1. Using Stable δ 15 N Ratios to Examine Sources of Nitrogen within a Residential Community Nora Egan Demers Department of Biological Sciences, Florida Gulf Coast University, Ft. Myers, Florida, USA Abstract I report on a spatial study of stable nitrogen isotope ratios in the residential community of San Carlos Park, Lee County, FL, USA focusing on determining the influences of various anthropogenic nutrients (mainly septic and fertilizer) to the outstanding Florida waterway that leads to the Estero Bay. The study benchmarks the water quality of the storm-water drainage system in this community of nearly 8000 single-family and duplex homes (developed before most storm-water treatment requirements). The work is the first step in a multi-year restoration effort undertaken in partnership with local government, civic organizations and other participants. This project occurred during the summer and fall of 2007, and early spring of 2008. Results support using stable nitrogen isotopes to help distinguish anthropogenic sources of nitrogen pollutants, but not on the spatial scale of individual lots. I am grateful to the Watershed Council of Southwest Florida for funds and to the Lee County Environmental Lab for water sample testing (data not shown) for this study CHNEP Watershed Summit 2011 San Carlos Park (approximately 2,366 acres) (Section 46, Township 25, Range 08) single-family, residential community constructed in the 1960’s, prior to current storm-water treatment requirements. storm-water runoff from the San Carlos Park community is funneled to a single outflow system (Lee County monitoring site 46B-9GR), into the Mullock Creek headwaters. Mullock Creek is on the Florida Department of Environmental Protection’s impaired waters list for dissolved oxygen and Chlorophyll a. Mullock Creek flows directly into Estero Bay, an Outstanding Florida Water, west of U.S. 41. Figure 1. Aerial image showing surface drainage and water flow patterns for San Carlos Park, Lee County Florida. Approximate locations of homes with septic tanks are indicated by blue highlighting. Aerial image showing surface drainage and water flow patterns for San Carlos Park, Lee County Florida. Approximate locations of homes with septic tanks are indicated by blue highlighting. Canal sampling site at NE corner of community Lake at Golf course that takes effluent from water treatment facility- note green hue to water Stable nitrogen isotope research has been firmly established as an effective means to distinguish the various sources of nitrate contaminants in terrestrial soils and aquatic environments through examination of water, sediments, plants, and various consumers. Stable isotope research is based on the fact that the ratios of 14 N and 15 N occur overall on earth in a stable and fixed proportion of approximately 273 14 N atoms for each 15 N atom, (>99.6% 14 N) while the δ 15 N ratio among specific pools (wastewater, fertilizer and atmospheric nitrogen) in the environment differs (Peterson and Fry 1987). δ 15 N =[R (sample) -R (standard) /R (standard) ]*1000 where R= ratio of 15 N/ 14 N and R standard is the atmospheric standard. Generally, Nitrogen from fertilizers has an δ 15 N around 0+- 4 ppt (parts per thousand) (Heaton, 1986), septic systems and animal waste has a δ 15 N from +8 to +22 ppt. (Heaton, 1986). atmospheric depositions are about +2 to +8.00 ppt (Kreitler et al, 1978). Conclusion Enrichment of δ 15 N is highest (as expected) in the lake on the golf course that accepts wastewater from the local sewer treatment facility. Stable nitrogen isotopes are effective in distinguishing the sources of nitrogen, not on a spatial scale influenced by septic tanks in yards, but rather by cumulative flow. Future work will examine additional spatial and temporal aspects of the isotope ratios in this community to assess how much of the cumulative impact of enrichment of δ 15 N is from home septic versus the golf course lake effluent. References Cited Heatonn, T.H.E. (1986) Isotopic studies of nitrogen pollution in the hydrosphere and atmosphere: a review. Chemical Geology 59:87-102. Kreitler, C.W., S.E. Ragone and B.G. Katz (1978) 15N/14N ratios of ground-water nitrate, Long Island, New York. Ground water 16:404- 409. Peterson B.J., and B. Fry (1987) Stable isotopes in ecosystem studies. Annual Review Ecology Systematics 18:293-320. Acknowledgements To determine the efficacy of stable nitrogen isotopes (δ 15 N) in monitoring and understanding sources of nitrogen in a suburban community that primarily utilizes septic tanks for sewage treatment. Samples of sediments were obtained from various locations within this heavily urbanized community. They were compared to water quality and samples obtained at other sampling sites in the county which exemplify agricultural drainage, relatively pristine, and residential communities with central sewer. Samples were dried in an oven for a minimum of 48 hours and then small samples were processed and sent to University of California-Davis Stable isotope facility for analysis. Purpose: Methods: Contributions to excess nutrients include the apparently innate American desire for green lawns causes us to add too many nutrients and herbicides to our lawns, the excess of which is being transported into the failing storm-water system, and over 85% of the residences use septic tanks. A confounding factor is that the local community golf course receives the treated wastewater from the wastewater treatment plant and uses that water to irrigate the golf course- (to the tune of greater than 50,000 gallons per day,) a common practice in our region. Sediment samples stable isotope ratios location δ 15 N Bird bath algae-1.54 Lake surrounded by homes on sewer in Park 1.05 "Pristine" creek (Telegraph) 1.17 NE corner of Park (septic tank area) 1.87 Behind home with septic on side canal 2.5 residential community canal with no septic influence 2.69 Agricultural creek (Spanish Creek) 4.11 main flowway downstream of golf course 5.07 main flowway downstream of golf course- nearby homes on city sewer 5.37 outfall of community7.35 Golf course lake- no effluent 7.81 Golf Course effluent lake17.8 Littoral Islands placed to help remove nutrients from San Carlos Park community