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Molecular Biology History or Human Genome Approximately what proportion of the DNA in the human genome codes for proteins or functional RNA? – A. 83% –

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Presentation on theme: "Molecular Biology History or Human Genome Approximately what proportion of the DNA in the human genome codes for proteins or functional RNA? – A. 83% –"— Presentation transcript:

1 Molecular Biology History or Human Genome Approximately what proportion of the DNA in the human genome codes for proteins or functional RNA? – A. 83% – B. 46% – C. 32% – D. 13% – E. 2%

2 Human Genome Which of the following techniques is considered the most significant development in the investigation of genetic disease since the completion of the human genome project? A.G-banding B.Fluorescent in situ hybridization C.DNA sequencing D.Array CGH E.Polymerase chain reactions

3 Human Genome Study of _______ marked the beginning of the revolution in biotechnology. A.thermal stable DNA polymerase B.RNA polymerase C.restriction endonucleases D.reverse transcriptase E.telomerase

4 Human Chromosomes In 1959, the chromosome abnormality of ______ was identified. A.cri du chat syndrome B.Down syndrome C.chronic myeloid leukemia D.breast cancer E.B and C are correct.

5 Human Chromosomes In 1959, the chromosome abnormality of ______ was identified. A.cri du chat syndrome B.Down syndrome C.chronic myeloid leukemia D.breast cancer E.B and C are correct.

6 Human Chromosomes In 1959, the chromosome abnormality of ______ was identified. A.cri du chat syndrome B.Down syndrome C.chronic myeloid leukemia D.breast cancer E.B and C are correct.

7 Human Chromosomes Which of the following statements about telomeric and subtelomeric regions is correct? A.The larger chromosomes have longer telomeric sequences. B.The telomeric regions are gene rich regions. C.The subtelomeric regions stain dark by using G-banding technique. D.The subtelomeric regions are gene rich.

8 Human Genome Study of _______ marked the beginning of the revolution in biotechnology. A.thermal stable DNA polymerase B.RNA polymerase C.restriction endonucleases D.reverse transcriptase E.telomerase

9 Human Genome Approximately what proportion of the DNA in the human genome codes for proteins or functional RNA? A.83% B.46% C.32% D.13% E.2%

10 Human Genome If proteins were If proteins were composed of only 12 different kinds of amino acids, what would be the smallest possible codon size in a genetic system with four different nucleotides? –A. 1 –B. 2 –C. 3 –D. 4 –E. 12

11 Human Genome The completion The completion of the Human Genome Project revealed that we have fewer genes than expected, not so many more than simpler organisms. The explanation(s) to this is(are): A. mRNA in humans are more likely to undergo alternative splicing. B. Post-translational modification adds diversity to the protein products. C. Polypeptide chains are combined in a variety ways. D. Regulation of gene expression in humans are more complex than those in other organisms. E. All of the above

12 InvestigatorsInvestigators in DNA and/or RNA Discovery Chemicals from heat-killed Smooth cells were purified. The chemicals were tested for the ability to transform live Rough cells. The transforming agent was found to be DNA –A. Griffith –B. Hershey and Chase –C. Avery, MacLeod, and McCarty –D. Chargaff –E. Meselson and Stahl

13 InvestigatorsInvestigators in DNA and/or RNA Discovery In any DNA sample, the amount of adenine equals the amount of thymine and the amount of guanine equals to the amount of cytosine. –A. Griffith –B. Hershey and Chase –C. Avery, MacLeod, and McCarty –D. Chargaff –E. Meselson and Stahl

14 InvestigatorsInvestigators in DNA and/or RNA Discovery The DNA of a phage was injected into the bacterial host, but the protein coat stayed outside. The viral DNA directed the host to replicate new phage viruses. –A. Griffith –B. Hershey and Chase –C. Avery, MacLeod, and McCarty –D. Chargaff –E. Meselson and Stahl

15 InvestigatorsInvestigators in DNA and/or RNA Discovery Using a short string of “UUUUUUUUUUUU” oligonucleotide, this scientist discovered that the genetic codon “UUU” specifies for phenylalanine. –A. James Watson –B. Francis Crick –C. Francis Colin –D. Craig Venter –E. Marshall Nirenberg

16 DNA Replication A KSU student A KSU student John analyzed a sample from Mars, using Meselson and Stahl’s method. He found that only a single DNA band appeared at the end of the CsCl centrifugation. Based on his data, we can say that the DNA on Mars is replicated in a____ fashion. A. conservative B. semiconservative C. dispersive D. It is difficult to come to the conclusion.

17 DNA Replication All of the following All of the following are functions of DNA polymerase in DNA replication, except A. covalently adding nucleotides to the new strands. B. proofreading each added nucleotide for correct base paring. C. replacing RNA primers with DNA. D. initiating a polynucleotide strand.

18 DNA Replication What is the role What is the role of DNA ligase in the elongation of the lagging strand during DNA replication? A. synthesize RNA nucleotides to make a primer B. catalyze the lengthening of telomeres C. join Okazaki fragments together D. stabilize the unwound parental DNA

19 Transcription/translation If proteins were If proteins were composed of only 12 different kinds of amino acids, what would be the smallest possible codon size in a genetic system with four different nucleotides? –A. 1 –B. 2 –C. 3 –D. 4 –E. 12


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