1. Epigenetic programming of monocyte-to-macrophage differentiation and trained innate immunity Dan Pensinger 2/17/15

2. Outline Background – Eukaryotic DNA Structure and Regulation – Epigenetics – Macrophage differentiation and signaling – Techniques used in the paper Results Summary

3. Eukaryotic DNA Structure and Regulation Genome, Smithsonian National Musuem of Natural History

4. Eukaryotic DNA Structure and Regulation Lawerence Berkeley National Laboratory

5. Epigenetic Histone Modifications The Alberta Epigenetics Network

6. Epigenetic Histone Modifications The Alberta Epigenetics Network

7. Epigenetic Histone Modifications The Alberta Epigenetics Network

8. Epigenetic Histone Modifications H3K27Ac promoters and enhancers Acetyl K27

9. Macrophage Differentiation and Signaling Siamon Gordon Nature Reviews Immunology 2003

10. Macrophage Differentiation and Signaling LPS Medvedev et al. J Endotoxin Res. 2006

11. Macrophage Differentiation and Signaling Invivogen

12. Cyclic AMP Signaling

13. Outline Background – Eukaryotic DNA Structure and Regulation – Epigenetics – Macrophage differentiation and signaling – Techniques used in the paper Results Summary

14. Paper Overview

15. Monocyte Isolation Miltenyi

16. Chromatin Immuno Precipitation (ChIP) Seq wikipedia

17. RNA-Seq Brian Haas and Micheal Zody, Nature Biotechnology 2010

18. DNase Seq Wang et al. PLoSOne 2012

19. Paper Overview

20. Figure 1

21. H3K27Ac promoters and enhancers Acetyl K27

22. Figure 1 P and E Enhancers Promoters

23. Figure 1

27. Figure 2

28. All MF MF MO LPS-MF BG-MF

29. Figure 2

30. All MF MO/LPS-MF BG-MF

31. Figure 2

32. BG- MF MO MF LPS- MF

33. Figure 2

34. Figure 3

36. BG- MF MO/LPS-MF All MF BG-MF MO All MF MF LPS-MF

37. Figure 4

38. ACe1 = BG-MF ACe2 = MO ACe3 = All MF ACe4 = MF ACe5 = LPS-MF

39. Figure 5

40. β-Glucan

41. Figure 5

42. Paper Overview