Presentation on theme: "Membrane Structure1 Chapter 11 Questions in this chapter you should be able to answer: Chapter 11- #s1 - 19."— Presentation transcript:
Membrane Structure1 Chapter 11 Questions in this chapter you should be able to answer: Chapter 11- #s1 - 19
Membrane Structure2 Membranes are described as a “2-dimensional liquid”. Why? Laser Tweezers Membrane Fluidity Jmol membrane model
Membrane Structure3 How can we measure membrane fluidity? FRAP: ‘Fluorescence Recovery After Photobleaching’ GFP: Green fluorescent protein Cell fusion also shows fluidity See Figure P 378 FRAP
Membrane Structure4 What factors determine how fluid a membrane is? Properties of P-lipids:Chain lengthSaturation Properties of membranes:Cholesterol contentCytoskeleton association
Membrane Structure5 What are the principal membrane lipids? Phospholipids Glycolipids Other membrane lipids (not phospholipids) Cholesterol Cerebrosides Sphingolipids Ceramide
Membrane Structure6 How are P-lipids distributed in lipid bilayers? P-lipids are synthesized on the ER membrane…. How do they get to other side of membrane? How is asymmetry achieved? Scramblase vs Flippase
Membrane Structure7 What are the principal functions of membrane proteins? How are membrane proteins connected to the membrane? Membrane associated (peripheral) Covalent vs Noncovalent Transmembrane (integral) Single-pass Multi-pass
Membrane Structure8 Why do transmembrane proteins occur as alpha helices and beta-pleated sheets?? Jmol Transmembrane proteins
Membrane Structure9 How can membrane proteins be purified and studied? -- detergents ‘mimic’ P-lipid structure around proteins Question 11-5 Why is red part hydrophilic and blue part hydrophobic?
Membrane Structure10 What do we know about the structure of bacteriorhodopsin? Function? Structure? Mechanism? Bacteriorhodopsin
Membrane Structure11 How is the cell membrane structurally reinforced? -- Cell cytoskeleton -- also influences fluidity
Membrane Structure12 Why are carbohydrates particularly abundant on the cell surface? Functions: Surface protectant Cell recognition Cell adhesion -- extracellular matrix
Membrane Structure13 How can protein movement in cell membrane be restricted? Consider challenge of intestinal epithelium… Fig p 395
Membrane Structure14 You have isolated two mutants of a normally pear-shaped microorganism that have lost their distinctive shape and are now round. One of the mutants has a defect in a protein you call A and the other has a defect in a protein you call B. You grind up mutant and normal cells separately and separate the plasma membranes from the cytoplasm by centrifugation. You then wash the membrane fraction with a low concentration of urea ( which disrupts their ability to interact with other proteins) and centrifuge the mixture. The membranes and their constituent proteins form a pellet while the proteins liberated by the urea wash remain in the supernatant. When you check each of the fractions for the presence of A or B, you obtain the results given below. First cell extract After urea wash and centrifugation MembraneCytosolMembraneSupernatant Normal cellsA and Bno A or BBA Mutant ABABno A or B Mutant BBABno A or B Answer the following statements about your results? (a) Which is an integral and which is a peripheral membrane-associated protein. The results for which cell-type shows this? (b) How does the mutation to protein-A alter its properties? (c) How does the mutation to protein-B alter its properties? (d) Which result(s) most indicate an interaction between A and B? Explain.