Presentation is loading. Please wait.

Presentation is loading. Please wait.

Myeloproliferative Disorders / Neoplasms Intro for the Internist Satish Shanbhag MBBS, MPH Assistant Professor of Medicine and Oncology Johns Hopkins University.

Similar presentations

Presentation on theme: "Myeloproliferative Disorders / Neoplasms Intro for the Internist Satish Shanbhag MBBS, MPH Assistant Professor of Medicine and Oncology Johns Hopkins University."— Presentation transcript:

1 Myeloproliferative Disorders / Neoplasms Intro for the Internist Satish Shanbhag MBBS, MPH Assistant Professor of Medicine and Oncology Johns Hopkins University School of Medicine

2 Objectives Internist focused review of the presentation, diagnostic workup, treatment and prognosis of the common myeloproliferative neoplasms

3 Disclosures None

4 Stem Cell Basis of Hematopoiesis NormalMyeloproliferative disease Stem cells Progenitors Blood

5 The Chronic Myeloproliferative Disorders Acquired hematopoietic stem cell disorders characterized by overproduction of one or more of the formed (mature) elements of the blood Complicated by bone marrow expansion, extramedullary hematopoiesis (liver, spleen, l.n), myelofibrosis and transformation to acute leukemia Transition between phenotypes common with latency period of decades

6 The Big 4 Chronic myeloid leukemia Polycythemia vera Essential thrombocythemia Primary myelofibrosis Chronic Eosinophilic leukemia Systemic Mastocytosis

7 Chronic Myeloid Leukemia Unregulated proliferation of myeloid cells in the bone marrow and accumulation of these cells in the blood Increased mature granulocytes (neutrophils, eosinophils and basophils) and their precursors in the blood Characteristic chromosomal translocation called the Philadelphia chromosome Bcr-abl translocation detectable in the blood by karyotyping, FISH or PCR.

8 Melo. Blood. 1996;88:2375. Pasternak et al. J Cancer Res Clin Oncol. 1998;124:643. Ph Chromosome → bcr-abl gene P 210 FUSION PROTEIN WITH CONSTITUTIVE TYROSINE KINASE ACTIVITY bcr-abl bcr Philadelphia Chromosome (or 22q-) Chromosome 9 q+ abl Chromosome 9 Chromosome 22

9 Typical presentation of chronic phase CML

10 Faderl et al. Ann Intern Med. 1999;131:207. Goldman. Curr Opin Hematol. 1997;4:277. CML: Clinical Presentation Chronic Phase ~ 85% Common symptoms: – fatigue – weight loss/anorexia – Early satiety – asymptomatic in ~ 50% Common signs: – palpable splenomegaly – basophilia Accelerated Phase and Blast crisis More aggressive disease with increased blasts and poorer prognosis

11 CML: Pre-Imatinib Survival Years from referral 432108765 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 Cumulative proportion surviving CML PhaseTotalDead Chronic24491043 Accelerated479276 Blastic285219 5 mo28 mo71 mo

12 Imatinib Mesylate – Targeted therapy in cancer BCR-Abl tyrosine kinase enzyme exists only in clonal cancer cells and not in normal patient cells Imatinib is a Tyrosine-kinase inhibitor which prevents the BCR-Abl enzyme product from initiating the signalling cascade necessary for cancer development, thereby causing cancer cell apoptosis More ‘POTENT’ TKIs Nilotinib, Bosutinib, Dasatinib, Ponatinib have subsequently been developed. Bone marrow / stem cell transplant for select patients

13 Survival in newly diagnosed CP-CML by year of therapy. Kantarjian H et al. Blood 2012;119:1981-1987 ©2012 by American Society of Hematology


15 JAK2 V617F in Myeloid Disease One mutation, many phenotypes DiseaseJAK2 V617F Prevalence PV97% ET60% PMF60%

16 Janus kinase 2 (JAK2), is a gene on the short arm of chromosome 9 that encodes for a cytoplasmic tyrosine kinase A mutation in the JAK2 gene leads to constitutive tyrosine phosphorylation activity that promotes hypersensitivity to cytokines / growth factors and induces epo-independant erythrocytosis.

17 JAK2 G5073770T JAK2 V617F Scott, et al Lancet 2005 Signal transduction lesions in the MPN

18 JAK2 V617F : phenotypes of disease ET PV PMF Transition between phenotypes common with latency period of decades JAK2V617F

19 Bone Marrow Biopsy Normocellular bone marrow

20 Bone Marrow Biopsy Hypercellularity and clustering of atypical megakaryocytes

21 Polycythemia Vera

22 Why is he erythrocytotic?

23 Polycythemia Vera Elevated totally body red cell mass; Hgb, Hct are surrogate markers Rule out dehydration, epo driven erythrocytosis Chronic disease with median survival of > 15-20 years with current therapy

24 Diagnostic Criteria for PV (PVSG) Major criteriaMinor criteria 1.Increased red cell mass1. Platelet count >400,000/microL Males: ≥36 mL/kg 2. White blood cell count >12,000/microL* Females: ≥32 mL/kg3. Serum vitamin B12 >900 pg/mL 2. Arterial oxygen saturation ≥92 % 3. Splenomegaly Low serum epo levels JAK 2 V617F mutation Pancytosis Bone Marrow panmyelosis

25 Objectives of therapy 1. Symptom control – prevent pruritus, headaches 2. Prevent thromboses 1. Phlebotomy (goal Hct <45% men or <42% female ) 2. Aspirin 81 mg po qd 3. Cytoreductive agents such as hydroxyurea

26 Essential Thrombocythemia

27 ET  secondary myelofibrosis

28 Essential Thrombocythemia Chronic non-reactive thrombocythemia that does not fit criteria for other MPNs – Headache – Syncope – Atypical chest pain – Livedo reticularis – Erythromelalgia (burning pain of the hands or feet associated with erythema and warmth) 60% patients positive for JAK2 V617F

29 Treatment Young low risk patients may not need any therapy or just a baby aspirin daily Higher risk patients (those with prior h/o clots or elderly) benefit from cytoreduction with Anagrelide or Hydroxyurea n engl j med 353;1 july 7, 2005

30 Thrombocytosis and Acquired VW syndrome

31 Primary Myelofibrosis Megakaryocyte proliferation and atypia, usually accompanied by reticulin and/or collagen deposition in the marrow Demonstration of a clonal marker (eg, JAK2 or MPL) Leukoerythroblastosis on smear does not met criteria for other MPN Splenomegaly Anemia

32 Bone marrow in PMF Modern Pathology (2012) 25, 1193–1202

33 Blood smear in PMF

34 PMF / secondary myelofibrosis PMF carries the worst prognosis among the MPNs, although survival is still measured in years PV and ET can show gradual progression to secondary myelofibrosis over decades

35 Therapies for PMF Bone marrow transplant is the only cure Ruxolitinib (JAK inhibitor) is a recently approved drug for treating symptomatic PMF that also reduces splenomegaly Hydroxyurea, Thalidomide and its derivatives have also been studied but not FDA approved

36 Hopkins500: Natural History Diagnosis 10 years 20 years N=405N=283N=57 slide courtesy Dr.Alison Moliterno

37 Thanks Dr. Doug Smith and Dr.Alison Moliterno for sharing slides and images

Download ppt "Myeloproliferative Disorders / Neoplasms Intro for the Internist Satish Shanbhag MBBS, MPH Assistant Professor of Medicine and Oncology Johns Hopkins University."

Similar presentations

Ads by Google