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Lentikats Biotechnology in distilleries. Content 1. PRINCIPLES Principles of Lentikats Biotechnology Benefits of Lentikats Biotechnology Lentikats Biotechnology.

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Presentation on theme: "Lentikats Biotechnology in distilleries. Content 1. PRINCIPLES Principles of Lentikats Biotechnology Benefits of Lentikats Biotechnology Lentikats Biotechnology."— Presentation transcript:

1 Lentikats Biotechnology in distilleries

2 Content 1. PRINCIPLES Principles of Lentikats Biotechnology Benefits of Lentikats Biotechnology Lentikats Biotechnology in distilleries 2. CASE STUDIES Case study – molasses distillery Case study – distillery processing sugar solutions Case study – corn distillery 3. WHAT CAN WE OFFER? References and experiences, our partners Contacts

3 Principles of Lentikats Biotechnology

4 What is a Lentikats Biocatalyst? Unique shape of the matrix and the original principle of immobilisation enable the encapsulation of free enzymes or microorganisms (bacteria, yeasts, fungi) in a stable porous PVA (polyvinylalcohol) carrier. The carrier‘s advantages are: Excellent physio-mechanical characteristics that provide for long-term mechanical stability Low biodegradability Zero toxicity Lentikats Biocatalyst enables easy separation of enzymes and microorganisms from the surrounding media and therefore it can be reused multiple times. Biotechnologies are changing the world…

5 Lentikats Biocatalyst Advantages of using PVA as a matrix for encapsulation: inexpensive, non-toxic polymerization under optimal conditions, cell conservation enables creation of hydrogels with excellent mechanical properties no side effects in processes large capacity for cell building easily supervised processes stable processes easy separation from bulk media

6 Lentikats Lentikats Biocatalyst & Biotechnology (our product) (application process) Lentikats BiocatalystProduction Lentikats Biocatalyst Application

7 How does Lentikats Biocatalyst function? Due to its maximum surface and at the same time minimum volume of the Lentikats Biocatalyst, the diffusion limitations associated with the transport of the substrate into the lens and the product out of the lens are significantly reduced.

8 Production of Lentikats Biocatalyst

9 Lentikats Biocatalyst Production plant in Stráž pod Ralskem

10 Benefits of Lentikats Biotechnology

11 What are the advantages of Lentikats Biotechnology over traditional technologies? Higher biomass concentration Reaction rate Biomass concentration Reduced volume of reactors Investment costs saving Operational costs saving (electricity, heat, etc.) Higher yields 1) 100 € 11

12 What are the advantages of Lentikats Biotechnology over traditional technologies? Easy separation from bulk media Repeatable use of Biocatalyst Operational costs saving Lower production of waste and by-products 100 € Applicability under extreme or stress conditions 2) 3) Robustness towards environmental shocks profound change of pH level higher concentration of toxic compounds

13 Lentikats Biotechnology in distilleries

14 Technological process set-up Legend: 1.Sugars media inlet 2.Fermented media 3.Inlet for Lentikats Biocatalyst filling 4.Outlet for Lentikats Biocatalyst emptying 5.Sieve separator (angle slop 15-20°, aperture mm) 6.Liquid fermentation broth with Lentikats Biocatalyst 7.Liquid fermentation broth without Lentikats Biocatalyst 8.Gear with speed change box 9.Stirrer 10.pH probe well 11.Temperature probe well 12.Antifoam agent inlet 13.Liquid level measurement (stainless steel pressure probe)

15 Which substrates can be used for Lentikats Biotechnology? Lentikats Biocatalysts provide a solution for the production of bioethanol from the following substrates: 1.Molasses, beet juice and sugar cane 2.Starch-containing (cereal) materials (corn, wheat, etc.) 3.Ligno-cellulose containing materials (straw, pollard…)

16 Our products Lentikats Biocatalyst for the fermentation of sucrose, glucose and lactose solutions (immobilized bacteria Zymomonas mobilis, yeast Saccharomyces cerevisiae, Kluyveromyces sp.) Lentikats Biocatalyst for the 2nd step of hydrolyses of liquefied starch solution (immobilized enzyme glukoamylase) Lentikats Biocatalyst for the production of inverted sugar from molasses (immobilized enzyme invertase) Energy is all around us… …the key is to obtain it effectively… …and protect the environment at the same time…

17 β

18 Case study – molasses distillery

19 Molasses distillery (125,000 L A.A. per day, continuous mode) (TFS* 48 %) 404 tons/day * Total Fermentable Sugars 130 g/L TFS 45 tons of Lentikats Biocatalyst m 3 /year a.a.

20 Fermentation conditions Saccharomyces cerevisiae continuous mode 180 g sucrose. hour -1. kg -1 ethanol yield 95 % 8.4 vol. % ethanol

21 Biotechnologies are changing the world….. Economic comparison of traditional technology and Lentikats Biotechnology for molasses distillery

22 Biotechnologies are changing the world….. Reactor volume reduction – reduced investments Traditional technologyLentikats Biotechnology Fermentation volume3,450 m (2 x 180) m 3 Reserve reactor m 3 Pre-fermentation volume213 m 3 - Volume for preparation of nutrients16 m 3 - Equalisation tank before distillation m 3 Sanitation tank48 m 3 Total 3,727 m m 3 Production of ethanol39,035,667 L/year41,666,667 L/year Process yield290 L/tons309 L/tons

23 Biotechnologies are changing the world….. Lentikats Biocatalyst consumption (125,000 L a.a. per day) * Guaranteed lifetime ** Assumed lifetime Biocatalyst typeLifetime Annual consumption of Lentikats Biocatalyst Price (€/year) immobilised yeast Saccharomyces cerevisiae 8*–12* months 61.5*–41** tons 790,000 –526,500 Biotechnologies that are environmentally friendly …

24 Biotechnologies are changing the world….. Profit of ethanol production at the capacity of 125,000 L a.a. per day Traditional technologyLentikats Biotechnology Ethanol production (m 3 /year a.a.) 39,03641,667 Ethanol yield from molasses (L/ton) Annual revenues for ethanol (€) 24,600,00026,500,000 Gross profit without depreciation (€) 7,250,0008,185,000 Solution for higher efficiency and stability…

25 Case study – distillery processing sugar solutions (sugar cane or sugar beet juice)

26 Sugar distillery (125,000 L a.a. per day, continuous mode) sugar solution 45 tons of Lentikats Biocatalyst 140 g/L TFS * * Total Fermentable Sugars m 3 /year a.a.

27 Biotechnologies are changing the world….. Biotechnologies are changing the world….. Fermentation conditions Zymomonas mobilis continuous mode 180g saccharose. hour -1. kg -1 temperature: 30–35°C, pH 4.8–5 ethanol yield 95 % 9.02 vol. % ethanol

28 Economic comparison of traditional technology and Lentikats Biotechnology for distillery processing sugar solutions

29 Biotechnologies are changing the world….. Biotechnologies are changing the world….. * Guaranteed lifetime, ** assumed lifetime Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biocatalyst typeLifetime Annual consumption of Lentikats Biocatalyst * Price (€/year) immobilised bacteria Zymomonas mobilis 1.5*–2** years 27.4*–20.5** tons 351,500 –263,500 Biotechnologies that are environmentally friendly …

30 Profit of ethanol production at the capacity of 125,000 L a.a. per day Traditional technologyLentikats Biotechnology Ethanol production (m 3 /year a.a.) 39,03641,667 Annual revenues for ethanol (€) 24,600,00026,500,000 Gross profit without depreciation (€) 7,240,0008,630,000 Solution for higher efficiency and stability…

31 Case study – corn distillery

32 Corn distillery (15,000 L a.a. per day, continuous mode) (60 % of starch) 39 tons/day 1.8 tons of LB 6 tons of LB 5,000,000 m 3 /year a.a.

33 Hydrolysis conditions glucoamylase continuous mode 600 g glucose. hod -1. kg -1 temperature: 38–40°C, pH 4.5–5 conversion rate 95 % outlet concentration 150 g/L

34 Biotechnologies are changing the world….. Biotechnologies are changing the world….. Fermentation conditions Zymomonas mobilis continuous mode 180g glucose. hour -1. kg -1 temperature: 30–35°C, pH 4.8–5 ethanol yield 95 % 9.21 vol. % ethanol

35 Economic comparison of traditional technology and Lentikats Biotechnology for corn distillery

36 Reactor volume reduction – reduced investments Traditional technologyLentikats Biotechnology Mixing tank14 m 3 Fermentation reactor26 m 3 Saccharification reactor9 m 3 36 m 3 Fermentation volume552 m 3 72 (2 x 36) m 3 Reserve reactor-36 m 3 Pre-fermentation volume86 m 3 - Sanitation tank9 m 3 Equalisation tank before distillation-26 m 3 Total696 m m 3 Production of ethanol4,473,667 L/year5,000,000 L/year Process yield344 L/tons385 L/tons

37 Lentikats Biocatalyst consumption (125,000 L a.a. per day) Biocatalyst typeLifetime Annual consumption of Lentikats Biocatalyst * Price (€/year) immobilised enzyme glucoamylase 1,200*–1,500** hours 11.9*–9.5** tons 150,000 –120,000 immobilised yeast Saccharomyces cerevisiae 1.5*–2** years 3.6*–2.7** tons 46,000 –34,500 Biotechnologies that are environmentally friendly … * guaranteed lifetime, ** assumed lifetime

38 Profit of ethanol production at the capacity of 15,000 L a.a. per day Traditional technologyLentikats Biotechnology Ethanol production (m 3 /year a.a.) 4,4745,000 Ethanol yield (L/ton of corn) Annual revenues for ethanol (€) 2,817,0003,150,000 Gross profit without depreciation (€) 1,370,0001,600,000 Solution for higher efficiency and stability…

39 Other Lentikats Biotechnology Applications Lentikats Biocatalysts and applied Lentikats Biotechnology also deliver additional value and benefits in the following sectors : waste water treatment – nitrogen removal pharmaceutics – production of robust biocatalysts food industry – whey processing, production of special sugars carbohydrates and syrups Biotechnologies are changing the world…

40 What can we offer?

41 Lentikats Biotechnology services Monitoring of specified technological procedures and conditions. Periodical monitoring of Lentikats Biocatalyst’s activity. Supply and replacement of Lentikats Biocatalyst in such periods and volumes to sustain the required Lentikats Biotechnology parameters. Disposal of the used Biocatalyst.

42 Have we raised your interest in testing Lentikats Biotechnology in your own project? We are prepared to supply a sufficient amount of our Biocatalyst and provide full assistance in order to convince you of the obvious advantages our technology has when applied in your distillery. For all companies introducing new technologies, the first applications and end results are the most important ones. That is why we offer excellent conditions to potential clients in an effort to eliminate any of their risks. What can we offer to you?

43 Guarantee of your investments and the investment return You can test our biocatalyst easily by yourself with your substrate in your own plant or plant of your choice to see the results. We offer to you our assistance and full support at: - pilot model installation - installation into an existing reactor Load of Biocatalyst for testing is free of charge.

44 References and experiences, our partners

45 Our know-how, references and experiences 1.Production of bioethanol from sugar beet molasses in Bioferm Kolín distillery with a capacity of 6 litres of absolute alcohol per hour. 2.Processing of technical-technological documentation for the supply to an agricultural refinery with a production capacity of cca m 3 of bioethanol per year, based on our concept of Bio Plant Processing of technical documentation for the delivery of a distillery with a production capacity of cca 450 hl of bioethanol per day utilising corn as a raw material. 4.Production of bioethanol from starch milk of type B in Krnov distillery with a capacity of 12 litres of absolute alcohol per hour. 5.Process optimisation and processing of technical documentation for a reconstruction of the existing distillery in India which uses cane molasses as a substrate.

46 Our partners in the field of industrial applications Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) , Maharashtra, India VÚCHZ, a.s., Křižíkova 70, Brno, CZ Gresham’s Eastern (Pvt) Ltd. F-302 Sind Industial Trading Estate Karachi Zinchem, Námestie Slobody 5, Michalovce, SK sk geniaLab®, BioTechnologie-Produkte und Dienstleistungen Gmbh Büchnerstraße 7, Braunschweig, Germany

47 Our partners in the field of research and development ICT Prague, Department of Fermentation Chemistry and bioengineering, Technická 5, Praha 6, CZ Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) , Maharashtra, India om CHEMCOMEX Praha, a.s., Pražská 810/16, Praha 10, CZ Institute of Microbiology, Academy of Science of the Czech Republic Vídeňská 1083, Praha 4, Krč, CZ STU Bratislava, Faculty of chemical and food technology, Institute of biotechnology and food technology, Radlinského 9, Bratislava, SK Association of Alcohol Producers in Slovakia, Záhradnícka 21, Bratislava, SK p?zvez=zvll Sekab, Box 286, s Örnsköldsvik, Sweden

48 Thank you for attention Company Headquarters: LentiKat´s a.s. Evropska 423/ Prague 6 Czech republic Tel.: Fax: Production plant and laboratories LentiKat´s a.s. Pod Vinicí Stráž pod Ralskem Czech republic Tel.: Fax:


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