1. Study on HEV Vaccination Approach in Swine for Public Health protection Jeanette van der Goot, Jantien Backer, Leo van Leengoed, David Rodriquez Lazaro, Franco Ruggeri, Alessandra Berto, Francesca Martelli, Ivo Pavlik, Wim H. M. van der Poel

2. 2 Pig finishing farm industry finishing farm Weaning 10 weeks of age ± 25 kg Slaughter 28 weeks of age ± 110 kg

3. 3 SEIR Model for HEV in pigs susceptible Experimental data (Bouwknegt et al. 2007): Transmission parameter: 0.66 infections per day per infectious animal Latent period: 3 (0.8 – 6.3) days Infectious period: 25.8 (7.0 – 54) days latently infected infectiousrecovered

4. 4 Age distribution at high transmission weeks of age age at entryage at slaughter proportion of population R 0 = 17 susceptible latently infected infectious recovered total

5. 5 Age distribution at lower transmission weeks of age age at entryage at slaughter proportion of population R 0 = 2.6 susceptible latently infected infectious recovered total

6. 6 Infection dynamics at high transmission time (days) time of infection number of animals susceptible latently infected infectious recovered total R 0 = 17

7. 7 Infection dynamics at lower transmission time (days) time of infection number of animals susceptible latently infected infectious recovered total R 0 = 2.6

8. 8 Infectious period distribution infectious period (days) uncensored censored

9. 9 Partners/ sampling countries United KingdomVLA (data available) Czech RepublicVRI SpainITACyL ItalyISS NetherlandsUtrecht University

10. 10 Farm sampling schedule One HEV-positive farm per country Sampling by taking anal swabs Sampling of 2 pigs per pen Total nr of samples in a farm of 10 compartments incl. 10 pens: 2 * 10 * 10 = 200 samples

11. 11 Time schedule task 6.4 Farm sampling:April 2009 – June 2010 Testing of samples:June 2009 – August 2010 Data evaluations: August 2010 – Dec 2010 Modeling:Jan 2011- Feb 2011 Publication:March 2011- April 2011