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OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID vs. DIME.

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Presentation on theme: "OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID vs. DIME."— Presentation transcript:

1 OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID vs. DIME

2 OU NanoLab/NSF NUE/Bumm & Johnson How do they look under microscope? Hexagonal Grid Square Grid 200 mesh 55% open area, 28 µm bar width Thick:17.8 µm 3 mm

3 OU NanoLab/NSF NUE/Bumm & Johnson TEM Grid Preparation There are two sides in carbon coated ultra- thin grid 1.Formvar side - lighter side (less hydrophobic than the carbon film) 2.Carbon film is on the darker side.

4 OU NanoLab/NSF NUE/Bumm & Johnson TEM Grid Preparation Hold the grid with tweezers. Do not touch/contaminate the grid. Do not bend the grid.

5 OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID PREPARATION Take a micropipette and put a droplet on top the grid. Let the solution sit on the grid. After min, wick away the rest of the solution with filter paper. Do not touch the grid! Finally let it sit until the grid dries off.


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