-Types of Solutions -Facilitated Diffusion -Active Transport
1. What is diffusion? 2. What is osmosis? 3. Draw a picture of the same concentration of solutes inside a cell as outside a cell. 4. Draw a hyper person. Have data out and ready to hand in
Is also IMPERMEABLE to sucrose… Which is good, because I made a bunch of sucrose solutions of different concentrations, and I can’t remember which is which.
Objective:To determine which of the unknown solutions is High, Medium and Low concentration. Purpose: To observe the process of osmosis first hand and to use this process to understand how dialysis works.
3 Unknown colored solutions (High, Med and Low Concentration) Water (150 mL) 3 beakers* 3 pieces of dialysis tubing* 3 pipettes Scissors Scale Paper towel PROCEDURES: Watch demo, and write down procedures
Lab Title: Osmosis Lab (+ catchy title) Problem/Question: Does ______________ of _________ in the bag affect the final ________ of the bag in ___ after in soaks in water. Hypoth: if _________________ (color solution) has (highest/lowest solute concentration) relative to the Then _________________ As measured by __________
COLORINITIALFINALDifferenceHigh/Low /Med Red Blue Yellow Diagram: Draw all 3 set ups at the start of the experiment and 15 minutes later. -Include particulate drawings showing H20, and sucrose molecules. -Label the solute and the solvent. -Use arrows to show the flow of water
Iso – same Hypo – Low Hyper – High Tonic – Liquid/Solution
Hyper= more/too much Higher concentration solution outside the cell
Iso= same Same concentration outside the cell as inside
Hypo= less/too little Lower concentration solution outside the cell
Oxygen and Carbon dioxide enter and leave the cell through simple diffusion
Small unpolarized (not ions) molecules can pass through the cell membrane without a protein channel.
Lets revisit our salt water example According to simple diffusion, draw what will happen here.
The solute will spread out until it is evenly dispersed.
Draw what happens to a cell with lots of solute in it, dropped in a beaker of pure H 2 O? Remember that the cell membrane is permeable to water but not solutes.
Water follows solute into the cell… and the cell swells up.
If it swells up too much, the cell can burst if the membrane gets pulled apart.
FACILITATED DIFFUSION (another easy way – NO energy required!) Glucose, sodium ions and chloride ions molecules Glucose, sodium ions and chloride ions need help (facilitated) getting across membrane Carrier proteins help
Energy Energy (ATP) required for movement. AGAINST Solutes are moved AGAINST the concentration gradient. (from low to high conc.)
Look back at your notes for Passive and Active Transport. List two differences between passive transport and active transport. Name one thing they have in common!
Differences Active Uses ATP- This is the energy source for the cell. Active goes against the gradient Similar Both have carrier proteins Both move molecules that cannot go through the membrane on their own
Endocytosis- cell takes in substances Exocytosis- cell releases substances (out) ACTIVE TRANSPORT – requires ATP (energy)
A portion of the cell membrane surrounds the desired molecule outside the cell. The cell membrane rejoins pinching off a sac-like organelle called a vesicle. There are 2 types of endocytosis: phagocytosis and pinocytosis.
The reverse of endocytosis Wastes and cell products are packaged by the golgi body in sacs called vesicles. These vesicles fuse with the cell membrane and the materials are secreted out of the cell.
Cellular Transport Requires ______ Passive Transport: Diffusion: Osmosis: Hypertonic solution: Hypotonic solution: Isotonic solution: Facilitated diffusion: Glucose transport: Active Transport: Na+/K+ Pump: Osmosis Facilitated Diffusion Glucose transport Hypertonic Hypotonic Isotonic Oxygen & Carbon Dioxide Diffusion Transport PASSIVE Endocytosis Exocytosis ACTIVE Proteins and ATP Na+/K+ Pump Moves with gradient Moves against gradient ATP
Your Crazy Study Page For Mon’ Test Don’t forget: Draw a phospholipid bilayer with proteins and carbohydrates (& their functions) Solution: Solute-particle Solvent-liquid DiffusionOsmosis Passive vs. Active ENDO EXO
1. What is the difference between active and passive transport? 2. LIST 2 kinds of passive transport. 3. With a concentration gradient, molecules move from areas of ____ concentration to areas of ____ conc. 4. During osmosis, if there is more salt inside the cell than outside, which way would water move?