Presentation on theme: "Weaning mice. Remove weaning-age mice (usually 21 days) from the parent cage. Separate the pups by sex. Mark the parent cage with a separation card; mark."— Presentation transcript:
Remove weaning-age mice (usually 21 days) from the parent cage. Separate the pups by sex. Mark the parent cage with a separation card; mark the weaned cages with separation stickers
Before you start Check the problem log to see if you expect to have any separations Check the room for green OC cards
Before you start Check your watch!! What time is it? If you don’t have a watch, check the time on your pager
Why are you checking the time? So you can fill out the Special Services Log when you are done. DatePrincipal Investigator’s Name Types of Service Cage Separation (# of cages) Types of Service Other Services Number of Hours Performed By (Print Name) Initials
Before you start Double-check the OC card –Is it a supervisor check? –Double-check that the weaning due date is correct- look at the weaning date calendar.
Weaning mice Remove weaning-age mice (usually 21 days) from the parent cage. Separate the pups by sex. Complete the necessary paperwork
Sex pups 2 at a time If they look the same, try again until you get one that looks different. The one with the longer anogenital distance is the male. The other is the female. Keep one pup of known sex, and use it to compare against the rest of the pups.
Housing pups 5 females per cage 4 males per cage Recheck the sex Put pellets of food on the cage floor Complete the green separation cards and stickers House the separated cages on the separation shelf
Separation cards The white sticker (with the separation number) goes on the green card. Write the date and the number of new cages created on the white sticker
Fill out the Special Services Log. Fill out the Log. Put the number of new cages created in the separation column. Look at your watch to determine the time to complete the separation. The whole time (including paperwork) counts.