2Content 1. PRINCIPLES Principles of Lentikats Biotechnology Benefits of Lentikats BiotechnologyLentikats Biotechnology in distilleries2. CASE STUDIESCase study – molasses distilleryCase study – distillery processing sugar solutionsCase study – corn distillery3. WHAT CAN WE OFFER?References and experiences, our partnersContacts
4What is a Lentikats Biocatalyst? Unique shape of the matrix and the original principle of immobilisation enable the encapsulation of free enzymes or microorganisms (bacteria, yeasts, fungi) in a stable porous PVA (polyvinylalcohol) carrier. The carrier‘s advantages are:Excellent physio-mechanical characteristics that provide forlong-term mechanical stabilityLow biodegradabilityZero toxicityLentikats Biocatalyst enables easy separation of enzymes and microorganisms from the surrounding media and therefore it can be reused multiple times.Biotechnologies are changingthe world…4
5Lentikats Biocatalyst Advantages of using PVA as a matrix for encapsulation:inexpensive, non-toxicpolymerization under optimal conditions, cell conservationenables creation of hydrogels with excellent mechanical propertiesno side effects in processeslarge capacity for cell buildingeasily supervised processesstable processeseasy separation from bulk media
6Lentikats Biocatalyst Production Lentikats Biocatalyst Application Lentikats Lentikats Biocatalyst & Biotechnology (our product) (application process)Lentikats Biocatalyst Production Lentikats Biocatalyst Application
7How does Lentikats Biocatalyst function? Due to its maximum surface and at the same time minimum volume of the Lentikats Biocatalyst, the diffusion limitations associated with the transport of the substrate into the lens and the product out of the lens are significantly reduced.7
11What are the advantages of Lentikats Biotechnology over traditional technologies? 1)Higher biomass concentrationReduced volume of reactorsReaction rateInvestment costs savingBiomass concentration100 €100 €100 €100 €Operational costs saving (electricity, heat, etc.)Higher yields
12What are the advantages of Lentikats Biotechnology over traditional technologies? 100 €100 €2)Easy separation from bulk media100 €Operational costs saving100 €Repeatable use of BiocatalystLower production of waste and by-products3)Robustness towards environmental shocksprofound change of pH levelhigher concentration of toxic compoundsApplicability under extreme or stress conditions
14Technological process set-up Legend:Sugars media inletFermented mediaInlet for Lentikats Biocatalyst fillingOutlet for Lentikats Biocatalyst emptyingSieve separator (angle slop 15-20°, aperture mm)Liquid fermentation broth with Lentikats BiocatalystLiquid fermentation broth without Lentikats BiocatalystGear with speed change boxStirrerpH probe wellTemperature probe wellAntifoam agent inletLiquid level measurement (stainless steel pressure probe)
15Which substrates can be used for Lentikats Biotechnology? Lentikats Biocatalysts provide a solution for the production of bioethanol from the following substrates:Molasses, beet juice and sugar caneStarch-containing (cereal) materials (corn, wheat, etc.)Ligno-cellulose containing materials (straw, pollard…)
16Our productsLentikats Biocatalyst for the fermentation of sucrose, glucose and lactose solutions (immobilized bacteria Zymomonas mobilis, yeast Saccharomyces cerevisiae, Kluyveromyces sp.)Lentikats Biocatalyst for the 2nd step of hydrolyses of liquefied starch solution (immobilized enzyme glukoamylase)Lentikats Biocatalyst for the production of inverted sugar from molasses (immobilized enzyme invertase)Energy is all around us… …the key is to obtain it effectively… …and protect the environment at the same time…
21Economic comparison of traditional technology and Lentikats Biotechnology for molasses distillery Biotechnologies are changing theworld…..
22Reactor volume reduction – reduced investments Traditional technologyLentikats BiotechnologyFermentation volume3,450 m3360 (2 x 180) m3Reserve reactor-180 m3Pre-fermentation volume213 m3Volume for preparation of nutrients16 m3Equalisation tank before distillationSanitation tank48 m3Total3,727 m3768 m3Production of ethanol39,035,667 L/year41,666,667 L/yearProcess yield290 L/tons309 L/tonsBiotechnologies are changing theworld…..
23Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biocatalyst typeLifetimeAnnual consumption of Lentikats BiocatalystPrice (€/year)immobilised yeast Saccharomyces cerevisiae8*–12* months61.5*–41** tons790,000 –526,500Biotechnologies are changing theworld…..* Guaranteed lifetime ** Assumed lifetimeBiotechnologies that are environmentally friendly …
24Profit of ethanol production at the capacity of 125,000 L a.a. per day Traditional technologyLentikats BiotechnologyEthanol production (m3/year a.a.)39,03641,667Ethanol yield from molasses (L/ton)290309Annual revenues for ethanol (€)24,600,00026,500,000Gross profit without depreciation (€)7,250,0008,185,000Biotechnologies are changing theworld…..Solution for higher efficiency and stability…
25Case study – distillery processing sugar solutions (sugar cane or sugar beet juice)
26Sugar distillery (125,000 L a.a. per day, continuous mode) 45 tons of Lentikats Biocatalystsugar solution41 667 m3/year a.a.140 g/L TFS ** Total Fermentable Sugars
28Economic comparison of traditional technology and Lentikats Biotechnology for distillery processing sugar solutions
29Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biocatalyst typeLifetimeAnnual consumption of Lentikats Biocatalyst *Price (€/year)immobilised bacteria Zymomonas mobilis1.5*–2** years27.4*–20.5** tons351,500–263,500Biotechnologies are changing theworld…..Biotechnologies are changing theworld…..* Guaranteed lifetime, ** assumed lifetimeBiotechnologies that areenvironmentally friendly …
30Profit of ethanol production at the capacity of 125,000 L a.a. per day Traditional technologyLentikats BiotechnologyEthanol production (m3/year a.a.)39,03641,667Annual revenues for ethanol (€)24,600,00026,500,000Gross profit without depreciation (€)7,240,0008,630,000Solution for higher efficiency and stability…
35Economic comparison of traditional technology and Lentikats Biotechnology for corn distillery
36Reactor volume reduction – reduced investments Traditional technologyLentikats BiotechnologyMixing tank14 m3Fermentation reactor26 m3Saccharification reactor9 m336 m3Fermentation volume552 m372 (2 x 36) m3Reserve reactor-Pre-fermentation volume86 m3Sanitation tankEqualisation tank before distillationTotal696 m3219 m3Production of ethanol4,473,667 L/year5,000,000 L/yearProcess yield344 L/tons385 L/tons
37Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biocatalyst typeLifetimeAnnual consumption of Lentikats Biocatalyst *Price (€/year)immobilised enzyme glucoamylase1,200*–1,500** hours11.9*–9.5**tons150,000 –120,000immobilised yeast Saccharomyces cerevisiae1.5*–2**years3.6*–2.7**46,000 –34,500* guaranteed lifetime, ** assumed lifetimeBiotechnologies that areenvironmentally friendly …
38Profit of ethanol production at the capacity of 15,000 L a.a. per day Traditional technologyLentikats BiotechnologyEthanol production (m3/year a.a.)4,4745,000Ethanol yield (L/ton of corn)344385Annual revenues for ethanol (€)2,817,0003,150,000Gross profit without depreciation (€)1,370,0001,600,000Solution for higher efficiency and stability…
39Other Lentikats Biotechnology Applications Lentikats Biocatalysts and applied Lentikats Biotechnology also deliver additional value and benefits in the following sectors :waste water treatment – nitrogen removalpharmaceutics – production of robust biocatalystsfood industry – whey processing, production of special sugars carbohydrates and syrupsBiotechnologies are changing the world…
41Lentikats Biotechnology services Monitoring of specified technological procedures and conditions.Periodical monitoring of Lentikats Biocatalyst’s activity.Supply and replacement of Lentikats Biocatalyst in such periods and volumes to sustain the required Lentikats Biotechnology parameters.Disposal of the used Biocatalyst.
42What can we offer to you?Have we raised your interest in testing LentikatsBiotechnology in your own project?We are prepared to supply a sufficient amount of our Biocatalyst and provide full assistance in order to convince you of the obvious advantages our technology has when applied in your distillery.For all companies introducing new technologies, the first applications and end results are the most important ones. That is why we offer excellent conditions to potential clients in an effort to eliminate any of their risks.
43Guarantee of your investments and the investment return You can test our biocatalyst easily by yourself with your substrate in your own plant or plant of your choice to see the results.We offer to you our assistance and full support at:- pilot model installation- installation into an existing reactorLoad of Biocatalyst for testing is free of charge.
45Our know-how, references and experiences Production of bioethanol from sugar beet molasses in Bioferm Kolín distillery with a capacity of 6 litres of absolute alcohol per hour.Processing of technical-technological documentation for the supply to an agricultural refinery with a production capacity of cca m3 of bioethanol per year, based on our concept of Bio Plant 2015.Processing of technical documentation for the delivery of a distillery with a production capacity of cca 450 hl of bioethanol per day utilising corn as a raw material.Production of bioethanol from starch milk of type B in Krnov distillery with a capacity of 12 litres of absolute alcohol per hour.Process optimisation and processing of technical documentation for a reconstruction of the existing distillery in India which uses cane molasses as a substrate.
46Our partners in the field of industrial applications Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) , Maharashtra, IndiaVÚCHZ, a.s., Křižíkova 70, Brno, CZGresham’s Eastern (Pvt) Ltd.F-302 Sind Industial Trading EstateKarachiZinchem, Námestie Slobody 5, Michalovce, SKskgeniaLab®, BioTechnologie-Produkte und Dienstleistungen Gmbh Büchnerstraße 7, Braunschweig, Germany46
47Our partners in the field of research and development ICT Prague, Department of Fermentation Chemistry and bioengineering, Technická 5, Praha 6, CZFermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) , Maharashtra, IndiaCHEMCOMEX Praha, a.s., Pražská 810/16, Praha 10, CZInstitute of Microbiology, Academy of Science of the Czech Republic Vídeňská 1083, Praha 4, Krč, CZSTU Bratislava, Faculty of chemical and food technology, Institute of biotechnology and food technology, Radlinského 9, Bratislava, SKAssociation of Alcohol Producers in Slovakia, Záhradnícka 21, Bratislava, SKSekab, Box 286, s Örnsköldsvik, Sweden47
48Thank you for attention Company Headquarters:LentiKat´s a.s.Evropska 423/178Prague 6Czech republicTel.:Fax:Production plant and laboratoriesPod Vinicí 83Stráž pod RalskemTel.:Fax:4848