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OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID vs. DIME.

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Presentation on theme: "OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID vs. DIME."— Presentation transcript:

1 OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID vs. DIME

2 OU NanoLab/NSF NUE/Bumm & Johnson How do they look under microscope? Hexagonal Grid Square Grid 200 mesh 55% open area, 28 µm bar width Thick:17.8 µm 3 mm

3 OU NanoLab/NSF NUE/Bumm & Johnson TEM Grid Preparation There are two sides in carbon coated ultra- thin grid 1.Formvar side - lighter side (less hydrophobic than the carbon film) 2.Carbon film is on the darker side.

4 OU NanoLab/NSF NUE/Bumm & Johnson TEM Grid Preparation Hold the grid with tweezers. Do not touch/contaminate the grid. Do not bend the grid.

5 OU NanoLab/NSF NUE/Bumm & Johnson TEM GRID PREPARATION Take a micropipette and put a droplet on top the grid. Let the solution sit on the grid. After 10-12 min, wick away the rest of the solution with filter paper. Do not touch the grid! Finally let it sit until the grid dries off.


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