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Figure S1 a. Neutrophilsb. Non-inflammatory monocytes c. Macrophages or DCs d. Inflammatory monocytes SSC FSC B C SSC * * * * * III Count F4/80 A.

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Presentation on theme: "Figure S1 a. Neutrophilsb. Non-inflammatory monocytes c. Macrophages or DCs d. Inflammatory monocytes SSC FSC B C SSC * * * * * III Count F4/80 A."— Presentation transcript:

1 Figure S1 a. Neutrophilsb. Non-inflammatory monocytes c. Macrophages or DCs d. Inflammatory monocytes SSC FSC B C SSC * * * * * III Count F4/80 A

2 A B Densitometry unit pNF-ĸB p65 pSTAT3 Figure S2 IKKβ ΔMye C57BL/6 Densitometry unit pSTAT3 pNF-ĸB p65

3 PBS (3 hours) EdTx (6 hours) C57BL/6 IKKβ ΔMye Treatment (time) EdTx (3 hours) C57BL/6 IKKβ ΔMye C57BL/6 IKKβ ΔMye Figure S3

4 CCL2 CXCL2 * * * * * * * * * * Relative mRNA levels (20 ― ∆Ct) Figure S4 Relative mRNA levels (20 ― ∆Ct) B A

5 * Ab titers (Log 2 GMTs) PA-specific neutralizing Abs Ab titers (Log 2 GMTs) PA-specific serum IgG titers A * B Figure S5

6 F1V-specific Ab titers (Log 2 GMTs) C57BL/6 IKKβ ΔMye Figure S6 Serum IgA F1V-specific Ab titers (Log 2 GMTs) Fecal IgA A B C57BL/6 IKKβ ΔMye

7 Figure S7 CD11b + F4/80 – Gr-1 high (Neutrophils) Frequency (%) in myeloid cells Days after injection A B CD11b + F4/80 – Gr-1 high (Neutrophils) Frequency (%) in myeloid cells CD11b + F4/80 + Gr-1 high (Inflammatory monocytes) CD11b + F4/80 + Gr-1 low (Macrophages or DCs) Frequency (%) in myeloid cells CD11b + F4/80 + Gr-1 ─ (Non-inflammatory monocytes)

8 Figure S8 Frequency (%) B220 + IL-17RA low B220 + IL-17RA high B ▼ * * ▼ ▼ * ▼ * C57BL/6 CD11b + IKKβ ΔMye CD11b + EdTx + – – – + – – – + + – – – + – – – + FSC IL-17RA A IL-17RA low IL-17RA high APRIL BAFF AID * ▼ ▼ ▼ ▼ * ▼ ▼ Relative mRNA levels (20 ― ∆Ct) C57BL/6 CD11b + IKKβ ΔMye CD11b + EdTx + – – – + – – – + C

9 Pro-inflammatory cytokine mRNAs (20 ― ∆Ct) Anti-inflammatory Cytokine mRNAs (20 ― ∆Ct) IL-1βIL-6 C57BL/6 CD11b + IKK  Δ Mye CD11b + ++–– +–+– ––++ EdTx ++–– +–+– ––++ * ▼ ▼ ▼ ▼ Enzyme mRNAs (20 ― ∆Ct) Caspase-1 * ▼ IL-10 TGF-  * * ▼ ▼ Figure S9 TNF-αIL-23 ▼ ▼ * * ▼ * C57BL/6 CD11b + IKK  Δ Mye CD11b + ++–– +–+– ––++ EdTx ++–– +–+– ––++ C57BL/6 CD11b + IKK  Δ Mye CD11b + ++–– +–+– ––++ EdTx A B C


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