2 GENE EXPRESSION - How the genes are expressed is a function of the protein produced. DNA (gene)RibosometRNA & rRNAProteinm RNATrait (expression)
3 There are two processes that make this happen… TranscriptionTranslationmRNA strand is built from the code on the DNA strand (gene)mRNA strand is used at the ribosome to help join amino acids to form a protein.
5 Requirements for Transcription a gene segment on the DNAmany free floating RNA nucleotidesthe enzyme RNA polymerasePREDICT: What do you think the RNA polymerase will do?
6 Steps in transcription 1DNA double helix unwinds at the area of the DNA where the gene is located2Weak hydrogen bonds break between the 2 DNA strands3Free floating RNA nucleotides bond with complimentary DNA nucleotides to form a strand of mRNA4When the mRNA is completely formed, it breaks away from the DNA. The DNA strands reconnect and the mRNA is free to travel to the cytoplasmThese stages are catalyzed by the enzyme RNA polymerase
7 Each triplet code on a DNA molecule is transcribed into a triplet codon on the mRNA molecule. If the DNA codes for a polypeptide isT-A-C—C-C-G—T-A-G—C-T-T—A-C-TWhat would the codons on the complimentary strand of mRNA codons look like?A-U-G – G-G-C – A-U-C – G-A-A – U-G-ADNA codes:T-A-C—C-A-T—C-C-C—A-A-A—A-C-TmRNA codons:A-U-G – G-U-A – G-G-G – U-U-U – U-G-A
8 Think…think…..think….What does each letter in the code or codon stand for represent?That’s right….a nucleotideWhat do the particular codes and codons represent?Amino Acids….good job!Because each base triplet on the mRNA stands for an amino acid, each mRNA molecule must contain how many times the number of nucleotides as the number of amino acids making up the protein product.3X….Wow! You guys are on a roll!
9 Engage your BRAIN….The intersection of science and math An mRNA strand with 66 nucleotides codes for a polypeptide how many amino acids in length?22…you all are Math whizzesA protein 300 amino acid units in length was synthesized from an mRNA strand how many nucleotides in length?900…Woo Hoo!A DNA strand 700 nucleotides in length will be transcribed into an mRNA strand how many nucleotides in length.700…Ah! This one is tricky!
10 How do we read mRNA codons? There are 4 different bases in DNA (A, C, G, T) which can combine in different triplets to form 64 possible triplet codes (43).When these codes are transcribed into mRNA, there are 64 triplet codons which can be formed.Only 20 amino acids are used so sometimes more than one codon represents an amino acid.We can use the chart to the right to determine the exact sequence of amino acids that will make up a protein.
11 different codons (using A, G, C and U in triplets) How many different mRNA codons are there on the chart?43 = 4 x 4 x 4 = 64different codons (using A, G, C and U in triplets)How many different amino acids are there?Only 20Which amino acids only have ONE triplet code assigned to them?Methionine and TrytophanWith the exception of Methionine and Tryptophan,more than one nucleotide triplet codes for each amino acid.Give an example of an amino acid which has more than one codon.valine, alanine, isoleucine, threonine, and many moreWhat do you notice about the codons that code for the same amino acid?They are very similar and usually only the last nitrogen base is different.
12 Special Codons Start Codon AUG-codes for the amino acid methionine. It signals the ribosome that it is the beginning of the mRNA sequence.Stop CodonsUAA, UAG, UGA- Don’t code for an amino acid!!!! Signal the ribosome that it is the end of the mRNA sequence and triggers the release of the mRNA from the ribosome.
13 What is the DNA code for the mRNA start codon, methionine? TACWhat are the DNA codes for the stop codons UAA, UAG and UGA?ATT, ATC, ACTAfter transcription has taken place, the mRNA moves out of the nucleus to link with a ribosome in the cytoplasm. Translation will take place here…..
15 Requirements for Translation m RNA carries the DNA code as “codons”ribosomes 2 rRNA+ protein subunits--site of protein synthesistRNA carry the amino acids from the cytoplasm to the ribosome to be joined to form the proteinAbundant amino acids in the cytoplasm and all necessary enzymes
16 Steps in TranslationmRNA joins with the ribosome (in cytoplasm or near the Rough Endoplasmic ReticulumtRNA (with an amino acid attached) joins the mRNA codon to ANTI-codon (The anticodon on the tRNA is complimentary to the codon on the mRNA.)a second tRNA with attached amino acid joins the mRNAa peptide bond forms between the first two amino acidsthe first tRNA is released from the mRNA amino acids stay bonded to each otherthe mRNA slides through the ribosome to expose the next codonthe next tRNA with amino acid joins the mRNASequence is repeated Repeat steps 4-8 until a stop codon is reachedmRNA and protein is released from the ribosome
19 The Final Products of Protein Synthesis protein or polypeptide: functional or non-functional sequence of amino acidsMany times a protein must be modified after it is translated. This is done in the cytoplasm, ER, GolgiSome proteins are coded for by a single gene. For example Insulin (later broken into two segments)Sometimes proteins are composed of different genes hemoglobin (2 genes/4 segments)The difference between different proteins is in the number, types and sequences of amino acids.
20 Let’s Watch a video to sum up Transcription and Translation…