Presentation on theme: "Home detergents and DNA extraction from fruit"— Presentation transcript:
1 Home detergents and DNA extraction from fruit Dana Hogan
2 ProblemTesting which home detergent- shampoo, laundry detergent, or dishwashing detergent- most effectively extracts DNA from kiwi
3 ResearchDNA, or deoxyribonucleic acid, is the genetic material for all living things that can be found in the cell nucleus.DNA extraction is the removal of deoxyribonucleic acid from the cells or viruses in which it can normally be found.In a basic DNA extraction, the steps included: lyses of tissue and/or cells, precipitating the DNA, the application of a detergent, and heat denaturation.The kiwifruit, or Actinidia deliciosa, is made-up mainly of sugar and ascorbic acid.A surfactant, a main component of cleaning detergents, is a substance that has the ability to remove dirt, grease, or oil from other surfaces when it is added to water.Previous student studies on the subject of the effectiveness of home detergents and DNA extraction from fruit found that laundry detergent proved the most effective.
4 HypothesisIf the effectiveness of shampoo, laundry detergent, and dishwashing detergent in DNA extraction from fruit were tested, then dishwashing detergent would prove the most effective.
5 Materials pH paper 8g salt Glass stirring rod 200mL cold ethanol 500mL beakerSmall metal hookVolumetric flask100mL graduated cylinderWeighing paperFreezer6 200mL beakersBalance weighing to .01gHot water bathBalance weighing to .001gThermometer2 large Tupperware containersSmall blenderFork and knifeIce480g mashed kiwiCheesecloth20mL Suave® shampooCoffee filters20mL all® laundry detergent6 1000mL beakersFilter20mL Seventh Generation™ dishwashing detergentStrainer10mL graduated cylinder360mL distilled water20 test tubes
6 Procedure PH balance of detergents was tested. A salt-water solution of 180mL of water and 4g of salt was prepared.60g mashed kiwi were poured into a 200mL beaker and researcher 10mL detergent and 30g salt-water solution were added.Mixture heated in hot water bath at 50ºC for 10 minutes, stirring occasionally.Mixture transferred to ice bath for 5 minutes.Cooled mixture was filtered into 1000ml beaker. (In first set of trials, cheesecloth was used for filtration, whereas in the second set of trials, coffee filters were used for filtration.)Once 15mL of filtrated were gathered, 3 test tubes with 5mL of filtrate each were filled.10mL of cold ethanol were carefully poured into each test tube.Test tubes placed in ice bath for 5 minutes.Spooling of visible DNA was attempted.Spooled DNA was spread onto pre-weighed weighing paper and let dry for 2 days.Mass of DNA extracted was calculated.This procedure was repeated twice for shampoo and dishwashing detergent samples. In the first set of trials, 1 control sample was tested, but in the second set of trials, 3 control samples were tested. In first set of trials, 3 samples of laundry detergent were tested, but in the second set, 1 sample was tested.
7 Variables Independent variable- type of detergent added Dependent variable- amount of DNA extractedControl- samples without detergentConstants- amount of salt-water, kiwi, and detergent added, temperature during incubation, length of time allotted for incubation and cooling, amount of filtrate used, amount of ethanol added to filtrate
8 Data DNA Extracted from Each Sample (g) Control Shampoo Laundry Dish (1)Dish (2)Trail 10.010.124Trial 20.020.099Trial 30.095Average0.106
11 ConclusionThe purpose of this experiment was to see which home detergent- shampoo, laundry detergent, or dishwashing detergent- would most effectively extract DNA from fruit.The hypothesis that if the effectiveness of shampoo, laundry detergent, and dishwashing detergent in DNA extraction from fruit were tested, then dishwashing detergent would prove the most effective was supported.Knowledge gained through this experiment can help find more cost effective methods of DNA extraction for various purposes, whether it is in schools or advanced laboratories.
12 DiscussionPossible errors that occurred during experimentation include different filtration methods, varying methods for mashing the kiwi, and the temperature of the ethanol.If repeated, more samples would be tested, coffee filters would consistently be used as the method of filtration, a balance measuring to .001 would be consistently used, and the ethanol would remain in the freezer up until it was needed.Further experimentation could include testing different types of fruits and organic vs. nonorganic detergents.
13 ReferencesBachor, K. (n.d.). The best detergent for plentiful DNA extraction. Retrieved fromBerlow, L. H. (2011). Laundry detergent. Retrieved from eNotes.com, Inc. website:Morton, J. F. (1987). Fruits of warm climates (pp ). Retrieved fromOmbrello, T. (n.d.). The Pineapple. Retrieved fromRice, G. (2010, October 28). DNA extraction. Retrieved fromStrawberry. (2011). In Britannica. Retrieved fromSurfactants. (2005). Retrieved from Procter&Gamble website:Understanding automatic dishwashing. (2010). Retrieved from American Cleaning Institute website:What is DNA? (2011, October 4). Retrieved from US National Library of Medicine website:What is hair shampoo? (2011). Retrieved from Herbal Luxuries Natural Skin Care & Acne Products website:World of Forensic Science. (2005). DNA isolation methods. Retrieved from Encylcopedia.com website: