1. Items due this week:  Friday of last week: CH 5 Questions  Today: Radish seed lab  Tuesday: origins of life notes (today)  Wednesday: reading notes unit 2 (CH 2, 3, 4,)  Wednesday/Thursday: Unit 2 TEST!  Friday: Cell Factory

2. Lipid Membrane, Diffusion, and Osmosis Unit 3 Flow

3. Reading Notes  Complete reading notes for CH 6, 7, 8, 25.1 and 25.3  Test is scheduled for 10/21 and 10/22  Reading notes are due on that day.

4. Day 1  Cell Factory Assignment  Complete the cell factory analogy to remember the cell organelle parts.  Or complete cell parts Handout using 3 cell ppts

5. Day 2  Origins of life and cells powerpoint  Take notes! Worth points

6. Day 3 – Coacervates Lab – Guided Activity  Read the Background information and answer the two pre-lab questions  Perform the Guided Activity (page 2, front and back)  Make sure you “measure” the coacervates you created.  Make sure you note the magnification you are using  Start the Inquiry Activity Part A  What will you vary to see if coacervates still form? Base? More Acid? Surface Tension? Salt content?

7. Day 4 – Coacervates Lab - Inquiry  Complete the “Inquiry” portion of the lab, Part B.  Complete all sections of the lab. Due Monday 10/6

8. Day 5 – Coacervates Lab - Shareout  Briefly present to the class what you did for your inquiry and what results you had.

9. Day 6 – Notes!  Take Cornell notes on the ppt: Cell Membrane and Diffusion  HMWK: Read through Chapter 7 in your text book. Write a paragraph explaining how the process of diffusion works. Describe some biological processes for which diffusion is a necessary phenomenon. To which of the 4 Big Ideas (Google Search “4 Big Ideas AP Bio”) does the process of diffusion relate. Finally create an illustration that explains diffusion

10. Day 7 – Finish Notes!  HMWK: Watch the Bozeman AP Biology lab video on diffusion:  http://www.bozemanscience.com/ap-bio-lab-1- diffusion-osmosis http://www.bozemanscience.com/ap-bio-lab-1- diffusion-osmosis  Complete a 3-2-1 on the video  3 items you learned from the video  2 questions you have  1 thing that you don’t understand

11. Day 8 – AP Labs Diffusion and Osmosis Day A  In this experiment, you will create models of living cells using dialysis tubing. Like cell membranes, dialysis tubing is made from a material that is selectively permeable to water and some solutes. You will fill your model cells with different solutions and determine where/if diffusion occurred.

12. Day 9 – Discussion of Day A  Record your results from yesterday’s lab  Answer all questions.  Handout due today!  Clean up:  Throw away your “cells”  Rinse all beakers thoroughly and set on tray to dry

13. Day 10 – AP Labs Diffusion and Osmosis Day B  In this experiment, you will create models of living cells using dialysis tubing. Like cell membranes, dialysis tubing is made from a material that is selectively permeable to water and some solutes. You will create two cells, one filled the opposite of the other  Cell #1Inside: sugar Outside: water  Cell #2 Inside: waterOutside: sugar  The cells will sit overnight and you will use mass to determine if OSMOSIS has occurred.

14. Day 11 – Discussion of Day B  Record your results from yesterday’s lab  Answer all questions.  Handout due today!  Clean up:  Throw away your “cells”  Rinse all beakers thoroughly and set on tray to dry

15. Day 12 – AP Labs Diffusion and Osmosis Day C  Use the computer and internet to complete Day C.  Links are on my website  Review of terms: http://www.youtube.com/watch?v=s3tEgNPox3g  Plasmolysis in red onion cell-with explanation http://www.youtube.com/watch?v=Sw87Q53NZLI  Plasmolysis in elodea cell-no explanation http://www.youtube.com/watch?v=SooSsKkJo1o

16. Water Potential – aka “Osmotic Pressure”  The tendency of water to leave one place in favor of another place.  Water ALWAYS moves from an area of higher water potential to an area of lower water potential.  In animals….water moves into or out of a cell depends on solute concentration on either side of the cell membrane ( cells lyse or shrink)  In plants…..Affected by two factors because of cell wall:  Pressure exerted by cell wall on cell membrane (think balloon trapped in a box)  Amount of solute (ions)  Formula for water potential is: Day 13

17.  In plants, pressure exerted by the rigid cell wall that limits further water uptake (also known as TURGOR PRESSURE)  Pure water has solute potential of ZERO. As solute is added, the value for solute potential becomes more negative. This causes the water potential to decrease also.  As solute is added, the water potential of a solution drops, and water will tend to move into the solution.  In the laboratory, we use “bars” as the unit of measure for water potential.  1 bar = approximately 1 atm

18. Examples:  Cell in distilled water.  Water potential outside = 0  Water potential inside = (-) since cells contain solutes which have a negative solute potential  Water will move into the cell since inside cell is more negative  Over time, the inflow of water into the cell causes pressure to build (positive turgor or pressure) which will eventually override the negative from the solute potential.  Eventually the water potential inside and outside the cell will equal each other and net water movement ceases  Note, I said “net” water movement. Equilibrium still means movement is occurring. It just means the water going in equals the water moving out of the cell

19. Day 13 (cont’d) – AP Lab Diffusion and Osmosis Day D  Design an experiment to identify the Molar (M) concentrations of the sucrose solutions and use the solutions to determine the water potential of the plant tissue.

20. Day 13 (cont’d) – AP Lab Diffusion and Osmosis Day D  Procedures:  1. Cut four (4) potato cubes (DO NOT include skin).  2. Mass your four cubes (1 set) for Initial weight.  3. Place cubes in cups with sucrose solution molarity. Label cups  4. Make observations and place cups in order of Molarity.  5. Let it stand overnight (24hrs)  6. Mass your four cubes for Final weight. Calculate % change in mass.  7. Construct a Graph with class data

21. Day 14 – AP Labs Diffusion and Osmosis Day D  Perform your lab  Answer all questions  Due 10/21

22. Day 15 – AP Lab Diffusion and Osmosis Day D…results  Record your lab results  Answer all parts of the lab  Due 10/21  Clean up:  Throw away potato pieces (not in sink!!!!)  Rinse all beakers thoroughly and set to dry on tray

23. Write a paragraph to answer the following:  Why are cells so small? And why do they have cell membranes with so many convolutions?

24. Day 16 – Enzyme notes  CH 8 – Enzymes  Take notes on the powerpoint. Will be worth points

25. Day 16 – Enzyme lab  Catalase

27. Day 5 – Internet Search  Research information about cell membranes (aka the lipid bilayer). This is a “fact-finding mission”. Your quest is to discover and document information (research, structure, function, et…anything related to the cell membrane) about the cell membrane.  You will compile your findings (from today’s research) along with important websites – on a piece of binder paper (with a title).  HMWK: make a summary of your findings (from today’s research) that you will turn in 10/7/14.

28. Day 4 – cell membrane research shareout  After each person shares what they learned about the cell membrane, fill in the following chart: PresenterI learned:I am unclear on: