Presentation on theme: "OUTLINE OF PRESENTATION Phenylpropanoid/avenanthramide biosynthesis Health benefits of avenanthramides Application of suspension cultures to investigate."— Presentation transcript:
OUTLINE OF PRESENTATION Phenylpropanoid/avenanthramide biosynthesis Health benefits of avenanthramides Application of suspension cultures to investigate avenanthramide biosynthesis
General Phenylpropaniod Pathway + ProteinTryptophan/protein PAL
Reaction of Phenylpropanoid and 5- Hydroxyanthranilic Acid Catalyzed by HHT Form R phenylpropanoid A (Bp) H p-Coumaric B (Bf) OH caffeic C (Bc) OCH 3 ferulate
Effect of Avenanthramide C on VSMC Proliferation [ 3 H] thymidne incorporation (% of control) Nie, Wise, Peterson, Meydani, Atherosclerosis, 186, (2006).
Avn C inhibition of Restinoblastosis protein (pp-Rb) polyphosphorylation in VSMC Nie, Wise, Peterson, Meydani, Free Rad. Biol. Med, in press
Effect of Dietary Avenanthramide C on Exercise Stressed Rats Reduction of oxidized glutathione levels and lower ratio of reduced to oxidized form of glutathione. O’Moore and Ji, (2006) unpublished results.
Summary of Health Effects Reduces vascular smooth muscle cell proliferation Increased NO production Inhibition of ppRb, arrest G1-S1 cell cycle progression Elevation in anti-oxidant enzyme system in rats
Oat (cv Belle) Suspension Cultures
HPLC Trace of Media Extract Subsequent to Chitin Elicitation
Avenanthramide A in Culture Media After Elicitation with Chitin
Avenanthramide A in Tissue Extracts Subsequent to Chitin Elicitation
Northern Analysis of Suspension Cells for Barley PR-1 and HHT Subsequent to Elicitation 18S RNA bPR-1 HHT
HHT Enzyme Activity Post Induction
Conclusions/Future Directions Avenanthramides are a “value added” phytonutrient unique to oat Avn content in the grain is highly variable Basis for variability is unknown Suspension cultures offer a model to study avenanthramide biosynthesis at the molecular level SSH cDNA library prepared What is the involvement of the various isozymes (HHT, 4-CL, PAL)?
ACKNOWLEDGEMENTS Jean Mayer Human Nutrition Research Center MOHSEN MEYDANI LIN NIE Univ Wisconsin, Dept Kinesiology LI LI JI KATHLEEN O’MOORE Cereal Crops Research RACHEL ANGEL LAURI HERRIN NICKOLAS PARTRIDGE RON SKADSEN JOHN HERBST