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“ Sushi-gate 2008”: High School students apply DNA barcoding to fish sold in their NYC neighborhood, discover one-quarter is mislabeled Research report.

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Presentation on theme: "“ Sushi-gate 2008”: High School students apply DNA barcoding to fish sold in their NYC neighborhood, discover one-quarter is mislabeled Research report."— Presentation transcript:

1 “ Sushi-gate 2008”: High School students apply DNA barcoding to fish sold in their NYC neighborhood, discover one-quarter is mislabeled Research report by students Kate Stoeckle and Louisa Strauss published in Pacific Fishing September 2008 Can DNA name this fish?

2 The New York Times August 21, 2008, page 1 Chosun Ilbo (Korean Daily News) circulation 2.2 million “Sushi-gate” draws wide interest CBS Early Show August 23, 2008

3 Barcode of Life Database (BOLD): BOLD project “Fish Market Survey” Analyzing Sushi-gate fish samples DNA sequencing at University of Guelph   1. Collect specimens2. Prepare samples3. Record data4. Send for analysis 5. Upload specimen, sequence data on BOLD 5. Search databases for identical or closely-related sequences 6. Learn more on FishBase, other  

4 Mislabelings were as more expensive or more desirable fish “Red Snapper” DNA ID: Nile PerchRed Snapper to scale Among 60 items tested: -Nile Perch (Africa), Lavender Jobfish (SE Asia), Slender Pinjalo (SE Asia), and Acadian Redfish (N Atlantic) sold as “Red Snapper” -Mozambique Tilapia sold as “White Tuna” -Smelt Roe sold as “Flying Fish Roe” -Caribbean Spotted Goatfish sold as “Mediterranean Red Mullet” -White Bass (farmed freshwater fish) sold as “Sea Bass” Mislabeled items were sold in 6 of 10 grocery stores/fish markets and 2 of 3 restaurants Range map: FishBase FishBase: D FlescherFishBase: John Casselman

5 The Trinity School Kate Stoeckle Louisa Strauss The Rockefeller University Jesse Ausubel Mark Stoeckle University of Guelph Robert Hanner Eugene Wong Fish Barcode of Life Initiative Unexplored territory? DNAHouse: 2009 student project exploring urban environment with DNA barcoding ?? Acknowledgments


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