Presentation is loading. Please wait.

Presentation is loading. Please wait.

Gilt Acclimatization and Sow Health Project & Other Health Genomic Projects Bob Kemp PigGen Canada.

Similar presentations


Presentation on theme: "Gilt Acclimatization and Sow Health Project & Other Health Genomic Projects Bob Kemp PigGen Canada."— Presentation transcript:

1 Gilt Acclimatization and Sow Health Project & Other Health Genomic Projects Bob Kemp PigGen Canada

2

3 Why?  Need for the swine genetics industry to collaborate on genomics research  Need for the swine genetics industry to speak with one voice  Motivation to assist the competitiveness of the Canadian industry through developments in swine genomics  Clearly we were a stronger group working together

4 Current Members  Fast Genetics  Genesus  Génétiporc  Hypor Canada  La Coop fédérée  PIC Canada  Topigs Canada  Canadian Centre for Swine Improvement  Canadian Swine Breeders Association

5 Objectives  Represent the Canadian swine genetics industry with a single voice  Develop strategies and support for Canadian swine genetics research in concert with Canadian pork industry priorities

6 Research Priorities  Health and disease resistance  Meat quality and lean meat deposition  Sow lifetime productivity  Production efficiency  Animal welfare

7 Funding  Annual membership fees are paid by all active members  Support promotion, programs, small contribution research projects and operations  Large project funding  Project approved by 2/3 of the board  50% of individual members must contribute

8 Genomics of Health Projects  Canadian Projects  Gilt Acclimation  Genome Canada  PRRS/PHGC  Alberta Sire Line trial  Brandon Lillie  International Projects  USDA PRRS/PHGC  Other projects

9 Gilt Acclimation and Sow Health  Development of genetic selection tools to enhance sow health using a novel acclimation challenge model in Canadian commercial herds  Two Projects  Gilt acclimation and sow health phenotyping  Analyses and database development

10 Nucleus / AI Units Multiplier Herds Gilt Acclimation Commercial herds SAMPLES+DATASAMPLES+DATA Outbreak Herds Gilt Acclimation Project Overview

11 Data collection  Commercial herds identified by PigGen Canada members  Need to provide a health challenge  Target 30 herds  High health crossbred gilts  PigGen Canada member herds  Target 4,000 gilts  Groups of 20 to 50 gilts per entry, multiple entries per year

12 Data Collection  Gilt’s sire, dam, birth date and vaccination status  Blood samples prior to entry, 30 days after entry and at parities 1 and 2  Body weight collected at entry and day 30  Vaccination/treatment records, date of death and reasons during acclimation  Tissue/blood sample of dam of gilts

13 Data Collection  DNA extracted from gilt and dam blood samples  Gilts screened for exposure at entry, day 30, parities 1 and 2  PRRS, PCV2, SIV (H1N1 and H3N2), Actinobacillus pleuropneumoniae (APP), Mycoplasma hyopneumoniae (MH)  Standard sow breeding and production data thru parity 4  Sow health treatments, mortality and reasons

14 Outbreak 1  PRRS break Nov. 2011, blood drawn Dec. 2011  All sows in herd confirmed PRRS exposure  Exact date of exposure unknown

15 Outbreak 2  Herd confirmed PRRS exposure  50 PRRS negative gilts exposed to herd PRRS virus on entry to quarantine  Gilts bled at 2 week intervals (0, 2,4,6,8)  Elisa (IgG), SN antibody titres and qPCR in serum over the five time points

16 Nucleus / AI Units Multiplier Herds Gilt Acclimation Commercial herds SAMPLES+DATASAMPLES+DATA Outcomes General immune capacity of crossbred gilts GWAS of general immune response of sows GBVs using sow health data Integrated database resource and collection system Outbreak Herds Gilt Acclimation Project Overview

17 Gilt Acclimation Project Status  25 Herds in Alberta, Saskatchewan, Manitoba, Ontario and Quebec  Over 2,000 gilts sampled  Day 0 and 30 samples sent to Delta Genomics laboratory  Data collection using standardized forms  Serum analysis begun  Database development started

18 Gilt Acclimation Project Status  Outbreak 1  60K genotype on 600 affected sows  Litter data on most sows post-PRRS exposure  2 years of historical litter data pre-PRRS infection  Outbreak 2  Examine variation in response  60K genotype strategy being developed  Herd/gilt reproductive and litter data being collated

19 Nucleus / AI Units Multiplier Herds Gilt Acclimation Commercial herds SAMPLES+DATASAMPLES+DATA Outcomes Genetic selection tools Early life indicators of health and performance Reduced cost of production Improved sow health, longevity and welfare Health data genetic collection system and data resource Leverage industry funding Reinforce Canada’s high health pork and genetic reputation Benefits General immune capacity of crossbred gilts GWAS of general immune response of sows GBVs using sow health data Integrated database resource and collection system Outbreak Herds Gilt Acclimation Project Overview

20 Application of genomics to improve swine health and welfare  Genome Canada and many Canadian and international groups  Overall goal Provide new genomic-based, diagnostic tools to select pigs that are genetically less susceptible to PRRS and PCVAD  Builds on existing projects both within Canada and internationally  Very large leverage of existing funding

21 Application of genomics to improve swine health and welfare  Activities  Genomics and it’s ethical, economic, environmental, legal and social aspects  Focus on developing risk-perception models to characterize Canadian attitudes and perceptions about the use of genomic technologies in pork production  Phenomic resources for genomics of swine health  Combine challenge experiments, industry datasets and in vitro analyses

22 Application of genomics to improve swine health and welfare  Activities  Next-generation genomic analysis of swine health (genome function focus)  Include genome wide association studies, trancriptomics, proteomics and kinomics  Application strategies to deliver healthier pigs  Reduced SNP panels for selection, genomic estimated breeding values, integration into existing improvement programs

23 PRRS Consortium (PHGC) - Kansas PCV2 Consortium Nebraska Gilt Acclimation PigGen Canada Pregnant Gilt Challenge Saskatchewan SAMPLES+DATASAMPLES+DATA Project database 60k SNP chip Transcriptomics: Gene expression Proteomics: proteins and pathways Genome Wide Associations Genomic Breeding Values (GBVs) Models of PRRS infection dynamics Application Kinomics: protein kinases In Vitro Assays

24 Canadian Component of the PRRS Host Genetics Consortium  Groups involved  Alberta Livestock Genomics Program  Livestock Gentec / U of Alberta  PigGen Canada  USDA – ARS  Goals  Complete 60K genotyping on final 3 groups  GWAS completed on all 1600 pigs  Enhance identification of genes and pathways (transcriptomics)  Enhance identification of biomarkers (proteomics)  Transfer technology to Canadian lab

25 Establishing a commercial dataset to support genomics of swine health  Builds on an existing sire line project with Sunhaven Farms (AB) and Gowans Feed Consulting  Opportunity to  collect detailed performance and health data on approximately 8,000 pigs  identify genetic variation for performance and susceptibility to specific diseases  large scale validation of the utility of DNA panels for better immune response

26 Establishing a commercial dataset to support genomics of swine health  Deliverables  Collect health/mortality data and tissue sample on 8,000 pigs, supplemented by detailed performance data on 2,100 of the pigs  Determine variation in terminal sire lines with respect to performance and susceptibility of disease within this system  Recommendations on procedures required for future health data collection from commercial systems  Effect of DNA marker panels developed for improved immune response or for susceptibility to PRRS and PCVAD

27 Dr. Brandon Lillie, University of Guelph  Research program in genomics of health  Immune response panel development  Found several markers (SNP) associated with pigs diagnosed at necropsy with various diseases or pathogens  Results suggest that these SNPs are associated with disease susceptibility  Might be genetic markers of impaired innate immune function  Immune response panel will be evaluated in other projects and data sets

28 International Projects  PRRS Host Genetics Consortium (PHGC)  USDA lead project with many participants  Pigs sourced from breeding companies  Weaned pig challenge model for PRRS  Weights, viral load and many other samples and blood data  8 tests, 1600 pigs  Salmonella resistance (USA, UK, Netherlands)  Immune response (Germany, UK, France)

29 International Projects  Mycoplasmal pneumonia (Japan)  Psuedorabies virus (Germany)  Protein biomarkers for infectious disease (Netherlands)

30 Thoughts on the use of genomics for health  Use of genomic information will become integrated into selection programs  Data collection systems will be key for research, improvement and monitoring of health outcomes  Large, integrated databases will be required for development and validation  Prediction of individual pig susceptibility to disease within a given environment (Biomarkers)

31 Thank you for your attention!


Download ppt "Gilt Acclimatization and Sow Health Project & Other Health Genomic Projects Bob Kemp PigGen Canada."

Similar presentations


Ads by Google