Content 1. PRINCIPLES Principles of Lentikats Biotechnology Benefits of Lentikats Biotechnology Lentikats Biotechnology in distilleries 2. CASE STUDIES Case study – molasses distillery Case study – distillery processing sugar solutions Case study – corn distillery 3. WHAT CAN WE OFFER? References and experiences, our partners Contacts
What is a Lentikats Biocatalyst? Unique shape of the matrix and the original principle of immobilisation enable the encapsulation of free enzymes or microorganisms (bacteria, yeasts, fungi) in a stable porous PVA (polyvinylalcohol) carrier. The carrier‘s advantages are: Excellent physio-mechanical characteristics that provide for long-term mechanical stability Low biodegradability Zero toxicity Lentikats Biocatalyst enables easy separation of enzymes and microorganisms from the surrounding media and therefore it can be reused multiple times. Biotechnologies are changing the world…
Lentikats Biocatalyst Advantages of using PVA as a matrix for encapsulation: inexpensive, non-toxic polymerization under optimal conditions, cell conservation enables creation of hydrogels with excellent mechanical properties no side effects in processes large capacity for cell building easily supervised processes stable processes easy separation from bulk media
How does Lentikats Biocatalyst function? Due to its maximum surface and at the same time minimum volume of the Lentikats Biocatalyst, the diffusion limitations associated with the transport of the substrate into the lens and the product out of the lens are significantly reduced.
What are the advantages of Lentikats Biotechnology over traditional technologies? Higher biomass concentration Reaction rate Biomass concentration Reduced volume of reactors Investment costs saving Operational costs saving (electricity, heat, etc.) Higher yields 1) 100 € 11
What are the advantages of Lentikats Biotechnology over traditional technologies? Easy separation from bulk media Repeatable use of Biocatalyst Operational costs saving Lower production of waste and by-products 100 € Applicability under extreme or stress conditions 2) 3) Robustness towards environmental shocks profound change of pH level higher concentration of toxic compounds
Technological process set-up Legend: 1.Sugars media inlet 2.Fermented media 3.Inlet for Lentikats Biocatalyst filling 4.Outlet for Lentikats Biocatalyst emptying 5.Sieve separator (angle slop 15-20°, aperture 0.8-1 mm) 6.Liquid fermentation broth with Lentikats Biocatalyst 7.Liquid fermentation broth without Lentikats Biocatalyst 8.Gear with speed change box 9.Stirrer 10.pH probe well 11.Temperature probe well 12.Antifoam agent inlet 13.Liquid level measurement (stainless steel pressure probe)
Which substrates can be used for Lentikats Biotechnology? Lentikats Biocatalysts provide a solution for the production of bioethanol from the following substrates: 1.Molasses, beet juice and sugar cane 2.Starch-containing (cereal) materials (corn, wheat, etc.) 3.Ligno-cellulose containing materials (straw, pollard…)
Our products Lentikats Biocatalyst for the fermentation of sucrose, glucose and lactose solutions (immobilized bacteria Zymomonas mobilis, yeast Saccharomyces cerevisiae, Kluyveromyces sp.) Lentikats Biocatalyst for the 2nd step of hydrolyses of liquefied starch solution (immobilized enzyme glukoamylase) Lentikats Biocatalyst for the production of inverted sugar from molasses (immobilized enzyme invertase) Energy is all around us… …the key is to obtain it effectively… …and protect the environment at the same time…
Biotechnologies are changing the world….. Economic comparison of traditional technology and Lentikats Biotechnology for molasses distillery
Biotechnologies are changing the world….. Reactor volume reduction – reduced investments Traditional technologyLentikats Biotechnology Fermentation volume3,450 m 3 360 (2 x 180) m 3 Reserve reactor - 180 m 3 Pre-fermentation volume213 m 3 - Volume for preparation of nutrients16 m 3 - Equalisation tank before distillation - 180 m 3 Sanitation tank48 m 3 Total 3,727 m 3 768 m 3 Production of ethanol39,035,667 L/year41,666,667 L/year Process yield290 L/tons309 L/tons
Biotechnologies are changing the world….. Lentikats Biocatalyst consumption (125,000 L a.a. per day) * Guaranteed lifetime ** Assumed lifetime Biocatalyst typeLifetime Annual consumption of Lentikats Biocatalyst Price (€/year) immobilised yeast Saccharomyces cerevisiae 8*–12* months 61.5*–41** tons 790,000 –526,500 Biotechnologies that are environmentally friendly …
Biotechnologies are changing the world….. Profit of ethanol production at the capacity of 125,000 L a.a. per day Traditional technologyLentikats Biotechnology Ethanol production (m 3 /year a.a.) 39,03641,667 Ethanol yield from molasses (L/ton) 290309 Annual revenues for ethanol (€) 24,600,00026,500,000 Gross profit without depreciation (€) 7,250,0008,185,000 Solution for higher efficiency and stability…
Case study – distillery processing sugar solutions (sugar cane or sugar beet juice)
Sugar distillery (125,000 L a.a. per day, continuous mode) sugar solution 45 tons of Lentikats Biocatalyst 140 g/L TFS * * Total Fermentable Sugars 41 667 m 3 /year a.a.
Biotechnologies are changing the world….. Biotechnologies are changing the world….. Fermentation conditions Zymomonas mobilis continuous mode 180g saccharose. hour -1. kg -1 temperature: 30–35°C, pH 4.8–5 ethanol yield 95 % 9.02 vol. % ethanol
Economic comparison of traditional technology and Lentikats Biotechnology for distillery processing sugar solutions
Biotechnologies are changing the world….. Biotechnologies are changing the world….. * Guaranteed lifetime, ** assumed lifetime Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biocatalyst typeLifetime Annual consumption of Lentikats Biocatalyst * Price (€/year) immobilised bacteria Zymomonas mobilis 1.5*–2** years 27.4*–20.5** tons 351,500 –263,500 Biotechnologies that are environmentally friendly …
Profit of ethanol production at the capacity of 125,000 L a.a. per day Traditional technologyLentikats Biotechnology Ethanol production (m 3 /year a.a.) 39,03641,667 Annual revenues for ethanol (€) 24,600,00026,500,000 Gross profit without depreciation (€) 7,240,0008,630,000 Solution for higher efficiency and stability…
Biotechnologies are changing the world….. Biotechnologies are changing the world….. Fermentation conditions Zymomonas mobilis continuous mode 180g glucose. hour -1. kg -1 temperature: 30–35°C, pH 4.8–5 ethanol yield 95 % 9.21 vol. % ethanol
Economic comparison of traditional technology and Lentikats Biotechnology for corn distillery
Reactor volume reduction – reduced investments Traditional technologyLentikats Biotechnology Mixing tank14 m 3 Fermentation reactor26 m 3 Saccharification reactor9 m 3 36 m 3 Fermentation volume552 m 3 72 (2 x 36) m 3 Reserve reactor-36 m 3 Pre-fermentation volume86 m 3 - Sanitation tank9 m 3 Equalisation tank before distillation-26 m 3 Total696 m 3 219 m 3 Production of ethanol4,473,667 L/year5,000,000 L/year Process yield344 L/tons385 L/tons
Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biocatalyst typeLifetime Annual consumption of Lentikats Biocatalyst * Price (€/year) immobilised enzyme glucoamylase 1,200*–1,500** hours 11.9*–9.5** tons 150,000 –120,000 immobilised yeast Saccharomyces cerevisiae 1.5*–2** years 3.6*–2.7** tons 46,000 –34,500 Biotechnologies that are environmentally friendly … * guaranteed lifetime, ** assumed lifetime
Profit of ethanol production at the capacity of 15,000 L a.a. per day Traditional technologyLentikats Biotechnology Ethanol production (m 3 /year a.a.) 4,4745,000 Ethanol yield (L/ton of corn) 344385 Annual revenues for ethanol (€) 2,817,0003,150,000 Gross profit without depreciation (€) 1,370,0001,600,000 Solution for higher efficiency and stability…
Other Lentikats Biotechnology Applications Lentikats Biocatalysts and applied Lentikats Biotechnology also deliver additional value and benefits in the following sectors : waste water treatment – nitrogen removal pharmaceutics – production of robust biocatalysts food industry – whey processing, production of special sugars carbohydrates and syrups Biotechnologies are changing the world…
Lentikats Biotechnology services Monitoring of specified technological procedures and conditions. Periodical monitoring of Lentikats Biocatalyst’s activity. Supply and replacement of Lentikats Biocatalyst in such periods and volumes to sustain the required Lentikats Biotechnology parameters. Disposal of the used Biocatalyst.
Have we raised your interest in testing Lentikats Biotechnology in your own project? We are prepared to supply a sufficient amount of our Biocatalyst and provide full assistance in order to convince you of the obvious advantages our technology has when applied in your distillery. For all companies introducing new technologies, the first applications and end results are the most important ones. That is why we offer excellent conditions to potential clients in an effort to eliminate any of their risks. What can we offer to you?
Guarantee of your investments and the investment return You can test our biocatalyst easily by yourself with your substrate in your own plant or plant of your choice to see the results. We offer to you our assistance and full support at: - pilot model installation - installation into an existing reactor Load of Biocatalyst for testing is free of charge.
Our know-how, references and experiences 1.Production of bioethanol from sugar beet molasses in Bioferm Kolín distillery with a capacity of 6 litres of absolute alcohol per hour. 2.Processing of technical-technological documentation for the supply to an agricultural refinery with a production capacity of cca 3 000 m 3 of bioethanol per year, based on our concept of Bio Plant 2015. 3.Processing of technical documentation for the delivery of a distillery with a production capacity of cca 450 hl of bioethanol per day utilising corn as a raw material. 4.Production of bioethanol from starch milk of type B in Krnov distillery with a capacity of 12 litres of absolute alcohol per hour. 5.Process optimisation and processing of technical documentation for a reconstruction of the existing distillery in India which uses cane molasses as a substrate.
Our partners in the field of industrial applications Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India www.fermentabiotech.com VÚCHZ, a.s., Křižíkova 70, 612 00 Brno, CZ www.vuchz.cz Gresham’s Eastern (Pvt) Ltd. F-302 Sind Industial Trading Estate Karachi http://www.gel1947.com Zinchem, Námestie Slobody 5, 071 01 Michalovce, SK www.zinchem. sk geniaLab®, BioTechnologie-Produkte und Dienstleistungen Gmbh Büchnerstraße 7, 38118 Braunschweig, Germany www.genialab.com
Our partners in the field of research and development ICT Prague, Department of Fermentation Chemistry and bioengineering, Technická 5, 166 28 Praha 6, CZ www.vscht.cz Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India www.fermentabiotech.c om CHEMCOMEX Praha, a.s., Pražská 810/16, 102 21 Praha 10, CZ www.chemcomex.cz Institute of Microbiology, Academy of Science of the Czech Republic Vídeňská 1083, 142 20 Praha 4, Krč, CZ www.biomed.cas.cz STU Bratislava, Faculty of chemical and food technology, Institute of biotechnology and food technology, Radlinského 9, 81237 Bratislava, SK www.stuba.sk Association of Alcohol Producers in Slovakia, Záhradnícka 21, 811 07 Bratislava, SK www.azzz.sk/clenovia.ph p?zvez=zvll Sekab, Box 286, s-891 26 Örnsköldsvik, Sweden www.sekab.com
Thank you for attention Company Headquarters: LentiKat´s a.s. Evropska 423/178 160 00 Prague 6 Czech republic Tel.:+ 420 224 362 461 Fax:+ 420 224 362 463 E-mail:email@example.com Production plant and laboratories LentiKat´s a.s. Pod Vinicí 83 471 27 Stráž pod Ralskem Czech republic Tel.:+ 420 487 851 393 Fax:+ 420 487 851 399 E-mail: firstname.lastname@example.org