed “biphasic” activation (ROD)

5 Explanation for the Dual Role of Pou5f1 The dual role of Pou5f1 in activating promotors of differentiation (PoDs) and also repressing them indirectly by RODs allows to control the time of differentiation

6 Decision making in time time state reversible decision making spatial information input Pou5f1 promote decision repress decison open state - pluri- potent sharpen- ing decision fixed final states Sox acts with Pou5f1 repress PODs promote decision repress decisonrepress PODs FoxD3 ROD Type I Her3 ROD Type II

7 Decision making in time I: early Pou5f1 and late Pou5f1/Sox dependent activation time state reversible decision making spatial information input Pou5f1 promote decision open state - pluri- potent sharpen- ing decision fixed final states Sox acts with Pou5f1 promote decision

8 Decision making in time II: balance of repression and activation times developmental progress time state reversible decision making spatial information input Pou5f1 promote decision repress decison open state - pluri- potent sharpen- ing decision fixed final states Sox acts with Pou5f1 repress PODs promote decision repress decisonrepress PODs FoxD3 ROD Type I Her3 ROD Type II


Download ppt "Spatial information: several germ layer specific Pou5f1 targets are transcriptional repressors: Pou5f1no Pou5f1 foxD3 Sox-independent her3 Sox-dependent."

Similar presentations


Ads by Google